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The map label for this gene is napA [H]

Identifier: 209550515

GI number: 209550515

Start: 2995283

End: 2997790

Strand: Reverse

Name: napA [H]

Synonym: Rleg2_2938

Alternate gene names: 209550515

Gene position: 2997790-2995283 (Counterclockwise)

Preceding gene: 209550516

Following gene: 209550514

Centisome position: 66.06

GC content: 61.32

Gene sequence:

>2508_bases
ATGACCAGTGAAGTGACCCGTCGTGACATCCTGAAGGCGCACGCGGCAGCGATAGCCGCGGCGACCGCGGGCATAACGCT
TCCGGCCGCCGCGCAGCAGGTGCCCGGCGGCATCGATGCGCTCGAGATAAAGTGGTCGAAAGCGCCCTGCAGGTTTTGCG
GGACCGGCTGTGGCGTCATGGTCGGGGTCAAGCAGGGCCAGGTCGTCGCCACGCACGGCGACATGCAGGCCGAAGTGAAC
CGGGGCCTGAACTGTATCAAGGGCTACTTCCTTTCGAAGATCATGTACGGCCAGGACCGGTTGAAGACGCCGCTGCTGCG
CAAGCAGAACGGCGTCTACGCCAAGGAAGGCGAGTTCGAGCCGGTGAGCTGGGATGAGGCCTTTGACGTGATGGCCGCGC
AGTGCAAACGCGTTCTGAAGGAGAAGGGGCCGACCGCCATCGGCATGTTCGGCTCCGGCCAGTGGACGATCTTCGAAGGG
TACGCCGCCACGAAACTGATGCGCGCCGGCTTCCGGTCGAACAATCTCGATCCAAACGCCCGCCACTGCATGGCCTCTGC
CGCCGTCGCCTTCATGCGAACCTTCGGCATGGACGAGCCGATGGGATGCTACGACGATTTCGAGAATGCCGACGCCTTCG
TGCTTTGGGGCTCCAACATGGCGGAGATGCATCCGATCCTGTGGACGCGGGTCGCCGATCGTCGCCTCGGCCAACCACAT
GTCCGGGTCGCGGTGTTGTCCACCTTCACCCATCGCAGCATGGATCTGGCCGACATACCCATCGTCTTCAAGCCCGGCAC
TGACTTGGTGATCCTGAACTATATCGCCAACCACATCATTCAAACCGGGCGGGTCAACGAGGCATTCGTTGAGAAGCATG
CCAAATTTGCCCGCGCCGCGACCGATATCGGCTACGGCCTGCGCCCCGATGATCCGATCGAGGTCAAGGCCGCAAATTCG
GCCGATCCGGGCAAGACCGAGCCGATGGATTTCGAGGCGTTCAAGGACTTCGTCTCGGATTACACGCTGAAGAAGACGGC
AGCGATGACCGGCGTCGATCCGGAGTTTCTCGAGCAGCTGGCCGAGCTTTATGCCGATCCCGCCCGCAAAGTCATGTCGC
TCTGGACGATGGGCTTCAATCAGCACGTCAGGGGCGTCTGGGCCAACCAGATGGTCTACAACCTCCATCTTCTGACGGGC
AAGATCGCCGAGCCGGGCAACAGTCCATTTTCCCTGACCGGTCAGCCTTCCGCTTGCGGTACGGCGCGCGAGGTCGGAAC
CTTCGCCCATCGCCTGCCTGCCGACATGGTGGTGACCAACCCCGAGCATCGCAAACATGCCGAGGAGATCTGGCACGTCC
CTCACGGCCTGATCCCGGAAAAACCCGGCTACCATGCGGTGCAGCAGGACCGGATGCTGAAGGATGGCAAGCTGAATTTC
TACTGGGTGCAGGTCAACAACAACGTCCAGGCGGCGCCCAATACCAGCAACGAGACCTATAACGGCTATCGCAATCCTGA
AAACTTCATCGTCGTCTCCGACGCCTATCCGACGATCACCGCGATGAGTGCCGACCTGATTTTGCCCGCCGCCATGTGGG
TGGAAAAGGAAGGCGCCTACGGCAACGCCGAACGCCGGACGCATGTGTGGCACCAGCTTGTGCAGGCGTCGGGCGAGGCG
CGCTCCGACCTCTGGCAGATGATGGAGTTCTCGAAGCGCTTCACCACCGACGAGGTCTGGCCGGCGGAGCTGCTGGACGC
CAATCCGACCTATCGCGGCAAGACGCTTTTCGACGTGCTGTTTCGCAACGGCGAGGTTGACCGGTACCCTCTCGAAGACA
TCAGTGCCGACTACGACAATAACGAGGCAAAGGCCTTCGGCTTCTATGTCCAGAAGGGGCTTTTCGAAGAGTATGCCGCA
TTCGGACGCGGACATGGCCACGATCTGGCGCCTTACGACGTCTATCACGAGGTTCGAGGCCTGCGCTGGCCCGTCGTCGA
CGGGCAGGAAACCAAATGGCGATACCGCGAAGGATACGACCCTTATGTCAAATCCGGCGAGGGTTTGAATTTCTACGGCC
AGGCGGATGGCAAGGCCGTCATCCTGGCAGCACCCTACGAGCCTCCGGCAGAATCGCCCGACGACGAGTTCGATGTCTGG
CTGGTGACGGGACGGGTGCTTGAACATTGGCATTCCGGTTCGATGACAATGCGGGTTCCCGAACTCTACAAGGCCTTCCC
CGGGGCGCGCTGTTTCATGAATGCCGACGACGCGCGAAAACGTGGCATCAACCAGGGGGCCGAAGTCCGCATCGTCTCGC
GGCGCGGTGAAATCCGCACCCGGATCGACACTCGCGGCAGAAACCGGATGCCATCCGGCGTCATCTTCGTCCCGTGGTTC
GACGCCAGCCAATTGATCAACAAGGTCACGCTCGACGCCACCGATCCCATCTCGAAACAGACGGATTTCAAGAAATGCGC
GGTCAAGATTCTCCCCGTCGTCGGTTGA

