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The map label for this gene is kbl [C]

Identifier: 209550281

GI number: 209550281

Start: 2739592

End: 2740779

Strand: Reverse

Name: kbl [C]

Synonym: Rleg2_2701

Alternate gene names: 209550281

Gene position: 2740779-2739592 (Counterclockwise)

Preceding gene: 209550282

Following gene: 209550280

Centisome position: 60.4

GC content: 62.21

Gene sequence:

>1188_bases
ATGACCTCGCCATTTCTCTCCCATCTCAGGGCCGAAATTTCGGCGCTGAAGGATGCCGGCCTCTATAAATCCGAGCGGGT
GATCAGCTCCAAGCAGGCAGGCGAGATTGCGATTTCGACCGGCGAGCGGGTGCTGAATTTCTGCGCCAACAACTATCTCG
GCCTTGCCGACAATGAGGAGCTGGCCGTGGCCGGCAAGCAGGCGCTCGACCGCTACGGCTACGGCATGGCCTCGGTGCGC
TTCATCTGCGGCACGCAGGAGGAGCATAAGCAGCTCGAAGCGCGCATTTCCGCCTTTCTCGGCATGGAAGACACGATCCT
TTACTCCTCCTGCTTCGATGCCAATGGCGGTCTGTTCGAAACGCTGCTCTCCGAGGAAGACGCGATCATCTCCGATGCGC
TGAACCACGCCTCGATCATCGACGGCGTCAGGCTGTCGAAGGCCAAGCGCTTCCGCTACGCCAACAACGATATGGCAGCC
CTCGAAGAGGAGCTGAAGAAGGCCGAAGGCAGCCGCTTCAAGCTGATCGCCACCGATGGCGTCTTCTCGATGGACGGCAT
CATCGCTAACTTGGGTGGCGTCTGCGATCTCGCTGAGAAATATGGCGCCATGGTCATGGTCGATGACAGCCATGCGGTCG
GTTTCGTCGGCAAGAACGGTCGTGGCTCGCCCGAACATTGCGGCGTCGAAGGCCGGATCGACATCATCACCGGCACGCTC
GGCAAGGCGCTCGGCGGCGCCTCCGGCGGTTATACCGCGGCGAAGGCCGAGGTCGTCGAATGGCTGCGGCAGCGCTCGCG
GCCCTATCTGTTCTCGAACACGCTGGCACCGGTCATCGCCGCCGCCTCGCTGAAAGTGTTCGACCTCATCGAAAACGGCG
ATGCCTTGCGCCAGCGCCTGTCCGACAATGCCGATCTCTTCCGCACCGAGATGACCAAGCTCGGATTCACTCTCGCCGGC
GAAGGCCATCCGATCATCCCGGTCATGCTCGGCGACGCCAAGCTCGCGCAGGACATGGCGGGGCTGATGCTGAAGAAGGG
CATTTACGTGATCGGCTTCTCCTTCCCGGTCGTGCCGAAGGGCCAGGCCCGCATCCGCACGCAAATGTCGGCTGCGCATT
CCAGGGCCGATGTGAAGCGGGCGATTGCGGCCTTTGCGGAAGCGGGGCGGGAACTGGGTGTGATTTGA

Upstream 100 bases:

>100_bases
CGGCCGATGTTCCTGCTGATCTCGGCCCGGCGGTGAAGAAGACAAGCCGGCGGCGATATGCTAACCCGAGTGCTATAAAA
AACATCAGGAAGGGCCGCCC

Downstream 100 bases:

>100_bases
GAATGAGGCTGCCCCTCACCCTAACCCTCTCCCCGTAAACGGGGCACGTGCCCTGCGAAATGATGGTGGGGACGGAGAGG
TCGCCGCTTGTCTCCTTCGC

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTSPFLSHLRAEISALKDAGLYKSERVISSKQAGEIAISTGERVLNFCANNYLGLADNEELAVAGKQALDRYGYGMASVR
FICGTQEEHKQLEARISAFLGMEDTILYSSCFDANGGLFETLLSEEDAIISDALNHASIIDGVRLSKAKRFRYANNDMAA
LEEELKKAEGSRFKLIATDGVFSMDGIIANLGGVCDLAEKYGAMVMVDDSHAVGFVGKNGRGSPEHCGVEGRIDIITGTL
GKALGGASGGYTAAKAEVVEWLRQRSRPYLFSNTLAPVIAAASLKVFDLIENGDALRQRLSDNADLFRTEMTKLGFTLAG
EGHPIIPVMLGDAKLAQDMAGLMLKKGIYVIGFSFPVVPKGQARIRTQMSAAHSRADVKRAIAAFAEAGRELGVI

Sequences:

