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The map label for this gene is alaXM [H]

Identifier: 209549040

GI number: 209549040

Start: 1461168

End: 1461905

Strand: Reverse

Name: alaXM [H]

Synonym: Rleg2_1437

Alternate gene names: 209549040

Gene position: 1461905-1461168 (Counterclockwise)

Preceding gene: 209549041

Following gene: 209549039

Centisome position: 32.22

GC content: 61.65

Gene sequence:

>738_bases
ATGCCCGTCAATGCCCTCTATCGTGACGACTTTTATCTCTCGACATGCGAGGCGGTCGTCACCGCCGTCCACGAAGACGG
GGGCATCGAACTCGACCAGACCTGCTTCTATGCGACATCAGGCGGCCAGCCGGGTGATAGCGGTGAGCTTGAACGCGCCG
ACGGCACCAAGATCGTGTTGGGCCAGACGAAGCACGGCCCGAGCAAGGATATCGTCATCCATGTGCCGCTCGAAAACGAG
CCGCGGCCTCTGGTTGGCGAGACGCTGGTGCTGCATGTCGACTGGCAGCGCCGCTACAGGCTGATGCGCATGCACACGGC
CTGCCATCTGCTGTCCGTCGTCTGCTCCTATCCGATCACCGGCGCTGCCGTCGGCGAGGAGGAAAGCCGTGTCGACTTCG
ACATGGTCGAGACGATCGACAAGGACGAAGTGACAGCCAAGCTGATGGAACTGGTCGGCGAGAACCATCCGGTCTATCTG
CAATGGATTACCGACGAGGACCTTGCGGCCAATCCCGACATCGTCAAATCGAAGAACGTGCGCCCGCCGATCGGGCTCGG
GCGCGTCAGCCTCGTCTGCATCGGCGAGAACTCCTCGATTGACAGCCAGCCCTGCGGCGGCACACATGTCTCCGAGACAC
AGGAAGTCGGGCAGATTCACATCGCCAAAATCGAAAAGAAGGGCAAGGAGAACCGGCGCTTCCGCATCCGTTTCGGCACG
CCCGGCGACGAAGCCTGA

Upstream 100 bases:

>100_bases
CTTCAATCCGGATTTCCTGACCTCGAAGGGACTGCCCGTTCCGGGCTGGATGGCCAAGTCGCCCGAGATAGACGTTCCCT
ACGAACCCGCGTGAGACCCC

Downstream 100 bases:

>100_bases
CCATCAAGGGAGAGCATCATGAGTGAGACCAAGAGCCGTTTCGTCGTCTCGGCCGACTGGCTGCAGGCCGAGCTCGGCAA
GCCGGATCTGCGCGTACTGG

Product: threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPVNALYRDDFYLSTCEAVVTAVHEDGGIELDQTCFYATSGGQPGDSGELERADGTKIVLGQTKHGPSKDIVIHVPLENE
PRPLVGETLVLHVDWQRRYRLMRMHTACHLLSVVCSYPITGAAVGEEESRVDFDMVETIDKDEVTAKLMELVGENHPVYL
QWITDEDLAANPDIVKSKNVRPPIGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGQIHIAKIEKKGKENRRFRIRFGT
PGDEA

Sequences:

>Translated_245_residues
MPVNALYRDDFYLSTCEAVVTAVHEDGGIELDQTCFYATSGGQPGDSGELERADGTKIVLGQTKHGPSKDIVIHVPLENE
PRPLVGETLVLHVDWQRRYRLMRMHTACHLLSVVCSYPITGAAVGEEESRVDFDMVETIDKDEVTAKLMELVGENHPVYL
QWITDEDLAANPDIVKSKNVRPPIGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGQIHIAKIEKKGKENRRFRIRFGT
PGDEA
>Mature_244_residues
PVNALYRDDFYLSTCEAVVTAVHEDGGIELDQTCFYATSGGQPGDSGELERADGTKIVLGQTKHGPSKDIVIHVPLENEP
RPLVGETLVLHVDWQRRYRLMRMHTACHLLSVVCSYPITGAAVGEEESRVDFDMVETIDKDEVTAKLMELVGENHPVYLQ
WITDEDLAANPDIVKSKNVRPPIGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGQIHIAKIEKKGKENRRFRIRFGTP
GDEA

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) or Gly-tRNA(Ala). Has no activity on incorrectly charged Ser-tRNA(Thr), nor on correctly charged Ala-tRNA(Ala) or Ser-tRNA(Ser) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=232, Percent_Identity=30.1724137931034, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324911, Length=241, Percent_Identity=27.3858921161826, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 27036; Mature: 26905

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVNALYRDDFYLSTCEAVVTAVHEDGGIELDQTCFYATSGGQPGDSGELERADGTKIVL
CCCCCEECCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCCCEECCCCCEEEE
GQTKHGPSKDIVIHVPLENEPRPLVGETLVLHVDWQRRYRLMRMHTACHLLSVVCSYPIT
ECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GAAVGEEESRVDFDMVETIDKDEVTAKLMELVGENHPVYLQWITDEDLAANPDIVKSKNV
CCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCC
RPPIGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGQIHIAKIEKKGKENRRFRIRFGT
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHCEEEEEEEHHCCCCCCEEEEEECC
PGDEA
CCCCC
>Mature Secondary Structure 
PVNALYRDDFYLSTCEAVVTAVHEDGGIELDQTCFYATSGGQPGDSGELERADGTKIVL
CCCCEECCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCCCEECCCCCEEEE
GQTKHGPSKDIVIHVPLENEPRPLVGETLVLHVDWQRRYRLMRMHTACHLLSVVCSYPIT
ECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GAAVGEEESRVDFDMVETIDKDEVTAKLMELVGENHPVYLQWITDEDLAANPDIVKSKNV
CCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCC
RPPIGLGRVSLVCIGENSSIDSQPCGGTHVSETQEVGQIHIAKIEKKGKENRRFRIRFGT
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHCEEEEEEEHHCCCCCCEEEEEECC
PGDEA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA