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The map label for this gene is narK [H]

Identifier: 209549056

GI number: 209549056

Start: 1478273

End: 1479514

Strand: Reverse

Name: narK [H]

Synonym: Rleg2_1453

Alternate gene names: 209549056

Gene position: 1479514-1478273 (Counterclockwise)

Preceding gene: 209549057

Following gene: 209549055

Centisome position: 32.6

GC content: 63.69

Gene sequence:

>1242_bases
ATGTCTGTCATCGAGAAAGCGCAGCCCATGTCCCCCGGCGAACCAGCCAAAGCATTGTGGATCTCCACGGTCGCCTTCAC
GCTCTGTTTTGCCGTCTGGACGATCTTTGCAATCATCGGCATCCGCATCAAGCAGGACCTCGGCCTGAACGAGGCGGAGT
TTGGCTTGCTGATCGGCACCCCGGTCCTGACCGGTTCGCTGGTGCGCATCGTCCTCGGCATCTGGACGGGGCGTTATGGC
GGGCGTCTCGTTTACACCCTCACCATGCTGGCCGCCGCATTGGCGACCTTCCTGCTCTCCTACGCCCAGACTTATACGCA
GATGCTGATCGCCGGCCTCGGCGTCGGGCTTGCCGGCGGCTCCTTCGCGGTCGGTGTCGCCTATGTCTCGCCGTTCTTCC
CGGCGGAAAAACAGGGCACGGCGCTCGGCATCTTCGGCGCCGGCAATGTCGGCGCGGCCGTGACCAAATTCGCAGCCCCC
TTCGTGCTGCTCGCCTGGGGCTGGCAGTCGGTTGCCGAGATCTGGGCCGTCTGTCTGGCGCTGATGGCCATCGTCTTCTG
GTTCACCACGTCAGATGATCCGGCCTTCCGTCTTCGCCGCGAGCGCGGCACCGCCTCCAAGAGCCTCGCTCAGGAATTCG
CGCCGCTGCAGAACGTTCAGGTCTGGCGCTTCTCGCTCTACTATTTCTTCGCCTTCGGCGGCTTCGTCGCCCTGTCCCTG
TGGCTGCCGCGCTATCTGGTCGGCGTCTACGGCTTCAACCTGGAAACTGCCGGCATGATTGCGGCCGCCTACTCCATCCC
GGGCAGCATCTTCCGCGCTTTCGGCGGCGTGCTGTCGGACAAGAAGGGCGCACGCAGCGTGATGTATGCGATGCTGGCCG
TCTCGGCCGTAGCCACTCTCATCCTGTCGCTGCCGGCGACGACCATCACGCCGGTCATCTTCATCGCCGTCATCTTCGTG
CTCGGCTTCTTCATGAGCCTCGGCAAGGCCGCCGTCTACAAGCACATTCCGGCCTATTACCCTGAAAGCGTCGGCGCCGT
CGGCGGCATCGTCGGCATGATGGGCGGTCTCGGTGGCTTCATCCTGCCGATCGCTTTCGGCCTCCTCAAGGACATGACCG
GCCTTTGGTCCAGCTGCTTCCTGCTGCTCTTCGCAATCGTCGCGATCTCGCTCGTCTGGATGCACCTGTCCGTCAAGCAA
TTGTCGCGCCAGGGACCCTCTGAGCCCGTGGCTGCAACCTGA

Upstream 100 bases:

>100_bases
TATTTCGCGCGCCTCGTGCATGCGCAGCCTGACCAGCGGCTTTTTGTTTTTAACCCTCCAGCAATGGATCGGCACGACCT
TGTCGCGCCACGGAGAAGCC

Downstream 100 bases:

>100_bases
TCTAAGGACTAGGAATTATGACAGAAAAACTCGTCATCATCGGCAATGGCATGGCGCCCGGGCGCATGCTGGAGCACCTC
TTCGAACGGGCGCCCGGACG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSVIEKAQPMSPGEPAKALWISTVAFTLCFAVWTIFAIIGIRIKQDLGLNEAEFGLLIGTPVLTGSLVRIVLGIWTGRYG
GRLVYTLTMLAAALATFLLSYAQTYTQMLIAGLGVGLAGGSFAVGVAYVSPFFPAEKQGTALGIFGAGNVGAAVTKFAAP
FVLLAWGWQSVAEIWAVCLALMAIVFWFTTSDDPAFRLRRERGTASKSLAQEFAPLQNVQVWRFSLYYFFAFGGFVALSL
WLPRYLVGVYGFNLETAGMIAAAYSIPGSIFRAFGGVLSDKKGARSVMYAMLAVSAVATLILSLPATTITPVIFIAVIFV
LGFFMSLGKAAVYKHIPAYYPESVGAVGGIVGMMGGLGGFILPIAFGLLKDMTGLWSSCFLLLFAIVAISLVWMHLSVKQ
LSRQGPSEPVAAT

