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The map label for this gene is napB [H]

Identifier: 209550514

GI number: 209550514

Start: 2994813

End: 2995316

Strand: Reverse

Name: napB [H]

Synonym: Rleg2_2937

Alternate gene names: 209550514

Gene position: 2995316-2994813 (Counterclockwise)

Preceding gene: 209550515

Following gene: 209550513

Centisome position: 66.01

GC content: 61.9

Gene sequence:

>504_bases
ATGCGCGGTCAAGATTCTCCCCGTCGTCGGTTGAGACGACCCGAATGGACAGCCATTGCAGCGGGTTTGCTGGTGTTCGT
CACGACGGCATCGATCGCTCAGGTCGTCACCCAGAATGTGCCCCAGCTTCAGGGGCCGCCTGAGGCCATGCAGTCCGCGC
CCGCCGCCGTCCTCCCGCGATGGGTGGTCGATGACCGCCGCCAGATGCGCGCCTATCCCGACCAGCCGCCTATTATCCCG
CATTCGATCGAGGGTTATGAGCTTTCGGTCAACGCGAACCGGTGCCTCTCCTGTCACAAGCGGGAATTCACTCAGGATTC
CGGCGCCCCGATGATCAGCGTCACCCACTACATGACGCGGGACGGCCAGATGATCGCCGATGTCTCGCCCCGGCGCTATT
TCTGCACGGCCTGCCACGTTCCCCAGGCTGACATCAAACCGTTGGTGCCAAGCATGTTCAAGGATATGAGCGAACTTGGT
TTCAAGCCTGCCGGAAGCGAGTGA

Upstream 100 bases:

>100_bases
CCGGCGTCATCTTCGTCCCGTGGTTCGACGCCAGCCAATTGATCAACAAGGTCACGCTCGACGCCACCGATCCCATCTCG
AAACAGACGGATTTCAAGAA

Downstream 100 bases:

>100_bases
TGACGATGATCGCTTGGGTGAAACACCTCATTCTCTGGGCATGGTCAGTCCTGACGACGCCGGCCGCGACGATCGGATTG
GGCGTGCTCACGCTCGGCGG

Product: nitrate reductase cytochrome c-type subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: Cytochrome c-552; Cytochrome c552 [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MRGQDSPRRRLRRPEWTAIAAGLLVFVTTASIAQVVTQNVPQLQGPPEAMQSAPAAVLPRWVVDDRRQMRAYPDQPPIIP
HSIEGYELSVNANRCLSCHKREFTQDSGAPMISVTHYMTRDGQMIADVSPRRYFCTACHVPQADIKPLVPSMFKDMSELG
FKPAGSE

Sequences:

>Translated_167_residues
MRGQDSPRRRLRRPEWTAIAAGLLVFVTTASIAQVVTQNVPQLQGPPEAMQSAPAAVLPRWVVDDRRQMRAYPDQPPIIP
HSIEGYELSVNANRCLSCHKREFTQDSGAPMISVTHYMTRDGQMIADVSPRRYFCTACHVPQADIKPLVPSMFKDMSELG
FKPAGSE
>Mature_167_residues
MRGQDSPRRRLRRPEWTAIAAGLLVFVTTASIAQVVTQNVPQLQGPPEAMQSAPAAVLPRWVVDDRRQMRAYPDQPPIIP
HSIEGYELSVNANRCLSCHKREFTQDSGAPMISVTHYMTRDGQMIADVSPRRYFCTACHVPQADIKPLVPSMFKDMSELG
FKPAGSE

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=139, Percent_Identity=43.1654676258993, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 18648; Mature: 18648

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGQDSPRRRLRRPEWTAIAAGLLVFVTTASIAQVVTQNVPQLQGPPEAMQSAPAAVLPR
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHH
WVVDDRRQMRAYPDQPPIIPHSIEGYELSVNANRCLSCHKREFTQDSGAPMISVTHYMTR
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEEEHHHHCC
DGQMIADVSPRRYFCTACHVPQADIKPLVPSMFKDMSELGFKPAGSE
CCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRGQDSPRRRLRRPEWTAIAAGLLVFVTTASIAQVVTQNVPQLQGPPEAMQSAPAAVLPR
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHH
WVVDDRRQMRAYPDQPPIIPHSIEGYELSVNANRCLSCHKREFTQDSGAPMISVTHYMTR
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCEEEEHHHHCC
DGQMIADVSPRRYFCTACHVPQADIKPLVPSMFKDMSELGFKPAGSE
CCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7639719; 8119278; 8194605 [H]