Click here to switch to the map view.

The map label for this gene is cysH [H]

Identifier: 209548375

GI number: 209548375

Start: 789903

End: 790661

Strand: Reverse

Name: cysH [H]

Synonym: Rleg2_0771

Alternate gene names: 209548375

Gene position: 790661-789903 (Counterclockwise)

Preceding gene: 209548377

Following gene: 209548374

Centisome position: 17.42

GC content: 64.56

Gene sequence:

>759_bases
ATGACTGTTATCAATACGATTGCGGAAGAAGGGCCTATTGCCGAAGCCGAGGCGCTGAACGACAAGCTGGCAGGTCTCGA
CCTGGCCGGACGTCTGTCGCTGGTCTCCGGCCTCGGCGGCCGCGTGGTGTTCACCACCTCGCTCGGCATCGAGGACCAGG
TGATATCAGCCGAGATCGGCACGCATCGCCTGCCGATCGACGTCGCGACGCTGCAGACCGGCCGGCTGTTTGCCGAGACG
CTGTCGCTGATTACCGAGACGGAAAGCCAGTACGACATCAAGATCCAGCGCTACGAGCCCGAGAAGGCCGATATTGACGC
CTATGCGGCGCAATATGGCCTCAACGGTTTCTACGAGAGCGTCGAAGCCAGGCATGCCTGTTGCGGCGTGCGCAAGCTGA
AGCCGCTTGCGCGTGCACTCGAAGGGGCGACGATCTGGATCACCGGCCTGCGCCGCGGCCAATCGGCCAACCGCGCCGAA
ACGCCCTTTGCCGAATACGACGCCGAACGGCACCTTTTGAAGGTCAATCCGCTCGCCGATTGGGACCTGGAGGCGATCAA
GGCCTTCGTGTCCGACAACGGCGTGCCGGTCAATCCGCTGCATGCCCGCGGCTATCCCTCGATCGGCTGCGAGCCCTGCA
CCCGGGCGATCAAGCCCGGTGAGCCGGAACGCGCCGGCCGCTGGTGGTGGGAGCAGGACGAGACGCGCGAATGCGGCCTG
CATGTCGCCGAAGAGGCCGCCGTCATCGCCGCACAATAA

Upstream 100 bases:

>100_bases
GAGAGATATTTGCTCCTCCGGCCGCAGCTTTCGAAATCCCTTTTTCTGTCCGATCCAGGATTGAACTGCGATACTCCCGT
CAACAAAGGACAGGATTCTC

Downstream 100 bases:

>100_bases
TCCACTTCACGGCTTAACGAACCCGGGCGCGGCCACCGCCGCAGCCCTGGGAAAGCCAGCATCAGGAACTGCTTTGCGGT
GAACCGCAAAGGATCGACAG

Product: adenylylsulfate reductase, thioredoxin dependent

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTVINTIAEEGPIAEAEALNDKLAGLDLAGRLSLVSGLGGRVVFTTSLGIEDQVISAEIGTHRLPIDVATLQTGRLFAET
LSLITETESQYDIKIQRYEPEKADIDAYAAQYGLNGFYESVEARHACCGVRKLKPLARALEGATIWITGLRRGQSANRAE
TPFAEYDAERHLLKVNPLADWDLEAIKAFVSDNGVPVNPLHARGYPSIGCEPCTRAIKPGEPERAGRWWWEQDETRECGL
HVAEEAAVIAAQ

Sequences:

>Translated_252_residues
MTVINTIAEEGPIAEAEALNDKLAGLDLAGRLSLVSGLGGRVVFTTSLGIEDQVISAEIGTHRLPIDVATLQTGRLFAET
LSLITETESQYDIKIQRYEPEKADIDAYAAQYGLNGFYESVEARHACCGVRKLKPLARALEGATIWITGLRRGQSANRAE
TPFAEYDAERHLLKVNPLADWDLEAIKAFVSDNGVPVNPLHARGYPSIGCEPCTRAIKPGEPERAGRWWWEQDETRECGL
HVAEEAAVIAAQ
>Mature_251_residues
TVINTIAEEGPIAEAEALNDKLAGLDLAGRLSLVSGLGGRVVFTTSLGIEDQVISAEIGTHRLPIDVATLQTGRLFAETL
SLITETESQYDIKIQRYEPEKADIDAYAAQYGLNGFYESVEARHACCGVRKLKPLARALEGATIWITGLRRGQSANRAET
PFAEYDAERHLLKVNPLADWDLEAIKAFVSDNGVPVNPLHARGYPSIGCEPCTRAIKPGEPERAGRWWWEQDETRECGLH
VAEEAAVIAAQ

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=244, Percent_Identity=30.7377049180328, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6325425, Length=189, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 27526; Mature: 27395

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVINTIAEEGPIAEAEALNDKLAGLDLAGRLSLVSGLGGRVVFTTSLGIEDQVISAEIG
CCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCC
THRLPIDVATLQTGRLFAETLSLITETESQYDIKIQRYEPEKADIDAYAAQYGLNGFYES
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHH
VEARHACCGVRKLKPLARALEGATIWITGLRRGQSANRAETPFAEYDAERHLLKVNPLAD
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCEEEECCCCC
WDLEAIKAFVSDNGVPVNPLHARGYPSIGCEPCTRAIKPGEPERAGRWWWEQDETRECGL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHCC
HVAEEAAVIAAQ
EECCCCEEEECC
>Mature Secondary Structure 
TVINTIAEEGPIAEAEALNDKLAGLDLAGRLSLVSGLGGRVVFTTSLGIEDQVISAEIG
CHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCC
THRLPIDVATLQTGRLFAETLSLITETESQYDIKIQRYEPEKADIDAYAAQYGLNGFYES
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHH
VEARHACCGVRKLKPLARALEGATIWITGLRRGQSANRAETPFAEYDAERHLLKVNPLAD
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCEEEECCCCC
WDLEAIKAFVSDNGVPVNPLHARGYPSIGCEPCTRAIKPGEPERAGRWWWEQDETRECGL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHCC
HVAEEAAVIAAQ
EECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10464198; 11481430 [H]