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The map label for this gene is dctA [H]

Identifier: 209550297

GI number: 209550297

Start: 2762926

End: 2764257

Strand: Reverse

Name: dctA [H]

Synonym: Rleg2_2717

Alternate gene names: 209550297

Gene position: 2764257-2762926 (Counterclockwise)

Preceding gene: 209550300

Following gene: 209550294

Centisome position: 60.91

GC content: 62.24

Gene sequence:

>1332_bases
ATGATCGCAGCACCATTCGATGCAGTCGCAGACAGCACGGGCAAGAAGCCCTTTTATTCCCATCTCTACGTTCAGGTTCT
CGCCGCCATCGCCGCGGGTATCCTTCTCGGCCATTTCTATCCCGAGCTCGGCGCGCAGCTGAAGCCGCTCGGCGATGCCT
TCATCAAGCTCGTCAAGATGATCATCGCTCCGGTGATCTTCCTGACGGTGGCGACCGGCATTGCCGGCATGAGCGACCTG
AAGAAGGTCGGCCGCGTCGCCGGCAAGGCGATGCTGTACTTCCTGACCTTCTCGACCTTGGCGCTAGTCATCGGCATGGT
CGTTGCCAACGTCGTCCAGCCCGGCGCCGGCATGAATATCGATCCGGCGTCGCTCGATCCGACCGCCGTCGCCACCTACG
CTTCGAAGGCGCATGAGCAGAGCATCGTCGGCTTCCTCACCAATATCATTCCGACGACGATCGTCGGTGCCTTTGCCGAT
GGCGACATTCTGCAGGTGCTGTTCTTCTCGGTGCTCTTCGGCATCGCGCTTGCCATGGTCGGCGAAAAGGGTGAGCAGGT
CGTCAATTTCCTGAACGCCCTGACGGCTCCGGTCTTCAAGCTCGTCGCCATCCTGATGAAGGCTGCCCCGATCGGCGCTT
TCGGCGCCATGGCCTTCACCATCGGCAAATACGGCATCGGATCGATCGCCAATCTCGCCATGCTGATCGGCACTTTCTAC
CTCACCTCGCTGCTCTTCGTTCTCGTCGTTCTCGGCGCCGTCGCCCGCTATAACGGCTTCTCGATCCTGGCGCTGCTGCG
CTACATCAAGGAAGAGCTGCTGCTGGTTCTCGGCACCTCGTCTTCCGAAGCCGCACTTCCGGGCCTGATGAACAAGATGG
AAAAGGCCGGCTGCAAGCGTTCGGTCGTCGGCCTCGTCATCCCGACAGGCTATTCCTTCAATCTCGACGGCACCAACATC
TACATGACGCTGGCAGCGCTGTTCATCGCCCAGGCAACCGGCATCAACCTCTCCTGGGGTGACCAGATCCTGCTGCTCCT
GGTGGCGATGCTGAGCTCGAAGGGTGCCGCCGGCATCACCGGCGCCGGCTTCATCACCCTTGCCGCCACGCTCTCCGTCG
TGCCGTCCGTGCCGGTCGCCGGCATGGCGCTGATCCTCGGCATCGACCGCTTCATGTCGGAATGCCGGGCGCTGACCAAC
CTCGTCGGCAATGCCGTGGCGACGATCGTCGTGGCACGCTGGGAAAACGAACTGGATACCGCGCAGCTCGCCAGAGCCCT
GGGCGGCCAGGCAGAAGAGAGCGCTCCGGCCGGACTGCAGCCTGCGGAATAA

Upstream 100 bases:

>100_bases
GTGATTGCGAAGGAGATGGCAACAACGGCTGAGCTGTTGGATTGAAGCGAACGGCTCGGGAGGCCGGAGTTCGTTCCGGA
CGAGCCTTAAGGAGGACATC

Downstream 100 bases:

>100_bases
TCAGCGATTGCTGGCAATCTTTGCAGCGGCCCGTTATCTGACGGGCCGTTCGCTTGTTTATAAACTCGATCTCTACTCGA
CGTCATCCTGGGCGTTGTGC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIAAPFDAVADSTGKKPFYSHLYVQVLAAIAAGILLGHFYPELGAQLKPLGDAFIKLVKMIIAPVIFLTVATGIAGMSDL
KKVGRVAGKAMLYFLTFSTLALVIGMVVANVVQPGAGMNIDPASLDPTAVATYASKAHEQSIVGFLTNIIPTTIVGAFAD
GDILQVLFFSVLFGIALAMVGEKGEQVVNFLNALTAPVFKLVAILMKAAPIGAFGAMAFTIGKYGIGSIANLAMLIGTFY
LTSLLFVLVVLGAVARYNGFSILALLRYIKEELLLVLGTSSSEAALPGLMNKMEKAGCKRSVVGLVIPTGYSFNLDGTNI
YMTLAALFIAQATGINLSWGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGIDRFMSECRALTN
LVGNAVATIVVARWENELDTAQLARALGGQAEESAPAGLQPAE

Sequences:

>Translated_443_residues
MIAAPFDAVADSTGKKPFYSHLYVQVLAAIAAGILLGHFYPELGAQLKPLGDAFIKLVKMIIAPVIFLTVATGIAGMSDL
KKVGRVAGKAMLYFLTFSTLALVIGMVVANVVQPGAGMNIDPASLDPTAVATYASKAHEQSIVGFLTNIIPTTIVGAFAD
GDILQVLFFSVLFGIALAMVGEKGEQVVNFLNALTAPVFKLVAILMKAAPIGAFGAMAFTIGKYGIGSIANLAMLIGTFY
LTSLLFVLVVLGAVARYNGFSILALLRYIKEELLLVLGTSSSEAALPGLMNKMEKAGCKRSVVGLVIPTGYSFNLDGTNI
YMTLAALFIAQATGINLSWGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGIDRFMSECRALTN
LVGNAVATIVVARWENELDTAQLARALGGQAEESAPAGLQPAE
>Mature_443_residues
MIAAPFDAVADSTGKKPFYSHLYVQVLAAIAAGILLGHFYPELGAQLKPLGDAFIKLVKMIIAPVIFLTVATGIAGMSDL
KKVGRVAGKAMLYFLTFSTLALVIGMVVANVVQPGAGMNIDPASLDPTAVATYASKAHEQSIVGFLTNIIPTTIVGAFAD
GDILQVLFFSVLFGIALAMVGEKGEQVVNFLNALTAPVFKLVAILMKAAPIGAFGAMAFTIGKYGIGSIANLAMLIGTFY
LTSLLFVLVVLGAVARYNGFSILALLRYIKEELLLVLGTSSSEAALPGLMNKMEKAGCKRSVVGLVIPTGYSFNLDGTNI
YMTLAALFIAQATGINLSWGDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGIDRFMSECRALTN
LVGNAVATIVVARWENELDTAQLARALGGQAEESAPAGLQPAE

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=457, Percent_Identity=28.0087527352298, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI169790839, Length=448, Percent_Identity=26.3392857142857, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI194239697, Length=499, Percent_Identity=25.6513026052104, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI66773030, Length=396, Percent_Identity=26.2626262626263, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI21314632, Length=404, Percent_Identity=26.7326732673267, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI4827012, Length=394, Percent_Identity=27.1573604060914, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI5032093, Length=301, Percent_Identity=31.5614617940199, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI223468566, Length=287, Percent_Identity=31.3588850174216, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI223468564, Length=257, Percent_Identity=32.295719844358, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI262359914, Length=370, Percent_Identity=25.6756756756757, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI301601644, Length=166, Percent_Identity=28.3132530120482, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789947, Length=419, Percent_Identity=62.5298329355609, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1790514, Length=428, Percent_Identity=39.4859813084112, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788024, Length=402, Percent_Identity=24.8756218905473, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789473, Length=323, Percent_Identity=22.9102167182663, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=391, Percent_Identity=26.5984654731458, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71983099, Length=460, Percent_Identity=25.4347826086956, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=460, Percent_Identity=25.4347826086956, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71996953, Length=424, Percent_Identity=27.122641509434, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI193206654, Length=461, Percent_Identity=25.3796095444685, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI193206505, Length=440, Percent_Identity=25.6818181818182, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=431, Percent_Identity=22.969837587007, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24583025, Length=435, Percent_Identity=24.5977011494253, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17137668, Length=435, Percent_Identity=24.5977011494253, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24583023, Length=435, Percent_Identity=24.5977011494253, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281360481, Length=476, Percent_Identity=24.7899159663866, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281360483, Length=476, Percent_Identity=24.7899159663866, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137666, Length=476, Percent_Identity=24.7899159663866, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46025; Mature: 46025

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAAPFDAVADSTGKKPFYSHLYVQVLAAIAAGILLGHFYPELGAQLKPLGDAFIKLVKM
CCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IIAPVIFLTVATGIAGMSDLKKVGRVAGKAMLYFLTFSTLALVIGMVVANVVQPGAGMNI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DPASLDPTAVATYASKAHEQSIVGFLTNIIPTTIVGAFADGDILQVLFFSVLFGIALAMV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GEKGEQVVNFLNALTAPVFKLVAILMKAAPIGAFGAMAFTIGKYGIGSIANLAMLIGTFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LTSLLFVLVVLGAVARYNGFSILALLRYIKEELLLVLGTSSSEAALPGLMNKMEKAGCKR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCHH
SVVGLVIPTGYSFNLDGTNIYMTLAALFIAQATGINLSWGDQILLLLVAMLSSKGAAGIT
CEEEEEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
GAGFITLAATLSVVPSVPVAGMALILGIDRFMSECRALTNLVGNAVATIVVARWENELDT
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AQLARALGGQAEESAPAGLQPAE
HHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA