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The map label for this gene is sseA [H]

Identifier: 209549039

GI number: 209549039

Start: 1460280

End: 1461149

Strand: Reverse

Name: sseA [H]

Synonym: Rleg2_1436

Alternate gene names: 209549039

Gene position: 1461149-1460280 (Counterclockwise)

Preceding gene: 209549040

Following gene: 209549038

Centisome position: 32.2

GC content: 65.52

Gene sequence:

>870_bases
ATGAGTGAGACCAAGAGCCGTTTCGTCGTCTCGGCCGACTGGCTGCAGGCCGAGCTCGGCAAGCCGGATCTGCGCGTACT
GGACGCCTCCTTCTATCTGCCGGCGCAGAAACGCGATGCCGATGCCGAATATGCGGCCGGCCATATTCCCGGCGCCATCC
GCTTCGACCAAGACAAGATCGCCGACCATTCGACGTCACTGCCGCACACGATCCCCTCGTCCGATTATTTCGCCACTGAG
GCCGGCCGGCTCGGCATCAGCGAGAACGATCGCATCGTCGTCTATGACGGCATCGGCCTGTTCGCCTCGCCGCGCGTCTG
GTGGCTGTTCCGGATGATGGGCGCAAAGAACGTCTTCGTGCTCGATGGCGGTCTCGACGGCTGGAAGGCCGAAGGCCGCC
CGCTCGAAACCACCGTGCCCAGCTATTCGCCGGCGCGTTTCACGCCCGATTTCGACGAGAGCCGCGTGGTGACGCTCGAT
ACGATGCGCGATATCGTCTCCAGCGGCGCGATGCAGATCGCCGATGCCCGCAGCGCCGGCCGCTTCGCCGCCGCCGAGCC
CGAGCCGCGTGCCGGCATGCGCTCCGGCCATATGCCCGGCGCCCGCAACTTGCCCTCTGGCGTTTTCGCCAACCAGGGCC
GGTTCAAGTCACTGCCCGAACTGCGCCAGACGATCGAGGATGCCGGCATCGATCTTTCCAAGCCCGTTGTGACCTCCTGC
GGTTCGGGCATCACCGCCGCGATCATCACGCTGGCGCTGGAATCGCTCGGCCATCACGACAACAAGCTCTACGACGGCTC
GTGGAGCGAATGGGGCAGCCGTGACGATACGCCGATCGTCACCGGTCCGCCGACGCCGGTTAAAGCCTGA

Upstream 100 bases:

>100_bases
TCACATCGCCAAAATCGAAAAGAAGGGCAAGGAGAACCGGCGCTTCCGCATCCGTTTCGGCACGCCCGGCGACGAAGCCT
GACCATCAAGGGAGAGCATC

Downstream 100 bases:

>100_bases
TCGCCATGGGGAAGACACCCGCTTCTTTGAAAGCTCACATCACCCGGCTCGAAATGACGGCGGCGCCGAAGGCGAGCATG
CCGGTGCCCGTCAATATCCA

Product: rhodanese

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSETKSRFVVSADWLQAELGKPDLRVLDASFYLPAQKRDADAEYAAGHIPGAIRFDQDKIADHSTSLPHTIPSSDYFATE
AGRLGISENDRIVVYDGIGLFASPRVWWLFRMMGAKNVFVLDGGLDGWKAEGRPLETTVPSYSPARFTPDFDESRVVTLD
TMRDIVSSGAMQIADARSAGRFAAAEPEPRAGMRSGHMPGARNLPSGVFANQGRFKSLPELRQTIEDAGIDLSKPVVTSC
GSGITAAIITLALESLGHHDNKLYDGSWSEWGSRDDTPIVTGPPTPVKA

Sequences:

