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The map label for this gene is murE [H]

Identifier: 209550179

GI number: 209550179

Start: 2640043

End: 2641560

Strand: Reverse

Name: murE [H]

Synonym: Rleg2_2599

Alternate gene names: 209550179

Gene position: 2641560-2640043 (Counterclockwise)

Preceding gene: 209550180

Following gene: 209550178

Centisome position: 58.21

GC content: 64.56

Gene sequence:

>1518_bases
ATGAATATCGACGCCGATTCGCGAGGGGTGGACCGGCTTCAAGAGAGAAAAGTGCATTCGATGAAATTGCGAGACCTGGC
CGGAGATCAGTTTCCGGAAATTGAAGCACAGCTCGAAGGCCCGGCAGGCTTGCTTGATATTTCAGGCCTGTCTTCGGATA
GCCGCCATGTGGCGCCGGGCAATGCTTTCGTTGCTGTCGCCGGCACCAAGGCGGACGGCGCGGGCTTCATCGCCGATGCT
GCCGGCCGGGGTGCCGCCGTTGCGATTGCTTCCCAGGCCGTCGAGACTTCCATCCCGGTGCTTGCGGTCAAGGAGCCGCG
CCGTTTCCTTTCGATCGCCGCCTCGCGCTTCTTCGGCAGGCAGCCGGAAACCATGGTCGCCGTCACCGGCACGGCGGGCA
AGACCTCTGTTGCCTCCTTCACCCGACAGATCTGGGCGCATGCGGGCCATGCGGCGGCGATGATCGGCACGACCGGTGTC
GTTTCGCCGACGCGCAACGAATATGGTGCGCTGACGACGCCCGATCCGGTGTCGCTGCACAAGCTGCTGGCCGAACTTGC
CGGCGAAGGCGTGACGCATGCGGCGATGGAGGCTTCCAGCCACGGCCTCGACCAGAGCCGGCTCGACGGCGTCAAGCTTG
CCGCCGCTGCCTTCACCAATCTCGGCCGCGACCACATGGATTATCATCCGACTGTCGAAGCCTATATGGCCGCCAAGATG
CGGCTTTTCCAGCTGCTGCCGAAGGGCGCGCCTGCGGTGATCTTCGCCGACGATCCGTGGTCGGCGCAGGCGATCAAAGC
TGCCGCCGATGCCGGTCACGATGTGCGCACCGTCGGGCGCAAGGGCGATTACCTTACGTTGAAGCGTGTCGAGCACTTCC
GCCACAAGCAGACCGCCGAGATCCATATCGACGGCGAGATCTTCGAGGTGGATATTCCGCTGGCCGGCGATTTCCAGGTG
GCCAACGCCCTGGTCGCGGCAGGTCTTGCCATGTCGACCGGAGTCGAGCCGAAAGTTGCGATGGCCGCGCTCGAGAAACT
CGTCGGCGCGTCCGGCCGTCTCGAACTCGTCGGCCATACGAAAGACGGCGCGCTCGCCTATGTCGACTATGCCCATAAGC
CGGACGCGCTGGAAAACGTGCTGGGCTCGGTCCGGCCCTTCACCACCGGCCGCGTCGTCGTGGTCTTCGGCTGCGGCGGC
GACCGTGATCGCGGCAAACGGCCGATCATGGGCGAAATCGCCTGCCGACTTGCCGACGTCGTCATCGTCACCGACGATAA
TCCGCGCTCGGAGGAGCCGGCCGCAATCCGGGCGGAGATCATGGCGGCAGCATCCGGCGCCTCGGAAATTGCCGATCGCG
CGAAGGCGATCCGCGAGGCTGTGGGCATGTTGCGATCCGGCGATACGCTGATCGTCGCCGGCAAAGGGCATGAGGAAGGG
CAGACGATCGGCAGCGTCACCCTGCCGTTCTCCGATCATGCGGAGGTGCGCAAGGCCTTGGAGGAACTGCAATCTTGA

Upstream 100 bases:

>100_bases
GATCGCCCGCGAGATCATCAGCCGCGCAGCTCCCATTCTCGGCATCGAGCCGAAATTCGGGGAGGGCGGATCGGCCTTGC
TGGTGTCTTATTGAGTGTGA

Downstream 100 bases:

>100_bases
GCTGGCTCTGGACGACCGAAGATATGATCGCGGCGATGGCGGGGCGCCCCTTCGGCACTCTGCCCGAAGGCATCACCGGC
ATTTCCATCGACAGCCGCTC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNIDADSRGVDRLQERKVHSMKLRDLAGDQFPEIEAQLEGPAGLLDISGLSSDSRHVAPGNAFVAVAGTKADGAGFIADA
AGRGAAVAIASQAVETSIPVLAVKEPRRFLSIAASRFFGRQPETMVAVTGTAGKTSVASFTRQIWAHAGHAAAMIGTTGV
VSPTRNEYGALTTPDPVSLHKLLAELAGEGVTHAAMEASSHGLDQSRLDGVKLAAAAFTNLGRDHMDYHPTVEAYMAAKM
RLFQLLPKGAPAVIFADDPWSAQAIKAAADAGHDVRTVGRKGDYLTLKRVEHFRHKQTAEIHIDGEIFEVDIPLAGDFQV
ANALVAAGLAMSTGVEPKVAMAALEKLVGASGRLELVGHTKDGALAYVDYAHKPDALENVLGSVRPFTTGRVVVVFGCGG
DRDRGKRPIMGEIACRLADVVIVTDDNPRSEEPAAIRAEIMAAASGASEIADRAKAIREAVGMLRSGDTLIVAGKGHEEG
QTIGSVTLPFSDHAEVRKALEELQS