Upstream 100 bases:

>100_bases
CACAGGCATGATGGGCGAATGCCTGTCGCGCATATCGCTGTTCGATGGGGTCATCTCCGCGAATATGGTTTTCGAGCATG
TCGAAACGGAGGGGGCGTAG

Downstream 100 bases:

>100_bases
GACGACCCGAATGGACAGCCATTGCAGCGGGTTTGCTGGTGTTCGTCACGACGGCATCGATCGCTCAGGTCGTCACCCAG
AATGTGCCCCAGCTTCAGGG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 835; Mature: 834

Protein sequence:

>835_residues
MTSEVTRRDILKAHAAAIAAATAGITLPAAAQQVPGGIDALEIKWSKAPCRFCGTGCGVMVGVKQGQVVATHGDMQAEVN
RGLNCIKGYFLSKIMYGQDRLKTPLLRKQNGVYAKEGEFEPVSWDEAFDVMAAQCKRVLKEKGPTAIGMFGSGQWTIFEG
YAATKLMRAGFRSNNLDPNARHCMASAAVAFMRTFGMDEPMGCYDDFENADAFVLWGSNMAEMHPILWTRVADRRLGQPH
VRVAVLSTFTHRSMDLADIPIVFKPGTDLVILNYIANHIIQTGRVNEAFVEKHAKFARAATDIGYGLRPDDPIEVKAANS
ADPGKTEPMDFEAFKDFVSDYTLKKTAAMTGVDPEFLEQLAELYADPARKVMSLWTMGFNQHVRGVWANQMVYNLHLLTG
KIAEPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVTNPEHRKHAEEIWHVPHGLIPEKPGYHAVQQDRMLKDGKLNF
YWVQVNNNVQAAPNTSNETYNGYRNPENFIVVSDAYPTITAMSADLILPAAMWVEKEGAYGNAERRTHVWHQLVQASGEA
RSDLWQMMEFSKRFTTDEVWPAELLDANPTYRGKTLFDVLFRNGEVDRYPLEDISADYDNNEAKAFGFYVQKGLFEEYAA
FGRGHGHDLAPYDVYHEVRGLRWPVVDGQETKWRYREGYDPYVKSGEGLNFYGQADGKAVILAAPYEPPAESPDDEFDVW
LVTGRVLEHWHSGSMTMRVPELYKAFPGARCFMNADDARKRGINQGAEVRIVSRRGEIRTRIDTRGRNRMPSGVIFVPWF
DASQLINKVTLDATDPISKQTDFKKCAVKILPVVG

Sequences:

>Translated_835_residues
MTSEVTRRDILKAHAAAIAAATAGITLPAAAQQVPGGIDALEIKWSKAPCRFCGTGCGVMVGVKQGQVVATHGDMQAEVN
RGLNCIKGYFLSKIMYGQDRLKTPLLRKQNGVYAKEGEFEPVSWDEAFDVMAAQCKRVLKEKGPTAIGMFGSGQWTIFEG
YAATKLMRAGFRSNNLDPNARHCMASAAVAFMRTFGMDEPMGCYDDFENADAFVLWGSNMAEMHPILWTRVADRRLGQPH
VRVAVLSTFTHRSMDLADIPIVFKPGTDLVILNYIANHIIQTGRVNEAFVEKHAKFARAATDIGYGLRPDDPIEVKAANS
ADPGKTEPMDFEAFKDFVSDYTLKKTAAMTGVDPEFLEQLAELYADPARKVMSLWTMGFNQHVRGVWANQMVYNLHLLTG
KIAEPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVTNPEHRKHAEEIWHVPHGLIPEKPGYHAVQQDRMLKDGKLNF
YWVQVNNNVQAAPNTSNETYNGYRNPENFIVVSDAYPTITAMSADLILPAAMWVEKEGAYGNAERRTHVWHQLVQASGEA
RSDLWQMMEFSKRFTTDEVWPAELLDANPTYRGKTLFDVLFRNGEVDRYPLEDISADYDNNEAKAFGFYVQKGLFEEYAA
FGRGHGHDLAPYDVYHEVRGLRWPVVDGQETKWRYREGYDPYVKSGEGLNFYGQADGKAVILAAPYEPPAESPDDEFDVW
LVTGRVLEHWHSGSMTMRVPELYKAFPGARCFMNADDARKRGINQGAEVRIVSRRGEIRTRIDTRGRNRMPSGVIFVPWF
DASQLINKVTLDATDPISKQTDFKKCAVKILPVVG
>Mature_834_residues
TSEVTRRDILKAHAAAIAAATAGITLPAAAQQVPGGIDALEIKWSKAPCRFCGTGCGVMVGVKQGQVVATHGDMQAEVNR
GLNCIKGYFLSKIMYGQDRLKTPLLRKQNGVYAKEGEFEPVSWDEAFDVMAAQCKRVLKEKGPTAIGMFGSGQWTIFEGY
AATKLMRAGFRSNNLDPNARHCMASAAVAFMRTFGMDEPMGCYDDFENADAFVLWGSNMAEMHPILWTRVADRRLGQPHV
RVAVLSTFTHRSMDLADIPIVFKPGTDLVILNYIANHIIQTGRVNEAFVEKHAKFARAATDIGYGLRPDDPIEVKAANSA
DPGKTEPMDFEAFKDFVSDYTLKKTAAMTGVDPEFLEQLAELYADPARKVMSLWTMGFNQHVRGVWANQMVYNLHLLTGK
IAEPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVTNPEHRKHAEEIWHVPHGLIPEKPGYHAVQQDRMLKDGKLNFY
WVQVNNNVQAAPNTSNETYNGYRNPENFIVVSDAYPTITAMSADLILPAAMWVEKEGAYGNAERRTHVWHQLVQASGEAR
SDLWQMMEFSKRFTTDEVWPAELLDANPTYRGKTLFDVLFRNGEVDRYPLEDISADYDNNEAKAFGFYVQKGLFEEYAAF
GRGHGHDLAPYDVYHEVRGLRWPVVDGQETKWRYREGYDPYVKSGEGLNFYGQADGKAVILAAPYEPPAESPDDEFDVWL
VTGRVLEHWHSGSMTMRVPELYKAFPGARCFMNADDARKRGINQGAEVRIVSRRGEIRTRIDTRGRNRMPSGVIFVPWFD
ASQLINKVTLDATDPISKQTDFKKCAVKILPVVG

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=830, Percent_Identity=66.2650602409639, Blast_Score=1174, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=800, Percent_Identity=24.875, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI3868720, Length=609, Percent_Identity=22.495894909688, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI3868719, Length=476, Percent_Identity=22.0588235294118, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081797, Length=572, Percent_Identity=24.1258741258741, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI171474008, Length=602, Percent_Identity=23.0897009966777, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787870, Length=602, Percent_Identity=23.0897009966777, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93287; Mature: 93156