>Translated_289_residues
MSETKSRFVVSADWLQAELGKPDLRVLDASFYLPAQKRDADAEYAAGHIPGAIRFDQDKIADHSTSLPHTIPSSDYFATE
AGRLGISENDRIVVYDGIGLFASPRVWWLFRMMGAKNVFVLDGGLDGWKAEGRPLETTVPSYSPARFTPDFDESRVVTLD
TMRDIVSSGAMQIADARSAGRFAAAEPEPRAGMRSGHMPGARNLPSGVFANQGRFKSLPELRQTIEDAGIDLSKPVVTSC
GSGITAAIITLALESLGHHDNKLYDGSWSEWGSRDDTPIVTGPPTPVKA
>Mature_288_residues
SETKSRFVVSADWLQAELGKPDLRVLDASFYLPAQKRDADAEYAAGHIPGAIRFDQDKIADHSTSLPHTIPSSDYFATEA
GRLGISENDRIVVYDGIGLFASPRVWWLFRMMGAKNVFVLDGGLDGWKAEGRPLETTVPSYSPARFTPDFDESRVVTLDT
MRDIVSSGAMQIADARSAGRFAAAEPEPRAGMRSGHMPGARNLPSGVFANQGRFKSLPELRQTIEDAGIDLSKPVVTSCG
SGITAAIITLALESLGHHDNKLYDGSWSEWGSRDDTPIVTGPPTPVKA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI61835204, Length=276, Percent_Identity=40.9420289855072, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI194473681, Length=276, Percent_Identity=40.9420289855072, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI194473668, Length=276, Percent_Identity=40.9420289855072, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI17402865, Length=276, Percent_Identity=42.0289855072464, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI87082121, Length=275, Percent_Identity=39.2727272727273, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI87081967, Length=279, Percent_Identity=25.089605734767, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71997283, Length=301, Percent_Identity=27.2425249169435, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17561888, Length=304, Percent_Identity=25.9868421052632, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17559150, Length=294, Percent_Identity=27.5510204081633, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI115534702, Length=266, Percent_Identity=25.187969924812, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543836, Length=288, Percent_Identity=26.7361111111111, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543838, Length=288, Percent_Identity=26.7361111111111, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324825, Length=266, Percent_Identity=35.7142857142857, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31309; Mature: 31178

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETKSRFVVSADWLQAELGKPDLRVLDASFYLPAQKRDADAEYAAGHIPGAIRFDQDKI
CCCCCCEEEEEHHHHHHHCCCCCEEEEECEEECCCCCCCCCCHHHCCCCCCEEEECHHHH
ADHSTSLPHTIPSSDYFATEAGRLGISENDRIVVYDGIGLFASPRVWWLFRMMGAKNVFV
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEE
LDGGLDGWKAEGRPLETTVPSYSPARFTPDFDESRVVTLDTMRDIVSSGAMQIADARSAG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEHHCCCCC
RFAAAEPEPRAGMRSGHMPGARNLPSGVFANQGRFKSLPELRQTIEDAGIDLSKPVVTSC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHC
GSGITAAIITLALESLGHHDNKLYDGSWSEWGSRDDTPIVTGPPTPVKA
CCCHHHHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
SETKSRFVVSADWLQAELGKPDLRVLDASFYLPAQKRDADAEYAAGHIPGAIRFDQDKI
CCCCCEEEEEHHHHHHHCCCCCEEEEECEEECCCCCCCCCCHHHCCCCCCEEEECHHHH
ADHSTSLPHTIPSSDYFATEAGRLGISENDRIVVYDGIGLFASPRVWWLFRMMGAKNVFV
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEE
LDGGLDGWKAEGRPLETTVPSYSPARFTPDFDESRVVTLDTMRDIVSSGAMQIADARSAG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEHHCCCCC
RFAAAEPEPRAGMRSGHMPGARNLPSGVFANQGRFKSLPELRQTIEDAGIDLSKPVVTSC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHC
GSGITAAIITLALESLGHHDNKLYDGSWSEWGSRDDTPIVTGPPTPVKA
CCCHHHHHHHHHHHHHCCCCCEEECCCCHHCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]