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The map label for this gene is gfo [H]

Identifier: 209548587

GI number: 209548587

Start: 1005193

End: 1006308

Strand: Direct

Name: gfo [H]

Synonym: Rleg2_0984

Alternate gene names: 209548587

Gene position: 1005193-1006308 (Clockwise)

Preceding gene: 209548578

Following gene: 209548588

Centisome position: 22.15

GC content: 61.2

Gene sequence:

>1116_bases
ATGAAGCCACTCGGCATCGGTTTGATCGGCACCGGTTATATGGGCAAGTGCCACGCGCTGGCATGGAATGCGGTGAAGAC
GGTGTTCGGCGATGTCGAGCGTCCGCGTCTCGTGCATCTGGCCGAAGCCAATGCCGGGCTTGCTGAGGCGCGAGCCGGTG
AATTCGGCTTCGAGAAGGCAACGGCCGACTGGCGGGCGCTGATCGCCGACCCCGAGGTCGATGTCGTCTCGGTCACCACA
CCTAATCAGTTCCATGCCGAGATGGCCATTGCAGCGCTTGAAGCCGGCAAACACGTCTGGTGTGAAAAGCCGATGGCGCC
GGCCTATGCCGATGCCGAGCGCATGCTGGAAACGGCGAAGCGTTCCGGCAAGGTTGCCGCCCTTGGCTATAATTACATCC
AGAATCCCGTCATACGGCACATCAAGACGCTTGTCGGCGAGGGCGCCATCGGCACGGTCAACCATATCCGCGTCGAGATG
GACGAGGATTTCATGGCGGATGCCGATGTCTTCTTCTATTGGAAGAGCGAGCTTTCCGCCGGCTACGGCGCGCTCGACGA
TTTCGCCGTGCACCCGCTGTCGCTGCTCTGGTATCTCTTCGGCCATGTCGAGGCGGTCATTACGGATATGGTGAAGCCCT
ATGCCGACCGCCCGCTGAGCGAGGGTGGTCGCCGTGCCGTCGAAAATCACGATGCCGCCAACGTGCTGTTGCGGCTTGGC
GGCGGCATCTCCGCCGTGTTGATGGCCAACCGCGCCGCCTGGGGCCGCAAGGGCCGCATCGCCCTGCAGATTTATGGTTC
GCAAGGATCGATCGTCTATGACCAGGAGCGGATGAACGAATTCGAGCTCTATCAGGCCGAGGGTCGCGGCTCCGAACAGG
GTTTCCGCAAGATACTGGCGGCGCCCGCCCATCGGCCTTATGATCGCTTTATCCCGGCGCCCGGTCACGGTCTCGGCTTC
AACGATCTAAAGATCATCGAATGCCGCGAGCTGATCCGGGCAATATCGGGCGAGCCGTCGTCGATCGTGACATTTAAAGA
CGGTCTCAGGATAGAGAAGTCGGTGCATGCCATGGCACAGTCCTTCCACGAGCGTCGCTGGATCGAGATCGGCTGA

Upstream 100 bases:

>100_bases
CAATTCTCCCGAAAAATGGTGCCGATGACCGTGGATAGGTCTCTTGTCAAAATGATCCAAATATTCCAAAAATCCGCCCG
CAGCTCTGGAGGAAACGGAA

Downstream 100 bases:

>100_bases
AGGTAAGAAGCCGCTTCCGTTGACGGAGCATAGGGCAGGGGATAGGCGTTGGCATGAGTGTCGGGCCTCGTTTCAGGAGT
GAGATCGTGACGGATAGACT

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: GFOR [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKPLGIGLIGTGYMGKCHALAWNAVKTVFGDVERPRLVHLAEANAGLAEARAGEFGFEKATADWRALIADPEVDVVSVTT
PNQFHAEMAIAALEAGKHVWCEKPMAPAYADAERMLETAKRSGKVAALGYNYIQNPVIRHIKTLVGEGAIGTVNHIRVEM
DEDFMADADVFFYWKSELSAGYGALDDFAVHPLSLLWYLFGHVEAVITDMVKPYADRPLSEGGRRAVENHDAANVLLRLG
GGISAVLMANRAAWGRKGRIALQIYGSQGSIVYDQERMNEFELYQAEGRGSEQGFRKILAAPAHRPYDRFIPAPGHGLGF
NDLKIIECRELIRAISGEPSSIVTFKDGLRIEKSVHAMAQSFHERRWIEIG

