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The map label for this gene is yneF [H]

Identifier: 209548849

GI number: 209548849

Start: 1260384

End: 1261706

Strand: Reverse

Name: yneF [H]

Synonym: Rleg2_1246

Alternate gene names: 209548849

Gene position: 1261706-1260384 (Counterclockwise)

Preceding gene: 209548856

Following gene: 209548848

Centisome position: 27.8

GC content: 65.99

Gene sequence:

>1323_bases
ATGCAGGCCAACAAATCGGGGCCGGCAGACAAGGCGACCGGTAAGACAGACGGGCAAGGGGTTGCTGCCGTGTTCAATAT
CGCTACAGGTCTCGCCATCTGGTGTTCGGAGGCCATGACGCTCGCTTTGGTGCTGTTCGTCGCCTGGCGCCACAATGTCC
GGAACGAGGCCTATCTCTATTGGGGCCTCGGCTTTCTGCTGACCGGCATCGGCTTTGCGATGATCGCGCTGCGCGACCAG
GTCCCCAGCCTGCTGTCGATAGAGATCGGCAATGCCATCGCGCTGCTCGGCCAAAGCGCCTGGGTGGCGGGCTTTCTGGC
GCTCGACCGTAAGAAGATCGAGTGGTGGGCGCTGCTGCCGCCGGCAATCTGGCTCGCCGGGGTCTTCCTGCCCTGGGTCA
ACAGCGACTATTCAAACCGGGTGGTGCTCTACAATCTCGCTTCGGCGACGGGTGCGACGGCGCTTGCCCTGGCGGTCGCC
GCCGGCGACATGCGCCGCGAGCGTACCCGCGTCAAGCTGATGGGCGTCTTCGTCATCCAGGGCTGCCTGTGCTTCGGCTC
GGCGCTGACGATGGCGCTGACGCTGCCAAGCGATATCGAGGCAACCAATCTCGGCGGCGCCTCCGCCATGGCAAGCGCCT
TCCTGCTCACCATCGCCTTCGCCTTCACCTGCCGGCTGATCATGGAGCGCTCCGAGCGGCACCTGCGCGCCCTCACCCTC
ACCGATTCGCTGACCGGTGTGCTCAACCGCCGCGGCCTGCTCGGCTATTTCGACGCCATCCAGGAAAAAGCCCATGACGA
GCAACACCAGGTCGCGGTGATGCTGTTCGATCTCGACCATTTCAAGCGCGTCAACGACCGTTTCGGCCATCAGTCCGGCG
ATGCCGTGCTGACCGCTTTCGCCCGCATGGCCCGCCAGTATATTCCGAACAGCATCTTCGGCCGCATGGGCGGCGAAGAA
TTCGCAGCCTTCGCCGCGGTCACGGACCAGACCGAAGCCGAGGCGATCGCCGAAGCGATCCGCACCGAATTCTGCCGCCT
GCCCGTCAGCACCGGCGAAGCCATCGTCCCGGTCACCGTCAGCATCGGCATCGCGCTCGCCTCCTCGCTCGAAGCCAATA
TCGACAAGCTGATCTCGGCCGCCGACCGGGCGCTCTATGCCGCGAAGGCCGCCGGCCGCAACTGCACGGTCACCTTCGGC
GAAGCCGAGGCGGCAGCCCCTGCACCGGCGACGCCCAGCACCACCGCCGGCGAACTCGTGCCGACGGTCGACGACCAGGT
CGAGGCGCTGCGGCGCATGGGTACGCTGTCGCGGGCCGGTTAA

Upstream 100 bases:

>100_bases
CACACACATTTACCCTAGGGAAAGCCTACCGGCAGATATCGGCGCAAAACCATGCCGCAGAGCAGCAGCATTAAGGAAGT
GGCGAACTTCCTCGGCAAGG

Downstream 100 bases:

>100_bases
AGCTCTTTAGTTTTTGCGCGTGGTTGCCCCGGAACCGCTGCACAGCTCGGGGCAATGCGCATTTGGCAGCATCCTGAAAT
CAGAATCGAATCTTTGGGTT

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MQANKSGPADKATGKTDGQGVAAVFNIATGLAIWCSEAMTLALVLFVAWRHNVRNEAYLYWGLGFLLTGIGFAMIALRDQ
VPSLLSIEIGNAIALLGQSAWVAGFLALDRKKIEWWALLPPAIWLAGVFLPWVNSDYSNRVVLYNLASATGATALALAVA
AGDMRRERTRVKLMGVFVIQGCLCFGSALTMALTLPSDIEATNLGGASAMASAFLLTIAFAFTCRLIMERSERHLRALTL
TDSLTGVLNRRGLLGYFDAIQEKAHDEQHQVAVMLFDLDHFKRVNDRFGHQSGDAVLTAFARMARQYIPNSIFGRMGGEE
FAAFAAVTDQTEAEAIAEAIRTEFCRLPVSTGEAIVPVTVSIGIALASSLEANIDKLISAADRALYAAKAAGRNCTVTFG
EAEAAAPAPATPSTTAGELVPTVDDQVEALRRMGTLSRAG

