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The map label for this gene is nasD [H]

Identifier: 209549055

GI number: 209549055

Start: 1475805

End: 1478255

Strand: Reverse

Name: nasD [H]

Synonym: Rleg2_1452

Alternate gene names: 209549055

Gene position: 1478255-1475805 (Counterclockwise)

Preceding gene: 209549056

Following gene: 209549054

Centisome position: 32.58

GC content: 62.22

Gene sequence:

>2451_bases
ATGACAGAAAAACTCGTCATCATCGGCAATGGCATGGCGCCCGGGCGCATGCTGGAGCACCTCTTCGAACGGGCGCCCGG
ACGCTATGAAGTCACGATCTTCAATGCCGAGCCGCGCGTCAATTACGACCGCATCATGCTGTCGCCGGTTCTCTCGGGAG
AAAAGGACTACGAGCAGATCATCATTCACGGTGACGGCTGGTACATCAAGCACGGCATCATGCTCTACAAGGGCCACAAG
ATCGTCAACATCGATCGCGATGCCAAGACGGTCACCTCCGATCATGGCGTCACCGAAAGCTACGACAAGCTGGTGATCGC
CACCGGTTCCGTGCCCTTCATCATCCCGGTTCCCGGCAAGGACCTGCCCGGCGTCATTACCTATCGCGATCTCGACGACG
TGCAGGCCATGCTGCTTGCCGCCCAGTCGCGCGAAAAGGCCGTCGTCATCGGCGGCGGTCTTTTGGGCCTCGAAGCGGCG
GCCGGCCTTGCCCAGCGCGGCATGGACGTCACCGTCCTGCACGTCATGCCGACGCTGATGGAGCGCCAGCTCGATCCCGC
CGCCGGTTATCTCTTGCAAAAGGCGGTCGAAGAACGCGGCATCAAGGTCATCTGCAAGGCCAATACCAAGGCGATCATCG
GCAACGGCAAGGTCGAAGGCATCGAACTCGACGACGGCCGCATCATCCCGGCAACCCTCGTCGTCATGGCCGTCGGCATT
CGTCCGAGTGTTGGCCTGGCGAAGGACGCCGGCCTTGCGGTCAATCGCGGCATCGTCGTCGATGCCGGCATGCAGACTTC
GGATGGCGACATCTTTGCGCTCGGCGAATGCGCCGAGGTCGGCGGCATGGTCTACGGCCTCGTCGCCCCGCTCTACGAGA
TGGCCCGCATTGCCGCGGCACATCTCTCGGACGACCGTTCGCCGGCTTTCGTGCATGCGGACACGCCGACCAAGCTGAAG
GTCACCGGCATCGAGCTCTATTCGCTCGGTGATTTCGCCGATGGCGACGACCGCGAGGAGATCGTGCTGCGTGACGCCAG
CGCCGGCGTCTACAAGCGTCTCGTGCTGAAGGACAACAAGATCATCGGCACCGTGCTTTACGGCGAGACCGCCGACGGCG
CCTGGTTCAACGATCTGAAGAAGAAGGCGACCGATATTTCGGAGATGCGCGAGACGCTGATCTTCGGTCAGGCCTATCAG
GGAGGGTCGCCGCTGGACCCTATGGCGGCCGTTGCAGCCTTGCCGGATGACGCGGAGATTTGTGGCTGCAACGGCGTATG
CAAGGGCAAGATTACCTCGACGATCACCAGCAAAGGCCTCACGTCGCTCGACGACGTGCGCGCCCATACCAAGGCATCCG
CCTCCTGCGGCTCCTGCACCGGCCTTGTCGAACAGCTGATGGCGCTGACCCTCGGCGATGGCTACAATCCGGCTGCCGTG
CAGCCGATGTGCACCTGCACCGAACTCGGCCATGACGACGTTCGCCGCCTGATCAAGGCCAAGGGTCTGAAGAGCATCCC
GGCCGTCATGCAGGAATTGGAATGGAAGACCTCTTGCGGCTGCGCCAAGTGTCGGCCGGCGCTCAATTATTACCTCGTGT
GCGACTGGCCGGACGAATATGCCGACGACTATCAGTCGCGCTTCATCAATGAGCGTGTCCACGCCAACATCCAGAAGGAC
GGCACCTATTCCGTCGTTCCCCGCATGTGGGGCGGCGTCACCAATTCGAACGAGTTGCGCGCCATCGCCGATGTCGTCGA
CAAGTTCGAGATCCCGATGGTGAAGGTGACGGGCGGCCAGCGCATCGACCTGCTCGGCATCGAGAAGGAAGATCTGCCCG
CCGTCTGGGCCGATCTCGGCAAGGCCGGTTTCGTCTCCGGCCAGGCCTATGCCAAGGGCCTGCGCACGGTGAAGACCTGC
GTCGGTTCGGACTGGTGCCGCTTCGGCACGCAGGATTCCACCGGTCTCGGCATCCGCATCGAGAAATTCATGTGGGGCTC
GTGGACGCCGGCCAAGCTGAAGATGGCCGTCTCCGGCTGCCCGCGCAATTGCGCCGAAGCAACCTGCAAGGATATCGGCG
TGATCTGCGTGGATTCCGGTTTCGAGATCCATTTCGCCGGTGCGGCCGGTCTCGACATCAAGGGCACCGAGGTGCTCGGC
CTGGTGAAGACCGAGGACGAGGCGCTGGAGCATATCGTGGCGCTGACGCAAATGTATCGCGAGCAAGCCCGCTATCTCGA
GCGCATCTACAAATGGGCCAAGCGCGTCGGACTGGAGGAAATCCGCCGCCAGATCATGGGCGATGCCGAAAAGCGCAAGG
CCTATTACGAGCGCTTCGTCTTCAGCCAGAAATTTGCCCAGGTCGATCCCTGGTCGGAGCGCGTCTCCGGCAAGGACAAG
CATGAATTCAAGCCGATGGCGACGATCGGCTATCCGCAGGCAGCCGAGTAA