Sequences:

>Translated_413_residues
MSVIEKAQPMSPGEPAKALWISTVAFTLCFAVWTIFAIIGIRIKQDLGLNEAEFGLLIGTPVLTGSLVRIVLGIWTGRYG
GRLVYTLTMLAAALATFLLSYAQTYTQMLIAGLGVGLAGGSFAVGVAYVSPFFPAEKQGTALGIFGAGNVGAAVTKFAAP
FVLLAWGWQSVAEIWAVCLALMAIVFWFTTSDDPAFRLRRERGTASKSLAQEFAPLQNVQVWRFSLYYFFAFGGFVALSL
WLPRYLVGVYGFNLETAGMIAAAYSIPGSIFRAFGGVLSDKKGARSVMYAMLAVSAVATLILSLPATTITPVIFIAVIFV
LGFFMSLGKAAVYKHIPAYYPESVGAVGGIVGMMGGLGGFILPIAFGLLKDMTGLWSSCFLLLFAIVAISLVWMHLSVKQ
LSRQGPSEPVAAT
>Mature_412_residues
SVIEKAQPMSPGEPAKALWISTVAFTLCFAVWTIFAIIGIRIKQDLGLNEAEFGLLIGTPVLTGSLVRIVLGIWTGRYGG
RLVYTLTMLAAALATFLLSYAQTYTQMLIAGLGVGLAGGSFAVGVAYVSPFFPAEKQGTALGIFGAGNVGAAVTKFAAPF
VLLAWGWQSVAEIWAVCLALMAIVFWFTTSDDPAFRLRRERGTASKSLAQEFAPLQNVQVWRFSLYYFFAFGGFVALSLW
LPRYLVGVYGFNLETAGMIAAAYSIPGSIFRAFGGVLSDKKGARSVMYAMLAVSAVATLILSLPATTITPVIFIAVIFVL
GFFMSLGKAAVYKHIPAYYPESVGAVGGIVGMMGGLGGFILPIAFGLLKDMTGLWSSCFLLLFAIVAISLVWMHLSVKQL
SRQGPSEPVAAT

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=444, Percent_Identity=25.9009009009009, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787475, Length=433, Percent_Identity=24.7113163972286, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44029; Mature: 43898

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIEKAQPMSPGEPAKALWISTVAFTLCFAVWTIFAIIGIRIKQDLGLNEAEFGLLIGT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECC
PVLTGSLVRIVLGIWTGRYGGRLVYTLTMLAAALATFLLSYAQTYTQMLIAGLGVGLAGG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SFAVGVAYVSPFFPAEKQGTALGIFGAGNVGAAVTKFAAPFVLLAWGWQSVAEIWAVCLA
HHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LMAIVFWFTTSDDPAFRLRRERGTASKSLAQEFAPLQNVQVWRFSLYYFFAFGGFVALSL
HHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
WLPRYLVGVYGFNLETAGMIAAAYSIPGSIFRAFGGVLSDKKGARSVMYAMLAVSAVATL
HHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ILSLPATTITPVIFIAVIFVLGFFMSLGKAAVYKHIPAYYPESVGAVGGIVGMMGGLGGF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ILPIAFGLLKDMTGLWSSCFLLLFAIVAISLVWMHLSVKQLSRQGPSEPVAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SVIEKAQPMSPGEPAKALWISTVAFTLCFAVWTIFAIIGIRIKQDLGLNEAEFGLLIGT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECC
PVLTGSLVRIVLGIWTGRYGGRLVYTLTMLAAALATFLLSYAQTYTQMLIAGLGVGLAGG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SFAVGVAYVSPFFPAEKQGTALGIFGAGNVGAAVTKFAAPFVLLAWGWQSVAEIWAVCLA
HHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LMAIVFWFTTSDDPAFRLRRERGTASKSLAQEFAPLQNVQVWRFSLYYFFAFGGFVALSL
HHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
WLPRYLVGVYGFNLETAGMIAAAYSIPGSIFRAFGGVLSDKKGARSVMYAMLAVSAVATL
HHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ILSLPATTITPVIFIAVIFVLGFFMSLGKAAVYKHIPAYYPESVGAVGGIVGMMGGLGGF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ILPIAFGLLKDMTGLWSSCFLLLFAIVAISLVWMHLSVKQLSRQGPSEPVAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]