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The map label for this gene is thcD [H]

Identifier: 209548588

GI number: 209548588

Start: 1006395

End: 1007612

Strand: Direct

Name: thcD [H]

Synonym: Rleg2_0985

Alternate gene names: 209548588

Gene position: 1006395-1007612 (Clockwise)

Preceding gene: 209548587

Following gene: 209548589

Centisome position: 22.18

GC content: 64.61

Gene sequence:

>1218_bases
GTGACGGATAGACTGGTGATCATCGGCGCGGGGCAGGCCGGTTTCGCAATGGCGGCCAAGCTGCGTGCCTTGAAGGACAC
GCGCCCGATCACCCTTATCGGCGCCGAAGACGTCGCGCCCTATCAGCGCCCGCCGCTTTCGAAGAAATACCTGCTCGGCG
AAATGGCCTTCGACCGTTTGCTGTTCCGCGACGAGCACTGGTATGGCGACAACGACGTCGACCTTCGCCTTTCCACATGG
GCCGAGCAGATCAAGCCGGACAGCAAGCAGGTTCTGCTGCAGGACGGCTCCGTTCTCGACTACGGCACGCTGGCGCTCGC
CACCGGCTCGACGCCGCGCCGGCTGCCGGCCGCAATCGGCGGCGATCTTGAAGGTGTCTATGTCGCCCGCGACAAGCGTG
ATGCCGATCTGCTCGCCGAGGAGATGCGCCCTGGTCGCCGCGTCCTCATCATCGGCGGCGGTTATATCGGCCTCGAGGCG
GCAGCCGTCGCCCGCCATCGCGGCCTTGAGGTCACCGTCATCGAGATGGCCGACCGCATCCTGCAGCGTGTCGCGGCGAA
GGAGACCGCCGACATCATGCGCGGGATCCATGAGTCCCACGACGTGGTGATCCGCGAGAAGACCGGGCTCAAGCATCTGG
TCGGCAAGGATGGCCGCGTCACCGGCGCCGCACTGTCCGACGGATCGGTTATCGATATCGACTTTGCCGTCGTCGGCATC
GGCGTCGTGCCGAACGACCTGCTCGCCAAGGAAGCCGGCCTCGAAGTCGCCAACGGCATCGTCGTCGACGAATTCGCCCG
CACCTCCGATCCGGCGATCTTTGCCGCCGGCGATTGCGCAGCCCTTCCCTGGCAGGGTGGCCGCATCCGGCTCGAATCGG
TGCAGAACGCCGTCGACCAGGCCGAAGCAGCGGCAGCTGTCATCGCCGGTGGCAGCGAGCCATATGACCCGAAGCCGTGG
TTCTGGTCCGACCAGTACGATGTCAAGCTGCAGATCGCCGGCTTCAATCTCGGTTATGACGAAACACTGCTGCGCCCAGG
CGCCCGCGAAGGCGCCCATTCCATCTGGTACTTCAAAGAAGGTCTGCTGATCGCCGTCGATGCCATCAACGACGCCAAAG
CCTATGTGACCGGTAAAAAGCTGTTGGAATCCGGGACCAACCCCGACCGATCGATTCTCGCCGACCCCTCTGGCGATCTC
AAGCGCCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
TCGAGATCGGCTGAAGGTAAGAAGCCGCTTCCGTTGACGGAGCATAGGGCAGGGGATAGGCGTTGGCATGAGTGTCGGGC
CTCGTTTCAGGAGTGAGATC

Downstream 100 bases:

>100_bases
GCTCAAAACCAATCGTCGCAAGGAATAACCTCCGGCGGAGCGCCGTGTGAAGCATGCCAATCCTAAAGGACGCCAAGCGG
CAAGCTGGCGTCTTCGTCGG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTDRLVIIGAGQAGFAMAAKLRALKDTRPITLIGAEDVAPYQRPPLSKKYLLGEMAFDRLLFRDEHWYGDNDVDLRLSTW
AEQIKPDSKQVLLQDGSVLDYGTLALATGSTPRRLPAAIGGDLEGVYVARDKRDADLLAEEMRPGRRVLIIGGGYIGLEA
AAVARHRGLEVTVIEMADRILQRVAAKETADIMRGIHESHDVVIREKTGLKHLVGKDGRVTGAALSDGSVIDIDFAVVGI
GVVPNDLLAKEAGLEVANGIVVDEFARTSDPAIFAAGDCAALPWQGGRIRLESVQNAVDQAEAAAAVIAGGSEPYDPKPW
FWSDQYDVKLQIAGFNLGYDETLLRPGAREGAHSIWYFKEGLLIAVDAINDAKAYVTGKKLLESGTNPDRSILADPSGDL
KRLLG

Sequences:

>Translated_405_residues
MTDRLVIIGAGQAGFAMAAKLRALKDTRPITLIGAEDVAPYQRPPLSKKYLLGEMAFDRLLFRDEHWYGDNDVDLRLSTW
AEQIKPDSKQVLLQDGSVLDYGTLALATGSTPRRLPAAIGGDLEGVYVARDKRDADLLAEEMRPGRRVLIIGGGYIGLEA
AAVARHRGLEVTVIEMADRILQRVAAKETADIMRGIHESHDVVIREKTGLKHLVGKDGRVTGAALSDGSVIDIDFAVVGI
GVVPNDLLAKEAGLEVANGIVVDEFARTSDPAIFAAGDCAALPWQGGRIRLESVQNAVDQAEAAAAVIAGGSEPYDPKPW
FWSDQYDVKLQIAGFNLGYDETLLRPGAREGAHSIWYFKEGLLIAVDAINDAKAYVTGKKLLESGTNPDRSILADPSGDL
KRLLG
>Mature_404_residues
TDRLVIIGAGQAGFAMAAKLRALKDTRPITLIGAEDVAPYQRPPLSKKYLLGEMAFDRLLFRDEHWYGDNDVDLRLSTWA
EQIKPDSKQVLLQDGSVLDYGTLALATGSTPRRLPAAIGGDLEGVYVARDKRDADLLAEEMRPGRRVLIIGGGYIGLEAA
AVARHRGLEVTVIEMADRILQRVAAKETADIMRGIHESHDVVIREKTGLKHLVGKDGRVTGAALSDGSVIDIDFAVVGIG
VVPNDLLAKEAGLEVANGIVVDEFARTSDPAIFAAGDCAALPWQGGRIRLESVQNAVDQAEAAAAVIAGGSEPYDPKPWF
WSDQYDVKLQIAGFNLGYDETLLRPGAREGAHSIWYFKEGLLIAVDAINDAKAYVTGKKLLESGTNPDRSILADPSGDLK
RLLG

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=374, Percent_Identity=27.2727272727273, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI21389617, Length=414, Percent_Identity=26.5700483091787, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI226437568, Length=374, Percent_Identity=27.2727272727273, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4757732, Length=335, Percent_Identity=28.0597014925373, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI22202629, Length=335, Percent_Identity=28.0597014925373, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=36.9727047146402, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789065, Length=285, Percent_Identity=29.8245614035088, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=31.6901408450704, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786307, Length=239, Percent_Identity=30.5439330543933, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=382, Percent_Identity=29.8429319371728, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24639257, Length=360, Percent_Identity=26.9444444444444, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI281359715, Length=360, Percent_Identity=26.9444444444444, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281359713, Length=360, Percent_Identity=26.9444444444444, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639250, Length=360, Percent_Identity=26.9444444444444, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18543267, Length=360, Percent_Identity=26.9444444444444, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24639252, Length=360, Percent_Identity=26.9444444444444, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24585130, Length=403, Percent_Identity=22.5806451612903, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28573993, Length=360, Percent_Identity=26.9444444444444, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24581020, Length=360, Percent_Identity=26.9444444444444, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43646; Mature: 43515

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRLVIIGAGQAGFAMAAKLRALKDTRPITLIGAEDVAPYQRPPLSKKYLLGEMAFDRL
CCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
LFRDEHWYGDNDVDLRLSTWAEQIKPDSKQVLLQDGSVLDYGTLALATGSTPRRLPAAIG
HHCCCCCCCCCCEEEEHHHHHHHHCCCCCEEEEECCCEEECCEEEEECCCCCCCCCHHHC
GDLEGVYVARDKRDADLLAEEMRPGRRVLIIGGGYIGLEAAAVARHRGLEVTVIEMADRI
CCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHCCCEEEHHHHHHHH
LQRVAAKETADIMRGIHESHDVVIREKTGLKHLVGKDGRVTGAALSDGSVIDIDFAVVGI
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCEEEEEEEEEEE
GVVPNDLLAKEAGLEVANGIVVDEFARTSDPAIFAAGDCAALPWQGGRIRLESVQNAVDQ
CCCCCHHHHHHCCCHHHCCEEEEHHHCCCCCEEEECCCCEECCCCCCEEEHHHHHHHHHH
AEAAAAVIAGGSEPYDPKPWFWSDQYDVKLQIAGFNLGYDETLLRPGAREGAHSIWYFKE
HHHHEEEEECCCCCCCCCCCEECCCCEEEEEEEEECCCCCHHHHCCCCCCCCCEEEEEEC
GLLIAVDAINDAKAYVTGKKLLESGTNPDRSILADPSGDLKRLLG
CEEEEEECCCCCHHEEEHHHHHHCCCCCCCCEEECCCHHHHHHCC
>Mature Secondary Structure 
TDRLVIIGAGQAGFAMAAKLRALKDTRPITLIGAEDVAPYQRPPLSKKYLLGEMAFDRL
CCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
LFRDEHWYGDNDVDLRLSTWAEQIKPDSKQVLLQDGSVLDYGTLALATGSTPRRLPAAIG
HHCCCCCCCCCCEEEEHHHHHHHHCCCCCEEEEECCCEEECCEEEEECCCCCCCCCHHHC
GDLEGVYVARDKRDADLLAEEMRPGRRVLIIGGGYIGLEAAAVARHRGLEVTVIEMADRI
CCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHCCCEEEHHHHHHHH
LQRVAAKETADIMRGIHESHDVVIREKTGLKHLVGKDGRVTGAALSDGSVIDIDFAVVGI
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCEEEEEEEEEEE
GVVPNDLLAKEAGLEVANGIVVDEFARTSDPAIFAAGDCAALPWQGGRIRLESVQNAVDQ
CCCCCHHHHHHCCCHHHCCEEEEHHHCCCCCEEEECCCCEECCCCCCEEEHHHHHHHHHH
AEAAAAVIAGGSEPYDPKPWFWSDQYDVKLQIAGFNLGYDETLLRPGAREGAHSIWYFKE
HHHHEEEEECCCCCCCCCCCEECCCCEEEEEEEEECCCCCHHHHCCCCCCCCCEEEEEEC
GLLIAVDAINDAKAYVTGKKLLESGTNPDRSILADPSGDLKRLLG
CEEEEEECCCCCHHEEEHHHHHHCCCCCCCCEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]