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The map label for this gene is pksS [H]

Identifier: 209548848

GI number: 209548848

Start: 1258931

End: 1260175

Strand: Reverse

Name: pksS [H]

Synonym: Rleg2_1245

Alternate gene names: 209548848

Gene position: 1260175-1258931 (Counterclockwise)

Preceding gene: 209548849

Following gene: 209548843

Centisome position: 27.77

GC content: 63.94

Gene sequence:

>1245_bases
ATGATGATACCGCCCTTTCTCTCGATCGACCGCTCAAGCCGCCACGTCTCGCTGGACGCCCGCAATCCGGCCTTCTACGG
CGATCCGAATGCCGTCTATGCCGCACTTCACGCCCATTGCCCGACCTTCTACTGGAGCGAACAGAAGCAGTGGTTCTTCA
CCGGCTACGACCATGTGAACGGACTGCTGCGCGACCGCCGATTCGGCCGGCAGATCCTGCACATCGCCAGCCGCGAGGAG
CTCGGCCTTGCCGAGCCGATGCCGCATCTTGCCAGTTTCGATCTCTCCGAACGCTATTCCCTGCTCGAACTCGAACCGCC
GGAGCACACGCGGCTGCGCACGCTGGTCAACCGCGCCTTCGTCTCCCGCCATGTCGAGAAGATGAAGCCGGAGCTTGCCG
AACTCGCCAACCAGCTGATCGACGGCTTTGCCGACAAGGGCGAGGTCGAGCTACTCTCGGCCTTTGCCGACATCATCCCG
GTGACGATGATCGCGCGGATGATCGGCATCCCCGAGGAGATGGGGCCGCAGCTGCTTGCCTGGTCGCACGCCTATGTCCG
CATGTACATGTTCGGCCGCACGCGCGAGCAGGAGGATGAGGCCGAACGGGCGGCGAAGGAATTTTCCGACTATGTCAGGA
CGGTGATTGCCGAGCGCCGCGCCGAGCCGCGCGACGACCTGCTCACCCACATGATCCATACCGAGCATAAGGGTCAGTAT
CTCACCGAGGAAGAGCTGATCTCGACGACGATCGTGCTGCTCAACGCCGGGCACGAAGCGACCGTGCACCAGATCGGCAA
TTCCGTGCGCACCATCCTCGAAAGCGGCTCGGATCCGGCGGACCTCTTCCGCGACGAGGCGACGACCGAGCGCACCGTCG
AGGAAACCCTGCGCATCTGCGCGCCGGTCCACATCTTCCAACGATGGGCGCTCGAGCCCGCCGAAATCGACGGCGTCTCC
TTCCAGCGCGGCGACAAGGTCAGTCTCATCCTCGCCGCCGCCAATCTCGATCCGGCGAAATTCACCGATCCACTCGCCTT
CCGGCCCGACCGCAACGAGGCGGCGAACCTTTCGTTCGGCGCCGGCATCCATTTCTGCATCGGCGCACCGCTGGCGCGAC
TGGAGCTCAACGTCGTGCTGCCGATCCTGTTCGAGCGGCTTGCCGGCCTGAAGCTGGCGAAGACGCCCGTGGTCAAGGAT
GTCTATCATTTCCACGGGCTGGACCGGCTGGATCTGCAGTGGTGA

Upstream 100 bases:

>100_bases
ACCTTAATGTGATGGTGCGTCCGGCCTAAAATCAACTGATCCAGGATCGCCCCTGCCCCGCATCTTTCCGAGGCCCGGCA
AATCCGCTAAGCCTCGGGGC

Downstream 100 bases:

>100_bases
TCCAGCCCTGACAGATCAGCCGCCGATCGCGCGTCAGGCGGCCGACTCTTCTTCCAGCTTGCTGGATATGTCGCGTGCCG
ACATCGGTCTGCCGATTAGA

Product: cytochrome P450

Products: ROH; oxidized flavoprotein; H2O

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MMIPPFLSIDRSSRHVSLDARNPAFYGDPNAVYAALHAHCPTFYWSEQKQWFFTGYDHVNGLLRDRRFGRQILHIASREE
LGLAEPMPHLASFDLSERYSLLELEPPEHTRLRTLVNRAFVSRHVEKMKPELAELANQLIDGFADKGEVELLSAFADIIP
VTMIARMIGIPEEMGPQLLAWSHAYVRMYMFGRTREQEDEAERAAKEFSDYVRTVIAERRAEPRDDLLTHMIHTEHKGQY
LTEEELISTTIVLLNAGHEATVHQIGNSVRTILESGSDPADLFRDEATTERTVEETLRICAPVHIFQRWALEPAEIDGVS
FQRGDKVSLILAAANLDPAKFTDPLAFRPDRNEAANLSFGAGIHFCIGAPLARLELNVVLPILFERLAGLKLAKTPVVKD
VYHFHGLDRLDLQW