Upstream 100 bases:

>100_bases
TCGCGATCTCGCTCGTCTGGATGCACCTGTCCGTCAAGCAATTGTCGCGCCAGGGACCCTCTGAGCCCGTGGCTGCAACC
TGATCTAAGGACTAGGAATT

Downstream 100 bases:

>100_bases
GGAGATGGATATGAATTGGCCCAATGAAAACTGGCATCCAATCGGCGACATTTCCGACATTCCCTTGCGTGGTGCCCGCT
GTGTGAAGACGCCTCAGGGC

Product: nitrite reductase (NAD(P)H), large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MTEKLVIIGNGMAPGRMLEHLFERAPGRYEVTIFNAEPRVNYDRIMLSPVLSGEKDYEQIIIHGDGWYIKHGIMLYKGHK
IVNIDRDAKTVTSDHGVTESYDKLVIATGSVPFIIPVPGKDLPGVITYRDLDDVQAMLLAAQSREKAVVIGGGLLGLEAA
AGLAQRGMDVTVLHVMPTLMERQLDPAAGYLLQKAVEERGIKVICKANTKAIIGNGKVEGIELDDGRIIPATLVVMAVGI
RPSVGLAKDAGLAVNRGIVVDAGMQTSDGDIFALGECAEVGGMVYGLVAPLYEMARIAAAHLSDDRSPAFVHADTPTKLK
VTGIELYSLGDFADGDDREEIVLRDASAGVYKRLVLKDNKIIGTVLYGETADGAWFNDLKKKATDISEMRETLIFGQAYQ
GGSPLDPMAAVAALPDDAEICGCNGVCKGKITSTITSKGLTSLDDVRAHTKASASCGSCTGLVEQLMALTLGDGYNPAAV
QPMCTCTELGHDDVRRLIKAKGLKSIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFINERVHANIQKD
GTYSVVPRMWGGVTNSNELRAIADVVDKFEIPMVKVTGGQRIDLLGIEKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTC
VGSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGFEIHFAGAAGLDIKGTEVLG
LVKTEDEALEHIVALTQMYREQARYLERIYKWAKRVGLEEIRRQIMGDAEKRKAYYERFVFSQKFAQVDPWSERVSGKDK
HEFKPMATIGYPQAAE

Sequences:

