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The map label for this gene is murF [H]

Identifier: 209550178

GI number: 209550178

Start: 2638613

End: 2640046

Strand: Reverse

Name: murF [H]

Synonym: Rleg2_2598

Alternate gene names: 209550178

Gene position: 2640046-2638613 (Counterclockwise)

Preceding gene: 209550179

Following gene: 209550177

Centisome position: 58.18

GC content: 62.34

Gene sequence:

>1434_bases
TTGAGCTGGCTCTGGACGACCGAAGATATGATCGCGGCGATGGCGGGGCGCCCCTTCGGCACTCTGCCCGAAGGCATCAC
CGGCATTTCCATCGACAGCCGCTCGATTGCTCCAGGCGAGGCCTTCTTCGCGATCAAGGGCGACCGTGTCGACGGTCACG
ACTACGCATCGATGGCGATGGCGAACGGCGCCTCCCTTCTCGTCGTCAGTGAGGCGAGGCTTCCCGCCATGGGCCGCCTG
ACGGTGCCGATGATCGTCGTGGAAGACGTGCTCGCCGCACTCGGCCGGCTCGGCCTTGCCTCGCGCCAGCGCTCCAAGGC
CCGGATCATCGCGGTGACGGGATCTGTCGGAAAGACGACCACCAAGGAAATGCTGCGGCACGTGCTGTCGCCCTCCGGCA
AGGTGCATGCCTCCGTCGCCTCCTTCAACAATCATTGGGGCGTGCCGCTGACGCTGGCGCGCATGCCTGACGATACGGAT
TACGGTGTCTTCGAAGTCGGCATGAACCATCCCGGCGAAATCCGGCCGCTGGTCGAGATGATCCGTCCCCATGTCGCCAT
CATCACGACCATCGCGCCGGCCCATCTCGGTAATTTCAAGAACATCAAGGAGATCGCCGCCGCCAAAGCGGAGATCTTCG
AAGGGCTGGAACCGGGCGGCCATGTCGTGCTCAACCGCGACAATGACCAGTTCAACTTCCTCGACCGAACCGCACAGTCG
CTTGGCATCGAGCATATTCATTCCTTCGGCCAGCATGCCAAGGCCGAATTCCGCCTCGCCGAATTCAACGGCGCAGATGA
GAATTCGACGCTGTGGCTGACGATCGGCGGTGAGACGCTGGAAGTCGCGATCGGCGCGCCCGGCCGCCATATCGCCGAGA
ATGCGCTTGCAGCGCTCGGGGTCGTCAGGATCGTCGGCGCCGACATGGAAAAGGCGATCGCAGCGCTGGCGACGCTGAAG
CCGGAAAAGGGCAGGGGCAAGCGCCACCGGCTTTCCATCGGCAGCGGCAGCTTCACGCTGATCGACGAAAGCTACAACGC
CAATCCGGCTTCGATGCGGGCGGCGATCGCACTGCTGGCGGCCTCCGAGCCGACCAGTCGCGGACGCCGTATTGCCGTGC
TGGGCGACATGCTTGAAATGGGCGAGTATGCGCAGAAGGTCCATACCGATCTCGTAGTGCCGCTGCTTGCAGCCGGCATC
GAACATGTCTGGCTTGCCGGCGCAGAGATGGCTGCGCTCAAGGAATCACTGCCGGAAAGCGTCCATGTCGAATATCGCGA
GAACACCGGCGAATTGACGGATTATGTATTGAACTCGGTCGCGCCAGGCGACGTGTTGATGGTGAAATCGTCTCTGGGCA
TCGGTTTCGGCAAGATCGTTGCCGCGCTCCTTGACAAGTTCCCGCCATTTTCCGACACGCAACGCGAATTTTGA

Upstream 100 bases:

>100_bases
TCGCCGGCAAAGGGCATGAGGAAGGGCAGACGATCGGCAGCGTCACCCTGCCGTTCTCCGATCATGCGGAGGTGCGCAAG
GCCTTGGAGGAACTGCAATC

Downstream 100 bases:

>100_bases
TCGGGTAAATAAGGGGCCCTGAATGCTGATCTGGCTTGTCGAACTGTCGGAATATTTCAAATTTCTGAACCTGTTCAGAT
ATATTACCTTCCGTACAGGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSWLWTTEDMIAAMAGRPFGTLPEGITGISIDSRSIAPGEAFFAIKGDRVDGHDYASMAMANGASLLVVSEARLPAMGRL
TVPMIVVEDVLAALGRLGLASRQRSKARIIAVTGSVGKTTTKEMLRHVLSPSGKVHASVASFNNHWGVPLTLARMPDDTD
YGVFEVGMNHPGEIRPLVEMIRPHVAIITTIAPAHLGNFKNIKEIAAAKAEIFEGLEPGGHVVLNRDNDQFNFLDRTAQS
LGIEHIHSFGQHAKAEFRLAEFNGADENSTLWLTIGGETLEVAIGAPGRHIAENALAALGVVRIVGADMEKAIAALATLK
PEKGRGKRHRLSIGSGSFTLIDESYNANPASMRAAIALLAASEPTSRGRRIAVLGDMLEMGEYAQKVHTDLVVPLLAAGI
EHVWLAGAEMAALKESLPESVHVEYRENTGELTDYVLNSVAPGDVLMVKSSLGIGFGKIVAALLDKFPPFSDTQREF

Sequences:

>Translated_477_residues
MSWLWTTEDMIAAMAGRPFGTLPEGITGISIDSRSIAPGEAFFAIKGDRVDGHDYASMAMANGASLLVVSEARLPAMGRL
TVPMIVVEDVLAALGRLGLASRQRSKARIIAVTGSVGKTTTKEMLRHVLSPSGKVHASVASFNNHWGVPLTLARMPDDTD
YGVFEVGMNHPGEIRPLVEMIRPHVAIITTIAPAHLGNFKNIKEIAAAKAEIFEGLEPGGHVVLNRDNDQFNFLDRTAQS
LGIEHIHSFGQHAKAEFRLAEFNGADENSTLWLTIGGETLEVAIGAPGRHIAENALAALGVVRIVGADMEKAIAALATLK
PEKGRGKRHRLSIGSGSFTLIDESYNANPASMRAAIALLAASEPTSRGRRIAVLGDMLEMGEYAQKVHTDLVVPLLAAGI
EHVWLAGAEMAALKESLPESVHVEYRENTGELTDYVLNSVAPGDVLMVKSSLGIGFGKIVAALLDKFPPFSDTQREF
>Mature_476_residues
SWLWTTEDMIAAMAGRPFGTLPEGITGISIDSRSIAPGEAFFAIKGDRVDGHDYASMAMANGASLLVVSEARLPAMGRLT
VPMIVVEDVLAALGRLGLASRQRSKARIIAVTGSVGKTTTKEMLRHVLSPSGKVHASVASFNNHWGVPLTLARMPDDTDY
GVFEVGMNHPGEIRPLVEMIRPHVAIITTIAPAHLGNFKNIKEIAAAKAEIFEGLEPGGHVVLNRDNDQFNFLDRTAQSL
GIEHIHSFGQHAKAEFRLAEFNGADENSTLWLTIGGETLEVAIGAPGRHIAENALAALGVVRIVGADMEKAIAALATLKP
EKGRGKRHRLSIGSGSFTLIDESYNANPASMRAAIALLAASEPTSRGRRIAVLGDMLEMGEYAQKVHTDLVVPLLAAGIE
HVWLAGAEMAALKESLPESVHVEYRENTGELTDYVLNSVAPGDVLMVKSSLGIGFGKIVAALLDKFPPFSDTQREF

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=391, Percent_Identity=36.0613810741688, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51042; Mature: 50911

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWLWTTEDMIAAMAGRPFGTLPEGITGISIDSRSIAPGEAFFAIKGDRVDGHDYASMAM
CCCEECHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHH
ANGASLLVVSEARLPAMGRLTVPMIVVEDVLAALGRLGLASRQRSKARIIAVTGSVGKTT
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEECCCCCHH
TKEMLRHVLSPSGKVHASVASFNNHWGVPLTLARMPDDTDYGVFEVGMNHPGEIRPLVEM
HHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHH
IRPHVAIITTIAPAHLGNFKNIKEIAAAKAEIFEGLEPGGHVVLNRDNDQFNFLDRTAQS
HCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
LGIEHIHSFGQHAKAEFRLAEFNGADENSTLWLTIGGETLEVAIGAPGRHIAENALAALG
HCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHH
VVRIVGADMEKAIAALATLKPEKGRGKRHRLSIGSGSFTLIDESYNANPASMRAAIALLA
HHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHC
ASEPTSRGRRIAVLGDMLEMGEYAQKVHTDLVVPLLAAGIEHVWLAGAEMAALKESLPES
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHCCCC
VHVEYRENTGELTDYVLNSVAPGDVLMVKSSLGIGFGKIVAALLDKFPPFSDTQREF
EEEEECCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SWLWTTEDMIAAMAGRPFGTLPEGITGISIDSRSIAPGEAFFAIKGDRVDGHDYASMAM
CCEECHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHH
ANGASLLVVSEARLPAMGRLTVPMIVVEDVLAALGRLGLASRQRSKARIIAVTGSVGKTT
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEECCCCCHH
TKEMLRHVLSPSGKVHASVASFNNHWGVPLTLARMPDDTDYGVFEVGMNHPGEIRPLVEM
HHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHH
IRPHVAIITTIAPAHLGNFKNIKEIAAAKAEIFEGLEPGGHVVLNRDNDQFNFLDRTAQS
HCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
LGIEHIHSFGQHAKAEFRLAEFNGADENSTLWLTIGGETLEVAIGAPGRHIAENALAALG
HCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHH
VVRIVGADMEKAIAALATLKPEKGRGKRHRLSIGSGSFTLIDESYNANPASMRAAIALLA
HHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHC
ASEPTSRGRRIAVLGDMLEMGEYAQKVHTDLVVPLLAAGIEHVWLAGAEMAALKESLPES
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHCCCC
VHVEYRENTGELTDYVLNSVAPGDVLMVKSSLGIGFGKIVAALLDKFPPFSDTQREF
EEEEECCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]