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The map label for this gene is cobB [H]

Identifier: 209549716

GI number: 209549716

Start: 2181028

End: 2182335

Strand: Reverse

Name: cobB [H]

Synonym: Rleg2_2123

Alternate gene names: 209549716

Gene position: 2182335-2181028 (Counterclockwise)

Preceding gene: 209549717

Following gene: 209549715

Centisome position: 48.09

GC content: 65.75

Gene sequence:

>1308_bases
ATGAGCGGCCTCCTGATCGCAGCCCCTTCCTCCGGCGCCGGCAAGACGACGGTGACGCTCGGGATGCTGAGGGCGTTGCG
CCGGCGCGGCGTCGCGGTGGCACCCGGCAAGGCCGGGCCGGATTACATCGATCCCGCTTTCCATGCGGCGGCAAGCGGCA
CACCCTGCCTGAATTTCGATCCCTGGGCGATGCGCCCGGAACTGATCTCGGCCAATTCAGCCCTTCACCGCTTCGGTGAC
CGCATTTTGGTCATCGAGGCGATGATGGGGCTCTTCGACGGAGCGGCCGACGGAAAGGGAACCGCGGCCGATCTTGCCGC
CCAGCTCGGCCTTTCCGTGGTGCTGGTCGTCGACGCCTCGCGCATGTCGCAGTCGGTGGCGCCGCTGGTATCAGGTTTTG
CCGGCTTCCGCGCCGATGTCCGCGTTGCCGGCGTCATCCTCAACAAGGTCGGCAGCGAGCGGCATGAGGCGATGCTGCGC
CAGGCGCTCGAAGCGATCCGCATGCCTGTTATTGCCGTGATCGGCAGCGACAAGGCACTTGCCCTGCCGGAGCGCCATCT
CGGCCTCGTCCAGGCCGGCGAACATGCGACGCTTGAAAATTTCATCGACCATGCGGCGGACGCCGTTTCCGAGGAATGCA
ATTTCGAATTCCTGCTGCGCATTGCAAGGCAGGGGCTCAACCGGCCGTCTGCCGCCAATATCGACCGGCTGATGCCGATC
GGCGCCCGCATCGCCGTGGCGCGCGATGTCGCCTTTGCCTTTTCCTACGAGCATATGTTGCTCGGCTGGCGGCGCCGCGG
GGCTGATATTTCCTTCTTCTCGCCGCTTGCCGACGAGGTGCCGGCCGCCAATGCCGATGCGATCTATCTGCCGGGCGGTT
ATCCCGAGCTGCATGCCGGGCGGCTATCCCAAGCGCAGAATTTTCGCGCCGGCATGCTCGATGCGGCGGCGCGCGGCATC
AGGATCTATGGCGAGTGCGGCGGCTACATGGTGCTCGGCGACGGGCTGATCGATGCAGAGGGCACGCGCCACGAAATGCT
CGGGCTGCTGCCGGTCGTCACCAGCTATGCCGAGCGCCGGCGCCATCTCGGCTATCGCCGTGTCACGCCGCTCGACGGCG
CCTTCTTCGCGCGGACGATGACGGCGCATGAATTCCACTATTCCACCGTTGTCTCCGAGGGCGAGGGAAAGCGGCTGTTT
GCCGCACAGGACGCATTGGGAACCGATCTTGGTGCTGTCGGGCTGAGACGGGGGCAGGTGGCCGGTTCCTATATGCATCT
GATCGACCTTGCGAGAGCGGCCGCGTGA

Upstream 100 bases:

>100_bases
GCGAGGTGGTGCGGCTCAGAGCCTCGCTCGACTGGCTCGGCGCACTGGCGGGCAGGCGCCTGCAGCCCGATCCATTTCGC
CGGTCCGGCAAGGTGCTGAT

Downstream 100 bases:

>100_bases
GCGCGCCGATCGCCCATGGCGGCGGTATCACCGCTGCAGCCGCTGCTTTCGGCGGCAGGCCGGAAGACTGGCTCGACCTT
TCCACAGGAATCAACCCCTG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSGLLIAAPSSGAGKTTVTLGMLRALRRRGVAVAPGKAGPDYIDPAFHAAASGTPCLNFDPWAMRPELISANSALHRFGD
RILVIEAMMGLFDGAADGKGTAADLAAQLGLSVVLVVDASRMSQSVAPLVSGFAGFRADVRVAGVILNKVGSERHEAMLR
QALEAIRMPVIAVIGSDKALALPERHLGLVQAGEHATLENFIDHAADAVSEECNFEFLLRIARQGLNRPSAANIDRLMPI
GARIAVARDVAFAFSYEHMLLGWRRRGADISFFSPLADEVPAANADAIYLPGGYPELHAGRLSQAQNFRAGMLDAAARGI
RIYGECGGYMVLGDGLIDAEGTRHEMLGLLPVVTSYAERRRHLGYRRVTPLDGAFFARTMTAHEFHYSTVVSEGEGKRLF
AAQDALGTDLGAVGLRRGQVAGSYMHLIDLARAAA