>Translated_816_residues
MTEKLVIIGNGMAPGRMLEHLFERAPGRYEVTIFNAEPRVNYDRIMLSPVLSGEKDYEQIIIHGDGWYIKHGIMLYKGHK
IVNIDRDAKTVTSDHGVTESYDKLVIATGSVPFIIPVPGKDLPGVITYRDLDDVQAMLLAAQSREKAVVIGGGLLGLEAA
AGLAQRGMDVTVLHVMPTLMERQLDPAAGYLLQKAVEERGIKVICKANTKAIIGNGKVEGIELDDGRIIPATLVVMAVGI
RPSVGLAKDAGLAVNRGIVVDAGMQTSDGDIFALGECAEVGGMVYGLVAPLYEMARIAAAHLSDDRSPAFVHADTPTKLK
VTGIELYSLGDFADGDDREEIVLRDASAGVYKRLVLKDNKIIGTVLYGETADGAWFNDLKKKATDISEMRETLIFGQAYQ
GGSPLDPMAAVAALPDDAEICGCNGVCKGKITSTITSKGLTSLDDVRAHTKASASCGSCTGLVEQLMALTLGDGYNPAAV
QPMCTCTELGHDDVRRLIKAKGLKSIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFINERVHANIQKD
GTYSVVPRMWGGVTNSNELRAIADVVDKFEIPMVKVTGGQRIDLLGIEKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTC
VGSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGFEIHFAGAAGLDIKGTEVLG
LVKTEDEALEHIVALTQMYREQARYLERIYKWAKRVGLEEIRRQIMGDAEKRKAYYERFVFSQKFAQVDPWSERVSGKDK
HEFKPMATIGYPQAAE
>Mature_815_residues
TEKLVIIGNGMAPGRMLEHLFERAPGRYEVTIFNAEPRVNYDRIMLSPVLSGEKDYEQIIIHGDGWYIKHGIMLYKGHKI
VNIDRDAKTVTSDHGVTESYDKLVIATGSVPFIIPVPGKDLPGVITYRDLDDVQAMLLAAQSREKAVVIGGGLLGLEAAA
GLAQRGMDVTVLHVMPTLMERQLDPAAGYLLQKAVEERGIKVICKANTKAIIGNGKVEGIELDDGRIIPATLVVMAVGIR
PSVGLAKDAGLAVNRGIVVDAGMQTSDGDIFALGECAEVGGMVYGLVAPLYEMARIAAAHLSDDRSPAFVHADTPTKLKV
TGIELYSLGDFADGDDREEIVLRDASAGVYKRLVLKDNKIIGTVLYGETADGAWFNDLKKKATDISEMRETLIFGQAYQG
GSPLDPMAAVAALPDDAEICGCNGVCKGKITSTITSKGLTSLDDVRAHTKASASCGSCTGLVEQLMALTLGDGYNPAAVQ
PMCTCTELGHDDVRRLIKAKGLKSIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFINERVHANIQKDG
TYSVVPRMWGGVTNSNELRAIADVVDKFEIPMVKVTGGQRIDLLGIEKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCV
GSDWCRFGTQDSTGLGIRIEKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGFEIHFAGAAGLDIKGTEVLGL
VKTEDEALEHIVALTQMYREQARYLERIYKWAKRVGLEEIRRQIMGDAEKRKAYYERFVFSQKFAQVDPWSERVSGKDKH
EFKPMATIGYPQAAE

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=238, Percent_Identity=28.9915966386555, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI21389617, Length=238, Percent_Identity=28.9915966386555, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI226437568, Length=238, Percent_Identity=28.9915966386555, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI4757732, Length=219, Percent_Identity=28.7671232876712, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI22202629, Length=219, Percent_Identity=28.7671232876712, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1789765, Length=808, Percent_Identity=35.519801980198, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789065, Length=342, Percent_Identity=28.6549707602339, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1788892, Length=281, Percent_Identity=29.5373665480427, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=406, Percent_Identity=24.6305418719212, Blast_Score=107, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281359715, Length=255, Percent_Identity=27.0588235294118, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281359713, Length=255, Percent_Identity=27.0588235294118, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24639250, Length=255, Percent_Identity=27.0588235294118, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI18543267, Length=255, Percent_Identity=27.0588235294118, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24639252, Length=255, Percent_Identity=27.0588235294118, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24639257, Length=255, Percent_Identity=27.0588235294118, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585130, Length=311, Percent_Identity=24.4372990353698, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24581020, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573993, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88817; Mature: 88686