Sequences:

>Translated_414_residues
MMIPPFLSIDRSSRHVSLDARNPAFYGDPNAVYAALHAHCPTFYWSEQKQWFFTGYDHVNGLLRDRRFGRQILHIASREE
LGLAEPMPHLASFDLSERYSLLELEPPEHTRLRTLVNRAFVSRHVEKMKPELAELANQLIDGFADKGEVELLSAFADIIP
VTMIARMIGIPEEMGPQLLAWSHAYVRMYMFGRTREQEDEAERAAKEFSDYVRTVIAERRAEPRDDLLTHMIHTEHKGQY
LTEEELISTTIVLLNAGHEATVHQIGNSVRTILESGSDPADLFRDEATTERTVEETLRICAPVHIFQRWALEPAEIDGVS
FQRGDKVSLILAAANLDPAKFTDPLAFRPDRNEAANLSFGAGIHFCIGAPLARLELNVVLPILFERLAGLKLAKTPVVKD
VYHFHGLDRLDLQW
>Mature_414_residues
MMIPPFLSIDRSSRHVSLDARNPAFYGDPNAVYAALHAHCPTFYWSEQKQWFFTGYDHVNGLLRDRRFGRQILHIASREE
LGLAEPMPHLASFDLSERYSLLELEPPEHTRLRTLVNRAFVSRHVEKMKPELAELANQLIDGFADKGEVELLSAFADIIP
VTMIARMIGIPEEMGPQLLAWSHAYVRMYMFGRTREQEDEAERAAKEFSDYVRTVIAERRAEPRDDLLTHMIHTEHKGQY
LTEEELISTTIVLLNAGHEATVHQIGNSVRTILESGSDPADLFRDEATTERTVEETLRICAPVHIFQRWALEPAEIDGVS
FQRGDKVSLILAAANLDPAKFTDPLAFRPDRNEAANLSFGAGIHFCIGAPLARLELNVVLPILFERLAGLKLAKTPVVKD
VYHFHGLDRLDLQW

Specific function: Involved in the metabolism of the antibiotic polyketide bacillaene which is involved in secondary metabolism. The substrate is dihydrobacillaene [H]

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: 1.14.14.1

Molecular weight: Translated: 47025; Mature: 47025

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIPPFLSIDRSSRHVSLDARNPAFYGDPNAVYAALHAHCPTFYWSEQKQWFFTGYDHVN
CCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEECHHHHH
GLLRDRRFGRQILHIASREELGLAEPMPHLASFDLSERYSLLELEPPEHTRLRTLVNRAF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHCCCEEECCCCCHHHHHHHHHHHH
VSRHVEKMKPELAELANQLIDGFADKGEVELLSAFADIIPVTMIARMIGIPEEMGPQLLA
HHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHH
WSHAYVRMYMFGRTREQEDEAERAAKEFSDYVRTVIAERRAEPRDDLLTHMIHTEHKGQY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
LTEEELISTTIVLLNAGHEATVHQIGNSVRTILESGSDPADLFRDEATTERTVEETLRIC
CCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
APVHIFQRWALEPAEIDGVSFQRGDKVSLILAAANLDPAKFTDPLAFRPDRNEAANLSFG
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCC
AGIHFCIGAPLARLELNVVLPILFERLAGLKLAKTPVVKDVYHFHGLDRLDLQW
CCCHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMIPPFLSIDRSSRHVSLDARNPAFYGDPNAVYAALHAHCPTFYWSEQKQWFFTGYDHVN
CCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEECHHHHH
GLLRDRRFGRQILHIASREELGLAEPMPHLASFDLSERYSLLELEPPEHTRLRTLVNRAF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHCCCEEECCCCCHHHHHHHHHHHH
VSRHVEKMKPELAELANQLIDGFADKGEVELLSAFADIIPVTMIARMIGIPEEMGPQLLA
HHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHH
WSHAYVRMYMFGRTREQEDEAERAAKEFSDYVRTVIAERRAEPRDDLLTHMIHTEHKGQY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
LTEEELISTTIVLLNAGHEATVHQIGNSVRTILESGSDPADLFRDEATTERTVEETLRIC
CCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
APVHIFQRWALEPAEIDGVSFQRGDKVSLILAAANLDPAKFTDPLAFRPDRNEAANLSFG
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCC
AGIHFCIGAPLARLELNVVLPILFERLAGLKLAKTPVVKDVYHFHGLDRLDLQW
CCCHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RH; reduced flavoprotein; O2

Specific reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]