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The map label for this gene is cobA [H]

Identifier: 209549717

GI number: 209549717

Start: 2182332

End: 2183171

Strand: Reverse

Name: cobA [H]

Synonym: Rleg2_2124

Alternate gene names: 209549717

Gene position: 2183171-2182332 (Counterclockwise)

Preceding gene: 209549718

Following gene: 209549716

Centisome position: 48.11

GC content: 67.14

Gene sequence:

>840_bases
ATGTTGAATAGCCAACTTTCCCATCTTCCGGCGCTGGAGCCGGGCAGCGTCTGGCTTGTCGGGGCCGGCCCCGGTGATCC
CGGGCTGTTGACACTGCTGGCCGCCAAGGGGCTTTCCGAGGCTGATGTCATCGTGCACGACGCGCTCGTCAACGAGGAAT
GCCTGGCGCTGGCGCGGCCGGAGGCGGTGGTGGAATTTGCGGGAAAACGCGGCGGCAAGCCTTCGGCGAAACAGCGCGAC
ATCTCGCTGCGGCTGGTAGAACTGGCGCGGGCCGGCAAGCGGGTGCTGAGATTGAAGGGCGGTGACCCCTTCGTCTTTGG
CCGTGGCGGCGAGGAAGCGCTGACGCTGGTCGAGCACAACATCCCCTTCCGCATCGTGCCCGGCATCACTGCCGGTATCG
GCGGGCTTGCCTATGCCGGCATCCCGGTGACGCATCGCGACATCAACCATGCCGTCACCTTCCTGACCGGCCATGATTCT
TCCGGCATCGTGCCCGACAGGATCAACTGGGAGGCGATCGGCCGGGGCTCGCCCGTCATCGTCATGTATATGGCGATGAA
ACATATCGCAGAGATCAGCGCCCGGTTGATCGCCGCCGGCCGGCTGCCCTCCGAACCCGTCGCCTTCGTCTGCAACGCCG
CCACCGGCCGGCAGCAGGTGCTGGAAACGACGCTTGGCGACGCGCCCGAGACGGTCGCCGCCTCCGGGCTCGAACCGCCG
GCGGTAGTCGTCGTCGGCGAGGTGGTGCGGCTCAGAGCCTCGCTCGACTGGCTCGGCGCACTGGCGGGCAGGCGCCTGCA
GCCCGATCCATTTCGCCGGTCCGGCAAGGTGCTGATATGA

Upstream 100 bases:

>100_bases
TCCGCTTTCTCGCAAAGTGAGTCATGGTCATCTCAGCAACTTCGAGTGAGGCGGGGCAGGCGGCGTTGACCTTGCTCCTT
GCTGGGCCTAGATCAACAAA

Downstream 100 bases:

>100_bases
GCGGCCTCCTGATCGCAGCCCCTTCCTCCGGCGCCGGCAAGACGACGGTGACGCTCGGGATGCTGAGGGCGTTGCGCCGG
CGCGGCGTCGCGGTGGCACC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLNSQLSHLPALEPGSVWLVGAGPGDPGLLTLLAAKGLSEADVIVHDALVNEECLALARPEAVVEFAGKRGGKPSAKQRD
ISLRLVELARAGKRVLRLKGGDPFVFGRGGEEALTLVEHNIPFRIVPGITAGIGGLAYAGIPVTHRDINHAVTFLTGHDS
SGIVPDRINWEAIGRGSPVIVMYMAMKHIAEISARLIAAGRLPSEPVAFVCNAATGRQQVLETTLGDAPETVAASGLEPP
AVVVVGEVVRLRASLDWLGALAGRRLQPDPFRRSGKVLI

Sequences:

>Translated_279_residues
MLNSQLSHLPALEPGSVWLVGAGPGDPGLLTLLAAKGLSEADVIVHDALVNEECLALARPEAVVEFAGKRGGKPSAKQRD
ISLRLVELARAGKRVLRLKGGDPFVFGRGGEEALTLVEHNIPFRIVPGITAGIGGLAYAGIPVTHRDINHAVTFLTGHDS
SGIVPDRINWEAIGRGSPVIVMYMAMKHIAEISARLIAAGRLPSEPVAFVCNAATGRQQVLETTLGDAPETVAASGLEPP
AVVVVGEVVRLRASLDWLGALAGRRLQPDPFRRSGKVLI
>Mature_279_residues
MLNSQLSHLPALEPGSVWLVGAGPGDPGLLTLLAAKGLSEADVIVHDALVNEECLALARPEAVVEFAGKRGGKPSAKQRD
ISLRLVELARAGKRVLRLKGGDPFVFGRGGEEALTLVEHNIPFRIVPGITAGIGGLAYAGIPVTHRDINHAVTFLTGHDS
SGIVPDRINWEAIGRGSPVIVMYMAMKHIAEISARLIAAGRLPSEPVAFVCNAATGRQQVLETTLGDAPETVAASGLEPP
AVVVVGEVVRLRASLDWLGALAGRRLQPDPFRRSGKVLI

Specific function: Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=257, Percent_Identity=43.9688715953307, Blast_Score=200, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6322922, Length=266, Percent_Identity=31.9548872180451, Blast_Score=115, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 29354; Mature: 29354

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSQLSHLPALEPGSVWLVGAGPGDPGLLTLLAAKGLSEADVIVHDALVNEECLALARP
CCCCCCHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHCCC
EAVVEFAGKRGGKPSAKQRDISLRLVELARAGKRVLRLKGGDPFVFGRGGEEALTLVEHN
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCEEEEEECC
IPFRIVPGITAGIGGLAYAGIPVTHRDINHAVTFLTGHDSSGIVPDRINWEAIGRGSPVI
CCEEECCCCHHCCCHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEE
VMYMAMKHIAEISARLIAAGRLPSEPVAFVCNAATGRQQVLETTLGDAPETVAASGLEPP
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHCCCCCC
AVVVVGEVVRLRASLDWLGALAGRRLQPDPFRRSGKVLI
EEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLNSQLSHLPALEPGSVWLVGAGPGDPGLLTLLAAKGLSEADVIVHDALVNEECLALARP
CCCCCCHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHCCC
EAVVEFAGKRGGKPSAKQRDISLRLVELARAGKRVLRLKGGDPFVFGRGGEEALTLVEHN
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCEEEEEECC
IPFRIVPGITAGIGGLAYAGIPVTHRDINHAVTFLTGHDSSGIVPDRINWEAIGRGSPVI
CCEEECCCCHHCCCHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEE
VMYMAMKHIAEISARLIAAGRLPSEPVAFVCNAATGRQQVLETTLGDAPETVAASGLEPP
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHCCCCCC
AVVVVGEVVRLRASLDWLGALAGRRLQPDPFRRSGKVLI
EEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211520; 2546914 [H]