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKLVIIGNGMAPGRMLEHLFERAPGRYEVTIFNAEPRVNYDRIMLSPVLSGEKDYEQI
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHHCCCCCCEEE
IIHGDGWYIKHGIMLYKGHKIVNIDRDAKTVTSDHGVTESYDKLVIATGSVPFIIPVPGK
EEECCCEEEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCC
DLPGVITYRDLDDVQAMLLAAQSREKAVVIGGGLLGLEAAAGLAQRGMDVTVLHVMPTLM
CCCCEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHHH
ERQLDPAAGYLLQKAVEERGIKVICKANTKAIIGNGKVEGIELDDGRIIPATLVVMAVGI
HHCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCEEEEEEECCCEEHHHHHHHHHHCC
RPSVGLAKDAGLAVNRGIVVDAGMQTSDGDIFALGECAEVGGMVYGLVAPLYEMARIAAA
CCCCCCCCCCCCEECCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
HLSDDRSPAFVHADTPTKLKVTGIELYSLGDFADGDDREEIVLRDASAGVYKRLVLKDNK
HCCCCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHEEECCC
IIGTVLYGETADGAWFNDLKKKATDISEMRETLIFGQAYQGGSPLDPMAAVAALPDDAEI
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCE
CGCNGVCKGKITSTITSKGLTSLDDVRAHTKASASCGSCTGLVEQLMALTLGDGYNPAAV
ECCCCEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QPMCTCTELGHDDVRRLIKAKGLKSIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEY
CCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHHH
ADDYQSRFINERVHANIQKDGTYSVVPRMWGGVTNSNELRAIADVVDKFEIPMVKVTGGQ
HHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
RIDLLGIEKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIRI
EEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEE
EKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGFEIHFAGAAGLDIKGTEVLG
EEEECCCCCHHHEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCEEEE
LVKTEDEALEHIVALTQMYREQARYLERIYKWAKRVGLEEIRRQIMGDAEKRKAYYERFV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHH
FSQKFAQVDPWSERVSGKDKHEFKPMATIGYPQAAE
HHHHHHCCCCHHHHCCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TEKLVIIGNGMAPGRMLEHLFERAPGRYEVTIFNAEPRVNYDRIMLSPVLSGEKDYEQI
CCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHHCCCCCCEEE
IIHGDGWYIKHGIMLYKGHKIVNIDRDAKTVTSDHGVTESYDKLVIATGSVPFIIPVPGK
EEECCCEEEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCC
DLPGVITYRDLDDVQAMLLAAQSREKAVVIGGGLLGLEAAAGLAQRGMDVTVLHVMPTLM
CCCCEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHHH
ERQLDPAAGYLLQKAVEERGIKVICKANTKAIIGNGKVEGIELDDGRIIPATLVVMAVGI
HHCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCEEEEEEECCCEEHHHHHHHHHHCC
RPSVGLAKDAGLAVNRGIVVDAGMQTSDGDIFALGECAEVGGMVYGLVAPLYEMARIAAA
CCCCCCCCCCCCEECCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
HLSDDRSPAFVHADTPTKLKVTGIELYSLGDFADGDDREEIVLRDASAGVYKRLVLKDNK
HCCCCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHEEECCC
IIGTVLYGETADGAWFNDLKKKATDISEMRETLIFGQAYQGGSPLDPMAAVAALPDDAEI
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCE
CGCNGVCKGKITSTITSKGLTSLDDVRAHTKASASCGSCTGLVEQLMALTLGDGYNPAAV
ECCCCEECCCHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QPMCTCTELGHDDVRRLIKAKGLKSIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEY
CCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHHH
ADDYQSRFINERVHANIQKDGTYSVVPRMWGGVTNSNELRAIADVVDKFEIPMVKVTGGQ
HHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
RIDLLGIEKEDLPAVWADLGKAGFVSGQAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIRI
EEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEE
EKFMWGSWTPAKLKMAVSGCPRNCAEATCKDIGVICVDSGFEIHFAGAAGLDIKGTEVLG
EEEECCCCCHHHEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCEEEE
LVKTEDEALEHIVALTQMYREQARYLERIYKWAKRVGLEEIRRQIMGDAEKRKAYYERFV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHH
FSQKFAQVDPWSERVSGKDKHEFKPMATIGYPQAAE
HHHHHHCCCCHHHHCCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]