Sequences:

>Translated_435_residues
MSGLLIAAPSSGAGKTTVTLGMLRALRRRGVAVAPGKAGPDYIDPAFHAAASGTPCLNFDPWAMRPELISANSALHRFGD
RILVIEAMMGLFDGAADGKGTAADLAAQLGLSVVLVVDASRMSQSVAPLVSGFAGFRADVRVAGVILNKVGSERHEAMLR
QALEAIRMPVIAVIGSDKALALPERHLGLVQAGEHATLENFIDHAADAVSEECNFEFLLRIARQGLNRPSAANIDRLMPI
GARIAVARDVAFAFSYEHMLLGWRRRGADISFFSPLADEVPAANADAIYLPGGYPELHAGRLSQAQNFRAGMLDAAARGI
RIYGECGGYMVLGDGLIDAEGTRHEMLGLLPVVTSYAERRRHLGYRRVTPLDGAFFARTMTAHEFHYSTVVSEGEGKRLF
AAQDALGTDLGAVGLRRGQVAGSYMHLIDLARAAA
>Mature_434_residues
SGLLIAAPSSGAGKTTVTLGMLRALRRRGVAVAPGKAGPDYIDPAFHAAASGTPCLNFDPWAMRPELISANSALHRFGDR
ILVIEAMMGLFDGAADGKGTAADLAAQLGLSVVLVVDASRMSQSVAPLVSGFAGFRADVRVAGVILNKVGSERHEAMLRQ
ALEAIRMPVIAVIGSDKALALPERHLGLVQAGEHATLENFIDHAADAVSEECNFEFLLRIARQGLNRPSAANIDRLMPIG
ARIAVARDVAFAFSYEHMLLGWRRRGADISFFSPLADEVPAANADAIYLPGGYPELHAGRLSQAQNFRAGMLDAAARGIR
IYGECGGYMVLGDGLIDAEGTRHEMLGLLPVVTSYAERRRHLGYRRVTPLDGAFFARTMTAHEFHYSTVVSEGEGKRLFA
AQDALGTDLGAVGLRRGQVAGSYMHLIDLARAAA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 46283; Mature: 46152

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLLIAAPSSGAGKTTVTLGMLRALRRRGVAVAPGKAGPDYIDPAFHAAASGTPCLNFD
CCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHCCCCCCCCCC
PWAMRPELISANSALHRFGDRILVIEAMMGLFDGAADGKGTAADLAAQLGLSVVLVVDAS
CCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEEEHH
RMSQSVAPLVSGFAGFRADVRVAGVILNKVGSERHEAMLRQALEAIRMPVIAVIGSDKAL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCEE
ALPERHLGLVQAGEHATLENFIDHAADAVSEECNFEFLLRIARQGLNRPSAANIDRLMPI
ECCHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCC
GARIAVARDVAFAFSYEHMLLGWRRRGADISFFSPLADEVPAANADAIYLPGGYPELHAG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCCCCEEEECCCCCCCCCH
RLSQAQNFRAGMLDAAARGIRIYGECGGYMVLGDGLIDAEGTRHEMLGLLPVVTSYAERR
HHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHH
RHLGYRRVTPLDGAFFARTMTAHEFHYSTVVSEGEGKRLFAAQDALGTDLGAVGLRRGQV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEEECHHHCCCHHHHCCCCCCH
AGSYMHLIDLARAAA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGLLIAAPSSGAGKTTVTLGMLRALRRRGVAVAPGKAGPDYIDPAFHAAASGTPCLNFD
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHCCCCCCCCCC
PWAMRPELISANSALHRFGDRILVIEAMMGLFDGAADGKGTAADLAAQLGLSVVLVVDAS
CCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEEEHH
RMSQSVAPLVSGFAGFRADVRVAGVILNKVGSERHEAMLRQALEAIRMPVIAVIGSDKAL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCEE
ALPERHLGLVQAGEHATLENFIDHAADAVSEECNFEFLLRIARQGLNRPSAANIDRLMPI
ECCHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCC
GARIAVARDVAFAFSYEHMLLGWRRRGADISFFSPLADEVPAANADAIYLPGGYPELHAG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCCCCEEEECCCCCCCCCH
RLSQAQNFRAGMLDAAARGIRIYGECGGYMVLGDGLIDAEGTRHEMLGLLPVVTSYAERR
HHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHH
RHLGYRRVTPLDGAFFARTMTAHEFHYSTVVSEGEGKRLFAAQDALGTDLGAVGLRRGQV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEEECHHHCCCHHHHCCCCCCH
AGSYMHLIDLARAAA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]