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The map label for this gene is pdxJ [H]

Identifier: 209550095

GI number: 209550095

Start: 2545870

End: 2546622

Strand: Reverse

Name: pdxJ [H]

Synonym: Rleg2_2512

Alternate gene names: 209550095

Gene position: 2546622-2545870 (Counterclockwise)

Preceding gene: 209550097

Following gene: 209550093

Centisome position: 56.12

GC content: 66.4

Gene sequence:

>753_bases
ATGCCCGCCAAGCTCTCCGTGAACCTCAACGCCGTCGCCATGCTGCGCAACCGACGCGACCTGCCCTGGCCGAGCGTCGA
AGCGCTGGGGCGAATCGCCCTTGCGTCAGGCGCGAGCGGCCTGACGGTGCATCCGCGCCCCGACCAGCGCCATATTCGCT
TTTCCGACCTGCCCGTCATCCGCAATCTGATCGATGACGAATTCCCGAACGCCGAGTTTAATATAGAGGGCTATCCGACT
GAGGAGTTCTTCGAGCTCTGCGCCGGGGCGGCGCCGGAGCAGGTGACGCTGGTGCCCGACGATCCCGCGCAGGCGACCTC
GGATCACGGCTGGGATTTCCGCACGCACCGGGCATTTCTGACCGATGCCGTCGCACGGCTGAAGACGATGGGATGCCGGG
TCTCGCTGTTTGCCGACGGCGACGGGGATGCTGAAGCCGTCGAGATCGCCAAGGCCGTCGGCGCCGACCGGATCGAACTT
TATACCGGTCCCTATGGCGGCTGTTACGACGCGCCGGAACGGGCGGTCCCGATCCTCGAAGCGCTCGGACGGACGGCGGA
TGCGTCGCTGGCGATCGGCCTTGCCGTCAATGCCGGCCACGACCTGACGGTCGCAAACCTGCCTGATCTGGTGAAGCGCA
TCCCGGCGCTTGCCGAAGTCTCGATCGGCCATGGACTGACCGCCGATGCACTGGAGTATGGCATGGCCGAGACGGTACGG
CGGTTCTGCCGGGCGTGCGGGCAGGCGGTTTGA

Upstream 100 bases:

>100_bases
AACGTCCGGCATCGGATCCATCTGCGAATCTCGTTGGCCATTGCAGGGCACTTGGCTTAAAAGCAGGGCCTCCTGTTTCC
GTGACCGATGGATGCCAGCC

Downstream 100 bases:

>100_bases
GTGGGATCTTCTTCGAGGCGGTAAGAAAGGGTCTTTTGCGCCCAAGGACTTGGGCGCGCTGGATTCCTGTGACAGGCACA
GGAATGAGGGTGGAGAGGTA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPAKLSVNLNAVAMLRNRRDLPWPSVEALGRIALASGASGLTVHPRPDQRHIRFSDLPVIRNLIDDEFPNAEFNIEGYPT
EEFFELCAGAAPEQVTLVPDDPAQATSDHGWDFRTHRAFLTDAVARLKTMGCRVSLFADGDGDAEAVEIAKAVGADRIEL
YTGPYGGCYDAPERAVPILEALGRTADASLAIGLAVNAGHDLTVANLPDLVKRIPALAEVSIGHGLTADALEYGMAETVR
RFCRACGQAV

Sequences:

>Translated_250_residues
MPAKLSVNLNAVAMLRNRRDLPWPSVEALGRIALASGASGLTVHPRPDQRHIRFSDLPVIRNLIDDEFPNAEFNIEGYPT
EEFFELCAGAAPEQVTLVPDDPAQATSDHGWDFRTHRAFLTDAVARLKTMGCRVSLFADGDGDAEAVEIAKAVGADRIEL
YTGPYGGCYDAPERAVPILEALGRTADASLAIGLAVNAGHDLTVANLPDLVKRIPALAEVSIGHGLTADALEYGMAETVR
RFCRACGQAV
>Mature_249_residues
PAKLSVNLNAVAMLRNRRDLPWPSVEALGRIALASGASGLTVHPRPDQRHIRFSDLPVIRNLIDDEFPNAEFNIEGYPTE
EFFELCAGAAPEQVTLVPDDPAQATSDHGWDFRTHRAFLTDAVARLKTMGCRVSLFADGDGDAEAVEIAKAVGADRIELY
TGPYGGCYDAPERAVPILEALGRTADASLAIGLAVNAGHDLTVANLPDLVKRIPALAEVSIGHGLTADALEYGMAETVRR
FCRACGQAV

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=240, Percent_Identity=34.5833333333333, Blast_Score=126, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 26804; Mature: 26672

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLSVNLNAVAMLRNRRDLPWPSVEALGRIALASGASGLTVHPRPDQRHIRFSDLPVI
CCCEEECCHHHHEEECCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCEEECCCHHH
RNLIDDEFPNAEFNIEGYPTEEFFELCAGAAPEQVTLVPDDPAQATSDHGWDFRTHRAFL
HHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHH
TDAVARLKTMGCRVSLFADGDGDAEAVEIAKAVGADRIELYTGPYGGCYDAPERAVPILE
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHH
ALGRTADASLAIGLAVNAGHDLTVANLPDLVKRIPALAEVSIGHGLTADALEYGMAETVR
HHCCCCCCEEEEEEEEECCCCEEECCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHH
RFCRACGQAV
HHHHHHCCCC
>Mature Secondary Structure 
PAKLSVNLNAVAMLRNRRDLPWPSVEALGRIALASGASGLTVHPRPDQRHIRFSDLPVI
CCEEECCHHHHEEECCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCEEECCCHHH
RNLIDDEFPNAEFNIEGYPTEEFFELCAGAAPEQVTLVPDDPAQATSDHGWDFRTHRAFL
HHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHH
TDAVARLKTMGCRVSLFADGDGDAEAVEIAKAVGADRIELYTGPYGGCYDAPERAVPILE
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHH
ALGRTADASLAIGLAVNAGHDLTVANLPDLVKRIPALAEVSIGHGLTADALEYGMAETVR
HHCCCCCCEEEEEEEEECCCCEEECCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHH
RFCRACGQAV
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]