>Translated_395_residues
MTSPFLSHLRAEISALKDAGLYKSERVISSKQAGEIAISTGERVLNFCANNYLGLADNEELAVAGKQALDRYGYGMASVR
FICGTQEEHKQLEARISAFLGMEDTILYSSCFDANGGLFETLLSEEDAIISDALNHASIIDGVRLSKAKRFRYANNDMAA
LEEELKKAEGSRFKLIATDGVFSMDGIIANLGGVCDLAEKYGAMVMVDDSHAVGFVGKNGRGSPEHCGVEGRIDIITGTL
GKALGGASGGYTAAKAEVVEWLRQRSRPYLFSNTLAPVIAAASLKVFDLIENGDALRQRLSDNADLFRTEMTKLGFTLAG
EGHPIIPVMLGDAKLAQDMAGLMLKKGIYVIGFSFPVVPKGQARIRTQMSAAHSRADVKRAIAAFAEAGRELGVI
>Mature_394_residues
TSPFLSHLRAEISALKDAGLYKSERVISSKQAGEIAISTGERVLNFCANNYLGLADNEELAVAGKQALDRYGYGMASVRF
ICGTQEEHKQLEARISAFLGMEDTILYSSCFDANGGLFETLLSEEDAIISDALNHASIIDGVRLSKAKRFRYANNDMAAL
EEELKKAEGSRFKLIATDGVFSMDGIIANLGGVCDLAEKYGAMVMVDDSHAVGFVGKNGRGSPEHCGVEGRIDIITGTLG
KALGGASGGYTAAKAEVVEWLRQRSRPYLFSNTLAPVIAAASLKVFDLIENGDALRQRLSDNADLFRTEMTKLGFTLAGE
GHPIIPVMLGDAKLAQDMAGLMLKKGIYVIGFSFPVVPKGQARIRTQMSAAHSRADVKRAIAAFAEAGRELGVI

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=390, Percent_Identity=54.8717948717949, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI284448556, Length=414, Percent_Identity=51.4492753623188, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI83977442, Length=333, Percent_Identity=36.3363363363363, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI83977440, Length=333, Percent_Identity=36.3363363363363, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI83977444, Length=333, Percent_Identity=36.3363363363363, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI4502025, Length=343, Percent_Identity=32.9446064139942, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI40316939, Length=343, Percent_Identity=32.9446064139942, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI119220554, Length=364, Percent_Identity=32.967032967033, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI4758668, Length=364, Percent_Identity=32.6923076923077, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI5454084, Length=280, Percent_Identity=28.9285714285714, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1790046, Length=397, Percent_Identity=67.5062972292191, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1786993, Length=351, Percent_Identity=31.3390313390313, Blast_Score=181, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI71994529, Length=397, Percent_Identity=56.9269521410579, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI32566772, Length=407, Percent_Identity=29.97542997543, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17560914, Length=354, Percent_Identity=31.638418079096, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17560912, Length=354, Percent_Identity=31.638418079096, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71982617, Length=286, Percent_Identity=26.5734265734266, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71982625, Length=286, Percent_Identity=26.5734265734266, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320438, Length=392, Percent_Identity=31.6326530612245, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320267, Length=368, Percent_Identity=29.6195652173913, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323954, Length=275, Percent_Identity=29.8181818181818, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24662918, Length=388, Percent_Identity=59.020618556701, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17137420, Length=348, Percent_Identity=31.8965517241379, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17136286, Length=381, Percent_Identity=31.496062992126, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24653280, Length=348, Percent_Identity=25.8620689655172, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653276, Length=348, Percent_Identity=25.8620689655172, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653278, Length=348, Percent_Identity=25.8620689655172, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 42371; Mature: 42240

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPFLSHLRAEISALKDAGLYKSERVISSKQAGEIAISTGERVLNFCANNYLGLADNEE
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCC
LAVAGKQALDRYGYGMASVRFICGTQEEHKQLEARISAFLGMEDTILYSSCFDANGGLFE
HHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCHHEEEHHCCCCCCCHHH
TLLSEEDAIISDALNHASIIDGVRLSKAKRFRYANNDMAALEEELKKAEGSRFKLIATDG
HHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECC
VFSMDGIIANLGGVCDLAEKYGAMVMVDDSHAVGFVGKNGRGSPEHCGVEGRIDIITGTL
CEEHHHHHHHHCHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECHH
GKALGGASGGYTAAKAEVVEWLRQRSRPYLFSNTLAPVIAAASLKVFDLIENGDALRQRL
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SDNADLFRTEMTKLGFTLAGEGHPIIPVMLGDAKLAQDMAGLMLKKGIYVIGFSFPVVPK
CCCHHHHHHHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCCCC
GQARIRTQMSAAHSRADVKRAIAAFAEAGRELGVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
TSPFLSHLRAEISALKDAGLYKSERVISSKQAGEIAISTGERVLNFCANNYLGLADNEE
CCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCC
LAVAGKQALDRYGYGMASVRFICGTQEEHKQLEARISAFLGMEDTILYSSCFDANGGLFE
HHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCHHEEEHHCCCCCCCHHH
TLLSEEDAIISDALNHASIIDGVRLSKAKRFRYANNDMAALEEELKKAEGSRFKLIATDG
HHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECC
VFSMDGIIANLGGVCDLAEKYGAMVMVDDSHAVGFVGKNGRGSPEHCGVEGRIDIITGTL
CEEHHHHHHHHCHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECHH
GKALGGASGGYTAAKAEVVEWLRQRSRPYLFSNTLAPVIAAASLKVFDLIENGDALRQRL
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SDNADLFRTEMTKLGFTLAGEGHPIIPVMLGDAKLAQDMAGLMLKKGIYVIGFSFPVVPK
CCCHHHHHHHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCCCC
GQARIRTQMSAAHSRADVKRAIAAFAEAGRELGVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]