Sequences:

>Translated_440_residues
MQANKSGPADKATGKTDGQGVAAVFNIATGLAIWCSEAMTLALVLFVAWRHNVRNEAYLYWGLGFLLTGIGFAMIALRDQ
VPSLLSIEIGNAIALLGQSAWVAGFLALDRKKIEWWALLPPAIWLAGVFLPWVNSDYSNRVVLYNLASATGATALALAVA
AGDMRRERTRVKLMGVFVIQGCLCFGSALTMALTLPSDIEATNLGGASAMASAFLLTIAFAFTCRLIMERSERHLRALTL
TDSLTGVLNRRGLLGYFDAIQEKAHDEQHQVAVMLFDLDHFKRVNDRFGHQSGDAVLTAFARMARQYIPNSIFGRMGGEE
FAAFAAVTDQTEAEAIAEAIRTEFCRLPVSTGEAIVPVTVSIGIALASSLEANIDKLISAADRALYAAKAAGRNCTVTFG
EAEAAAPAPATPSTTAGELVPTVDDQVEALRRMGTLSRAG
>Mature_440_residues
MQANKSGPADKATGKTDGQGVAAVFNIATGLAIWCSEAMTLALVLFVAWRHNVRNEAYLYWGLGFLLTGIGFAMIALRDQ
VPSLLSIEIGNAIALLGQSAWVAGFLALDRKKIEWWALLPPAIWLAGVFLPWVNSDYSNRVVLYNLASATGATALALAVA
AGDMRRERTRVKLMGVFVIQGCLCFGSALTMALTLPSDIEATNLGGASAMASAFLLTIAFAFTCRLIMERSERHLRALTL
TDSLTGVLNRRGLLGYFDAIQEKAHDEQHQVAVMLFDLDHFKRVNDRFGHQSGDAVLTAFARMARQYIPNSIFGRMGGEE
FAAFAAVTDQTEAEAIAEAIRTEFCRLPVSTGEAIVPVTVSIGIALASSLEANIDKLISAADRALYAAKAAGRNCTVTFG
EAEAAAPAPATPSTTAGELVPTVDDQVEALRRMGTLSRAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=167, Percent_Identity=35.9281437125749, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=33.5135135135135, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=36.6279069767442, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=37.888198757764, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=32.7586206896552, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=35.8823529411765, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=31.7073170731707, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693134, Length=202, Percent_Identity=33.1683168316832, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=31.2101910828025, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=36.0248447204969, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081921, Length=170, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=28.7292817679558, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=155, Percent_Identity=31.6129032258064, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 47008; Mature: 47008

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQANKSGPADKATGKTDGQGVAAVFNIATGLAIWCSEAMTLALVLFVAWRHNVRNEAYLY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
WGLGFLLTGIGFAMIALRDQVPSLLSIEIGNAIALLGQSAWVAGFLALDRKKIEWWALLP
ECHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCHHHHHHHHHHCCCCCEEEEECC
PAIWLAGVFLPWVNSDYSNRVVLYNLASATGATALALAVAAGDMRRERTRVKLMGVFVIQ
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCLCFGSALTMALTLPSDIEATNLGGASAMASAFLLTIAFAFTCRLIMERSERHLRALTL
HHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
TDSLTGVLNRRGLLGYFDAIQEKAHDEQHQVAVMLFDLDHFKRVNDRFGHQSGDAVLTAF
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEEEEEEEHHHHHHHHHHCCCCCCHHHHHHH
ARMARQYIPNSIFGRMGGEEFAAFAAVTDQTEAEAIAEAIRTEFCRLPVSTGEAIVPVTV
HHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEH
SIGIALASSLEANIDKLISAADRALYAAKAAGRNCTVTFGEAEAAAPAPATPSTTAGELV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
PTVDDQVEALRRMGTLSRAG
CCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQANKSGPADKATGKTDGQGVAAVFNIATGLAIWCSEAMTLALVLFVAWRHNVRNEAYLY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
WGLGFLLTGIGFAMIALRDQVPSLLSIEIGNAIALLGQSAWVAGFLALDRKKIEWWALLP
ECHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCHHHHHHHHHHCCCCCEEEEECC
PAIWLAGVFLPWVNSDYSNRVVLYNLASATGATALALAVAAGDMRRERTRVKLMGVFVIQ
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCLCFGSALTMALTLPSDIEATNLGGASAMASAFLLTIAFAFTCRLIMERSERHLRALTL
HHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
TDSLTGVLNRRGLLGYFDAIQEKAHDEQHQVAVMLFDLDHFKRVNDRFGHQSGDAVLTAF
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHEEEEEEEEHHHHHHHHHHCCCCCCHHHHHHH
ARMARQYIPNSIFGRMGGEEFAAFAAVTDQTEAEAIAEAIRTEFCRLPVSTGEAIVPVTV
HHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEH
SIGIALASSLEANIDKLISAADRALYAAKAAGRNCTVTFGEAEAAAPAPATPSTTAGELV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
PTVDDQVEALRRMGTLSRAG
CCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]