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEVTRRDILKAHAAAIAAATAGITLPAAAQQVPGGIDALEIKWSKAPCRFCGTGCGVM
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHCCCCCEE
VGVKQGQVVATHGDMQAEVNRGLNCIKGYFLSKIMYGQDRLKTPLLRKQNGVYAKEGEFE
EEECCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHCCHHHCCCHHHCCCCCEECCCCCC
PVSWDEAFDVMAAQCKRVLKEKGPTAIGMFGSGQWTIFEGYAATKLMRAGFRSNNLDPNA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECHHHHHHHHHCCCCCCCCCHH
RHCMASAAVAFMRTFGMDEPMGCYDDFENADAFVLWGSNMAEMHPILWTRVADRRLGQPH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCC
VRVAVLSTFTHRSMDLADIPIVFKPGTDLVILNYIANHIIQTGRVNEAFVEKHAKFARAA
EEEEEEEHHHHCCCCCCCCCEEECCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TDIGYGLRPDDPIEVKAANSADPGKTEPMDFEAFKDFVSDYTLKKTAAMTGVDPEFLEQL
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
AELYADPARKVMSLWTMGFNQHVRGVWANQMVYNLHLLTGKIAEPGNSPFSLTGQPSACG
HHHHHCHHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCEEECCCCCCCC
TAREVGTFAHRLPADMVVTNPEHRKHAEEIWHVPHGLIPEKPGYHAVQQDRMLKDGKLNF
CHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEE
YWVQVNNNVQAAPNTSNETYNGYRNPENFIVVSDAYPTITAMSADLILPAAMWVEKEGAY
EEEEECCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEHCCHHHHHHHHHHEECCCCC
GNAERRTHVWHQLVQASGEARSDLWQMMEFSKRFTTDEVWPAELLDANPTYRGKTLFDVL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHH
FRNGEVDRYPLEDISADYDNNEAKAFGFYVQKGLFEEYAAFGRGHGHDLAPYDVYHEVRG
HHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
LRWPVVDGQETKWRYREGYDPYVKSGEGLNFYGQADGKAVILAAPYEPPAESPDDEFDVW
CCCCCCCCCCCCCHHHCCCCHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEEE
LVTGRVLEHWHSGSMTMRVPELYKAFPGARCFMNADDARKRGINQGAEVRIVSRRGEIRT
EEEHHHHHHHCCCCEEEECHHHHHHCCCCEEEECCCHHHHHCCCCCCEEEEEECCCCEEE
RIDTRGRNRMPSGVIFVPWFDASQLINKVTLDATDPISKQTDFKKCAVKILPVVG
ECCCCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCCCCCCHHHHHHHHEECCCC
>Mature Secondary Structure 
TSEVTRRDILKAHAAAIAAATAGITLPAAAQQVPGGIDALEIKWSKAPCRFCGTGCGVM
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHCCCCCEE
VGVKQGQVVATHGDMQAEVNRGLNCIKGYFLSKIMYGQDRLKTPLLRKQNGVYAKEGEFE
EEECCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHCCHHHCCCHHHCCCCCEECCCCCC
PVSWDEAFDVMAAQCKRVLKEKGPTAIGMFGSGQWTIFEGYAATKLMRAGFRSNNLDPNA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECHHHHHHHHHCCCCCCCCCHH
RHCMASAAVAFMRTFGMDEPMGCYDDFENADAFVLWGSNMAEMHPILWTRVADRRLGQPH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCC
VRVAVLSTFTHRSMDLADIPIVFKPGTDLVILNYIANHIIQTGRVNEAFVEKHAKFARAA
EEEEEEEHHHHCCCCCCCCCEEECCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TDIGYGLRPDDPIEVKAANSADPGKTEPMDFEAFKDFVSDYTLKKTAAMTGVDPEFLEQL
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
AELYADPARKVMSLWTMGFNQHVRGVWANQMVYNLHLLTGKIAEPGNSPFSLTGQPSACG
HHHHHCHHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCEEECCCCCCCC
TAREVGTFAHRLPADMVVTNPEHRKHAEEIWHVPHGLIPEKPGYHAVQQDRMLKDGKLNF
CHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEE
YWVQVNNNVQAAPNTSNETYNGYRNPENFIVVSDAYPTITAMSADLILPAAMWVEKEGAY
EEEEECCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEHCCHHHHHHHHHHEECCCCC
GNAERRTHVWHQLVQASGEARSDLWQMMEFSKRFTTDEVWPAELLDANPTYRGKTLFDVL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHH
FRNGEVDRYPLEDISADYDNNEAKAFGFYVQKGLFEEYAAFGRGHGHDLAPYDVYHEVRG
HHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
LRWPVVDGQETKWRYREGYDPYVKSGEGLNFYGQADGKAVILAAPYEPPAESPDDEFDVW
CCCCCCCCCCCCCHHHCCCCHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEEE
LVTGRVLEHWHSGSMTMRVPELYKAFPGARCFMNADDARKRGINQGAEVRIVSRRGEIRT
EEEHHHHHHHCCCCEEEECHHHHHHCCCCEEEECCCHHHHHCCCCCCEEEEEECCCCEEE
RIDTRGRNRMPSGVIFVPWFDASQLINKVTLDATDPISKQTDFKKCAVKILPVVG
ECCCCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCCCCCCHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]