Sequences:

>Translated_505_residues
MNIDADSRGVDRLQERKVHSMKLRDLAGDQFPEIEAQLEGPAGLLDISGLSSDSRHVAPGNAFVAVAGTKADGAGFIADA
AGRGAAVAIASQAVETSIPVLAVKEPRRFLSIAASRFFGRQPETMVAVTGTAGKTSVASFTRQIWAHAGHAAAMIGTTGV
VSPTRNEYGALTTPDPVSLHKLLAELAGEGVTHAAMEASSHGLDQSRLDGVKLAAAAFTNLGRDHMDYHPTVEAYMAAKM
RLFQLLPKGAPAVIFADDPWSAQAIKAAADAGHDVRTVGRKGDYLTLKRVEHFRHKQTAEIHIDGEIFEVDIPLAGDFQV
ANALVAAGLAMSTGVEPKVAMAALEKLVGASGRLELVGHTKDGALAYVDYAHKPDALENVLGSVRPFTTGRVVVVFGCGG
DRDRGKRPIMGEIACRLADVVIVTDDNPRSEEPAAIRAEIMAAASGASEIADRAKAIREAVGMLRSGDTLIVAGKGHEEG
QTIGSVTLPFSDHAEVRKALEELQS
>Mature_505_residues
MNIDADSRGVDRLQERKVHSMKLRDLAGDQFPEIEAQLEGPAGLLDISGLSSDSRHVAPGNAFVAVAGTKADGAGFIADA
AGRGAAVAIASQAVETSIPVLAVKEPRRFLSIAASRFFGRQPETMVAVTGTAGKTSVASFTRQIWAHAGHAAAMIGTTGV
VSPTRNEYGALTTPDPVSLHKLLAELAGEGVTHAAMEASSHGLDQSRLDGVKLAAAAFTNLGRDHMDYHPTVEAYMAAKM
RLFQLLPKGAPAVIFADDPWSAQAIKAAADAGHDVRTVGRKGDYLTLKRVEHFRHKQTAEIHIDGEIFEVDIPLAGDFQV
ANALVAAGLAMSTGVEPKVAMAALEKLVGASGRLELVGHTKDGALAYVDYAHKPDALENVLGSVRPFTTGRVVVVFGCGG
DRDRGKRPIMGEIACRLADVVIVTDDNPRSEEPAAIRAEIMAAASGASEIADRAKAIREAVGMLRSGDTLIVAGKGHEEG
QTIGSVTLPFSDHAEVRKALEELQS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=508, Percent_Identity=37.5984251968504, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53045; Mature: 53045

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDADSRGVDRLQERKVHSMKLRDLAGDQFPEIEAQLEGPAGLLDISGLSSDSRHVAPG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCCCCCEECCC
NAFVAVAGTKADGAGFIADAAGRGAAVAIASQAVETSIPVLAVKEPRRFLSIAASRFFGR
CEEEEEEECCCCCCCEEEECCCCCEEEEEHHHHHHHCCCEEEECCHHHHHHHHHHHHCCC
QPETMVAVTGTAGKTSVASFTRQIWAHAGHAAAMIGTTGVVSPTRNEYGALTTPDPVSLH
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHH
KLLAELAGEGVTHAAMEASSHGLDQSRLDGVKLAAAAFTNLGRDHMDYHPTVEAYMAAKM
HHHHHHHCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RLFQLLPKGAPAVIFADDPWSAQAIKAAADAGHDVRTVGRKGDYLTLKRVEHFRHKQTAE
HHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHCCCCCEEEHHHHHHHHCCCCEE
IHIDGEIFEVDIPLAGDFQVANALVAAGLAMSTGVEPKVAMAALEKLVGASGRLELVGHT
EEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEC
KDGALAYVDYAHKPDALENVLGSVRPFTTGRVVVVFGCGGDRDRGKRPIMGEIACRLADV
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEEE
VIVTDDNPRSEEPAAIRAEIMAAASGASEIADRAKAIREAVGMLRSGDTLIVAGKGHEEG
EEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
QTIGSVTLPFSDHAEVRKALEELQS
CCCCEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNIDADSRGVDRLQERKVHSMKLRDLAGDQFPEIEAQLEGPAGLLDISGLSSDSRHVAPG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCCCCCEECCC
NAFVAVAGTKADGAGFIADAAGRGAAVAIASQAVETSIPVLAVKEPRRFLSIAASRFFGR
CEEEEEEECCCCCCCEEEECCCCCEEEEEHHHHHHHCCCEEEECCHHHHHHHHHHHHCCC
QPETMVAVTGTAGKTSVASFTRQIWAHAGHAAAMIGTTGVVSPTRNEYGALTTPDPVSLH
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHH
KLLAELAGEGVTHAAMEASSHGLDQSRLDGVKLAAAAFTNLGRDHMDYHPTVEAYMAAKM
HHHHHHHCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RLFQLLPKGAPAVIFADDPWSAQAIKAAADAGHDVRTVGRKGDYLTLKRVEHFRHKQTAE
HHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHCCCCCEEEHHHHHHHHCCCCEE
IHIDGEIFEVDIPLAGDFQVANALVAAGLAMSTGVEPKVAMAALEKLVGASGRLELVGHT
EEECCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEC
KDGALAYVDYAHKPDALENVLGSVRPFTTGRVVVVFGCGGDRDRGKRPIMGEIACRLADV
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEEE
VIVTDDNPRSEEPAAIRAEIMAAASGASEIADRAKAIREAVGMLRSGDTLIVAGKGHEEG
EEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
QTIGSVTLPFSDHAEVRKALEELQS
CCCCEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]