Sequences:

>Translated_371_residues
MKPLGIGLIGTGYMGKCHALAWNAVKTVFGDVERPRLVHLAEANAGLAEARAGEFGFEKATADWRALIADPEVDVVSVTT
PNQFHAEMAIAALEAGKHVWCEKPMAPAYADAERMLETAKRSGKVAALGYNYIQNPVIRHIKTLVGEGAIGTVNHIRVEM
DEDFMADADVFFYWKSELSAGYGALDDFAVHPLSLLWYLFGHVEAVITDMVKPYADRPLSEGGRRAVENHDAANVLLRLG
GGISAVLMANRAAWGRKGRIALQIYGSQGSIVYDQERMNEFELYQAEGRGSEQGFRKILAAPAHRPYDRFIPAPGHGLGF
NDLKIIECRELIRAISGEPSSIVTFKDGLRIEKSVHAMAQSFHERRWIEIG
>Mature_371_residues
MKPLGIGLIGTGYMGKCHALAWNAVKTVFGDVERPRLVHLAEANAGLAEARAGEFGFEKATADWRALIADPEVDVVSVTT
PNQFHAEMAIAALEAGKHVWCEKPMAPAYADAERMLETAKRSGKVAALGYNYIQNPVIRHIKTLVGEGAIGTVNHIRVEM
DEDFMADADVFFYWKSELSAGYGALDDFAVHPLSLLWYLFGHVEAVITDMVKPYADRPLSEGGRRAVENHDAANVLLRLG
GGISAVLMANRAAWGRKGRIALQIYGSQGSIVYDQERMNEFELYQAEGRGSEQGFRKILAAPAHRPYDRFIPAPGHGLGF
NDLKIIECRELIRAISGEPSSIVTFKDGLRIEKSVHAMAQSFHERRWIEIG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=210, Percent_Identity=30, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081947, Length=163, Percent_Identity=30.0613496932515, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082405, Length=145, Percent_Identity=28.9655172413793, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008354
- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.99.28 [H]

Molecular weight: Translated: 40861; Mature: 40861

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLGIGLIGTGYMGKCHALAWNAVKTVFGDVERPRLVHLAEANAGLAEARAGEFGFEKA
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCCCCCHHC
TADWRALIADPEVDVVSVTTPNQFHAEMAIAALEAGKHVWCEKPMAPAYADAERMLETAK
CCCHHEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHH
RSGKVAALGYNYIQNPVIRHIKTLVGEGAIGTVNHIRVEMDEDFMADADVFFYWKSELSA
HCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCC
GYGALDDFAVHPLSLLWYLFGHVEAVITDMVKPYADRPLSEGGRRAVENHDAANVLLRLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHC
GGISAVLMANRAAWGRKGRIALQIYGSQGSIVYDQERMNEFELYQAEGRGSEQGFRKILA
CCCEEEHHHHHHCCCCCCEEEEEEECCCCCEEECHHHCCCEEEEECCCCCCHHHHHHHHH
APAHRPYDRFIPAPGHGLGFNDLKIIECRELIRAISGEPSSIVTFKDGLRIEKSVHAMAQ
CCCCCCHHHCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
SFHERRWIEIG
HHHHCCCEECC
>Mature Secondary Structure
MKPLGIGLIGTGYMGKCHALAWNAVKTVFGDVERPRLVHLAEANAGLAEARAGEFGFEKA
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCCCCCHHC
TADWRALIADPEVDVVSVTTPNQFHAEMAIAALEAGKHVWCEKPMAPAYADAERMLETAK
CCCHHEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHH
RSGKVAALGYNYIQNPVIRHIKTLVGEGAIGTVNHIRVEMDEDFMADADVFFYWKSELSA
HCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCC
GYGALDDFAVHPLSLLWYLFGHVEAVITDMVKPYADRPLSEGGRRAVENHDAANVLLRLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHC
GGISAVLMANRAAWGRKGRIALQIYGSQGSIVYDQERMNEFELYQAEGRGSEQGFRKILA
CCCEEEHHHHHHCCCCCCEEEEEEECCCCCEEECHHHCCCEEEEECCCCCCHHHHHHHHH
APAHRPYDRFIPAPGHGLGFNDLKIIECRELIRAISGEPSSIVTFKDGLRIEKSVHAMAQ
CCCCCCHHHCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
SFHERRWIEIG
HHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1537789; 8472911; 8994968 [H]