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The map label for this gene is tam

Identifier: 209548430

GI number: 209548430

Start: 844650

End: 845420

Strand: Reverse

Name: tam

Synonym: Rleg2_0827

Alternate gene names: 209548430

Gene position: 845420-844650 (Counterclockwise)

Preceding gene: 209548431

Following gene: 209548429

Centisome position: 18.63

GC content: 63.94

Gene sequence:

>771_bases
ATGGCATGGTCCGCCAGCCAATATGTGAAATTCGAGGACGAACGCACACGACCGGCACGTGACCTTCTGGCGCAAGTGCC
GCTGCAAAAGATCCGCCGCGCCGTCGATCTCGGCTGCGGGCCGGGCAATTCGACCGAGCTGATCATCGAGCGCTATGGGG
CCGACGGCGTCTCCGGCCTCGACAGCGACATGAACATGCTGGAGGCGGCCCGCAAGCGGCTTCCAGGCACGGCCTTCGTC
GAAGCCGATCTCGGCAGCTGGCAGCCGATGGAGCCGGCCGACCTGCTGTTTGCCAATGCCGTCTTCCAATGGCTGCCCGA
TCATCTCGATATTTTCGACCGGCTGATGGACGGGCTTTCCCCGGGCGGCGTGCTCGCGGTGCAGATGCCCGACAATCTCG
GCGAACCCTCGCATCTGGCGATGGAGGAGGCCGCCCATGCCGGCCCCTGGAAGACAGCCTTCGAGGCGAAGAGCGTGCGC
CGCAAGGGGCTGCCCGCGCCATCGGCCTATTATTCCCGTCTGATCACCAAGGCGTCGCGCGTCGACGTCTGGCATACGAT
CTACAATCACCCGATGGCGGATGCGGCCGCGATCGTCGAATGGGTGAAGGGAACCGGGCTGATGCCCTATCTCGCCCATG
CCGGCGAGAGATATCGCGAGGCGTTTCTGGCGGATTATCTGCAGCGAGTGGAAAAGGCCTATCCGAAGCTGTCGGACGGG
CGGGTGCTGCTACGCTTTCCGCGGATTTTCATGGTGGCGGTGAAGGGGTAA

Upstream 100 bases:

>100_bases
GCATACTTGGCTTGAGATCATCGAGCCCGACGGCCGCAACCTGGACGAAATCCGCATCCGCCACATGGAAAATGTGGCCT
GATATCAGGGAGTAAACGCC

Downstream 100 bases:

>100_bases
CCGGTTGCTGCGGGCGGGCTCGGTTCGGACCGCGGCTGCCCCTCACCCTAGCCCTCTCCCCGTTCTGACGGGGAGAGGGG
ACGTGCCCTGCGAGAGCTTA

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAWSASQYVKFEDERTRPARDLLAQVPLQKIRRAVDLGCGPGNSTELIIERYGADGVSGLDSDMNMLEAARKRLPGTAFV
EADLGSWQPMEPADLLFANAVFQWLPDHLDIFDRLMDGLSPGGVLAVQMPDNLGEPSHLAMEEAAHAGPWKTAFEAKSVR
RKGLPAPSAYYSRLITKASRVDVWHTIYNHPMADAAAIVEWVKGTGLMPYLAHAGERYREAFLADYLQRVEKAYPKLSDG
RVLLRFPRIFMVAVKG

Sequences:

>Translated_256_residues
MAWSASQYVKFEDERTRPARDLLAQVPLQKIRRAVDLGCGPGNSTELIIERYGADGVSGLDSDMNMLEAARKRLPGTAFV
EADLGSWQPMEPADLLFANAVFQWLPDHLDIFDRLMDGLSPGGVLAVQMPDNLGEPSHLAMEEAAHAGPWKTAFEAKSVR
RKGLPAPSAYYSRLITKASRVDVWHTIYNHPMADAAAIVEWVKGTGLMPYLAHAGERYREAFLADYLQRVEKAYPKLSDG
RVLLRFPRIFMVAVKG
>Mature_255_residues
AWSASQYVKFEDERTRPARDLLAQVPLQKIRRAVDLGCGPGNSTELIIERYGADGVSGLDSDMNMLEAARKRLPGTAFVE
ADLGSWQPMEPADLLFANAVFQWLPDHLDIFDRLMDGLSPGGVLAVQMPDNLGEPSHLAMEEAAHAGPWKTAFEAKSVRR
KGLPAPSAYYSRLITKASRVDVWHTIYNHPMADAAAIVEWVKGTGLMPYLAHAGERYREAFLADYLQRVEKAYPKLSDGR
VLLRFPRIFMVAVKG

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family

Homologues:

Organism=Escherichia coli, GI1787798, Length=251, Percent_Identity=46.2151394422311, Blast_Score=233, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TAM_RHILW (B5ZUL2)

Other databases:

- EMBL:   CP001191
- RefSeq:   YP_002280347.1
- ProteinModelPortal:   B5ZUL2
- SMR:   B5ZUL2
- GeneID:   6979545
- GenomeReviews:   CP001191_GR
- KEGG:   rlt:Rleg2_0827
- HOGENOM:   HBG328684
- OMA:   WETTYVH
- ProtClustDB:   PRK01683
- GO:   GO:0005737
- HAMAP:   MF_00560
- InterPro:   IPR013216
- InterPro:   IPR023149

Pfam domain/function: PF08241 Methyltransf_11

EC number: =2.1.1.144

Molecular weight: Translated: 28471; Mature: 28340

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWSASQYVKFEDERTRPARDLLAQVPLQKIRRAVDLGCGPGNSTELIIERYGADGVSGL
CCCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEHEEECCCCCCCCC
DSDMNMLEAARKRLPGTAFVEADLGSWQPMEPADLLFANAVFQWLPDHLDIFDRLMDGLS
CHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
PGGVLAVQMPDNLGEPSHLAMEEAAHAGPWKTAFEAKSVRRKGLPAPSAYYSRLITKASR
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VDVWHTIYNHPMADAAAIVEWVKGTGLMPYLAHAGERYREAFLADYLQRVEKAYPKLSDG
HHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVLLRFPRIFMVAVKG
EEEEEECCEEEEEECC
>Mature Secondary Structure 
AWSASQYVKFEDERTRPARDLLAQVPLQKIRRAVDLGCGPGNSTELIIERYGADGVSGL
CCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEHEEECCCCCCCCC
DSDMNMLEAARKRLPGTAFVEADLGSWQPMEPADLLFANAVFQWLPDHLDIFDRLMDGLS
CHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
PGGVLAVQMPDNLGEPSHLAMEEAAHAGPWKTAFEAKSVRRKGLPAPSAYYSRLITKASR
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VDVWHTIYNHPMADAAAIVEWVKGTGLMPYLAHAGERYREAFLADYLQRVEKAYPKLSDG
HHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVLLRFPRIFMVAVKG
EEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA