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The map label for this gene is clcA [H]

Identifier: 209548377

GI number: 209548377

Start: 791546

End: 792880

Strand: Reverse

Name: clcA [H]

Synonym: Rleg2_0773

Alternate gene names: 209548377

Gene position: 792880-791546 (Counterclockwise)

Preceding gene: 209548380

Following gene: 209548375

Centisome position: 17.47

GC content: 65.32

Gene sequence:

>1335_bases
ATGCCAGTGTTGTATCGCAAGTCCAGACTTCTTCGCAGATCCCGTGTTCTCTGGGGTTCCCTCGCGCTCTGGCGCCCGCG
GCTGATCTTCTGGTTCGGCGCTTTTGCGATCGGCATCATCAGCGTCGGTTTCGCCAGGCTGGCCGATCTTGCCCAACGCG
CCTTTGCCGGCCTGACCACATCGGGCGAATGGGGCTTCCTGCTGCCGCTCGTCATTACGCCGCTCGGTTTCATGCTGTCG
GCCTATCTCGCCGCACGGTTTTTCCCGAACGCGCAGGGCAGCGGTATTCCGCAGGCGATCGCCGCGCGGCATCTGCGCGA
TCCGGAGGACCGAACCCGTCTGCTGTCGCTGCGGCTGGTCTTCGGCAAGATCGTGCTGACGGTGCTCGGCCTCCTCAGCG
GCGCATCGATCGGCCGCGAAGGACCGACCGTGCAGGTGGGCGCTTCGATCATGCTGGCGGTCGCGCGTTTCGGCGGCATG
GCACAGGCCCGCGGGCTGATTCTTGCCGGTTCGGCTGCCGGCATCGCCGCTGCCTTCAATACGCCGCTCGCCGGCATCGT
CTTTGCGATCGAGGAGATGAGCCGGACCTATGAATCGCGTGCCAACGGCCTGGTTCTGACCGCCGTCATCCTGTCCGGCC
TTGCCGCCCTCGGGCTTGCCGGCAGCTACAATTATTTCGGCTCTACTGCGGTGGCGCCAGTATCGCTGCGCGACTGGGAA
CTGGTGCTCGTCTGCGGCATCGGCGGCGGCGCGCTCGGTGCGGTTTTCAGCGGGCTTGCGCTGCATGCCGGCCAGCATGT
GCGCCGCTTTGCACAGCCACAACCGCTGCGGCGGATGGTGATGCTGGCAACCGTCTGCGGCCTTGCCGTCGCCGTTATCG
GCATCCTGTCGGGTGGCACGACTTTCGGAACCGGCTACGATCAGGCGAAAGGCGCGGTCGAGGGCAATCCCCTGCCCCTG
CTCTTCTTCGTCGAAAAGCTTGTTGCAGGCTTCCTGTCGATGATTTCGGGAATCCCGGGCGGCATCTTCGCGCCCTCACT
CGCCATCGGCGCCGGCTTCGGCAGCACGGTCGGCCAACTGATGGGCAGCGGCATCGCGCTTGCGGCAATCCTCGGCATGG
CCGGATATTTCGCCGGCGTTGTGCAGGCACCGATGACAGCCTTCGTCATCATCCTCGAGATGACCGGCGATCACCAGGCC
GTCATCCCGATCATGGCGGTATCGATGATCGGCTACATCACCTCGCGCCTGCTGTCGCGCGAGCCGCTCTATCACGGTCT
GTCGCGCGTGTTCATTGCCGCGGCGATCAGGGCGCGGCGGGCCGAAGCAGAATGA

Upstream 100 bases:

>100_bases
GCGATCGGAGACCGGCAAACAGTGCTGGATCTGGAAGAAAGAGGTGGCCCCTTCCCCGCAAACCGGTTTAGGAAGAGGTC
CTGTCCAACATCGCTCCGGC

Downstream 100 bases:

>100_bases
ATTCGGGGAAGAAGCCCCTCAGGCGAGCAGCCGGGTGACTTCGGCGCCGTGCCTTGGGCCGACGGCGTCGGCGATGGTTG
TCGAGAACAGCACCTTGCGG

Product: chloride channel core protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPVLYRKSRLLRRSRVLWGSLALWRPRLIFWFGAFAIGIISVGFARLADLAQRAFAGLTTSGEWGFLLPLVITPLGFMLS
AYLAARFFPNAQGSGIPQAIAARHLRDPEDRTRLLSLRLVFGKIVLTVLGLLSGASIGREGPTVQVGASIMLAVARFGGM
AQARGLILAGSAAGIAAAFNTPLAGIVFAIEEMSRTYESRANGLVLTAVILSGLAALGLAGSYNYFGSTAVAPVSLRDWE
LVLVCGIGGGALGAVFSGLALHAGQHVRRFAQPQPLRRMVMLATVCGLAVAVIGILSGGTTFGTGYDQAKGAVEGNPLPL
LFFVEKLVAGFLSMISGIPGGIFAPSLAIGAGFGSTVGQLMGSGIALAAILGMAGYFAGVVQAPMTAFVIILEMTGDHQA
VIPIMAVSMIGYITSRLLSREPLYHGLSRVFIAAAIRARRAEAE

Sequences:

>Translated_444_residues
MPVLYRKSRLLRRSRVLWGSLALWRPRLIFWFGAFAIGIISVGFARLADLAQRAFAGLTTSGEWGFLLPLVITPLGFMLS
AYLAARFFPNAQGSGIPQAIAARHLRDPEDRTRLLSLRLVFGKIVLTVLGLLSGASIGREGPTVQVGASIMLAVARFGGM
AQARGLILAGSAAGIAAAFNTPLAGIVFAIEEMSRTYESRANGLVLTAVILSGLAALGLAGSYNYFGSTAVAPVSLRDWE
LVLVCGIGGGALGAVFSGLALHAGQHVRRFAQPQPLRRMVMLATVCGLAVAVIGILSGGTTFGTGYDQAKGAVEGNPLPL
LFFVEKLVAGFLSMISGIPGGIFAPSLAIGAGFGSTVGQLMGSGIALAAILGMAGYFAGVVQAPMTAFVIILEMTGDHQA
VIPIMAVSMIGYITSRLLSREPLYHGLSRVFIAAAIRARRAEAE
>Mature_443_residues
PVLYRKSRLLRRSRVLWGSLALWRPRLIFWFGAFAIGIISVGFARLADLAQRAFAGLTTSGEWGFLLPLVITPLGFMLSA
YLAARFFPNAQGSGIPQAIAARHLRDPEDRTRLLSLRLVFGKIVLTVLGLLSGASIGREGPTVQVGASIMLAVARFGGMA
QARGLILAGSAAGIAAAFNTPLAGIVFAIEEMSRTYESRANGLVLTAVILSGLAALGLAGSYNYFGSTAVAPVSLRDWEL
VLVCGIGGGALGAVFSGLALHAGQHVRRFAQPQPLRRMVMLATVCGLAVAVIGILSGGTTFGTGYDQAKGAVEGNPLPLL
FFVEKLVAGFLSMISGIPGGIFAPSLAIGAGFGSTVGQLMGSGIALAAILGMAGYFAGVVQAPMTAFVIILEMTGDHQAV
IPIMAVSMIGYITSRLLSREPLYHGLSRVFIAAAIRARRAEAE

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=407, Percent_Identity=30.2211302211302, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081943, Length=322, Percent_Identity=30.1242236024845, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 46347; Mature: 46215

Theoretical pI: Translated: 11.25; Mature: 11.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLYRKSRLLRRSRVLWGSLALWRPRLIFWFGAFAIGIISVGFARLADLAQRAFAGLTT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGEWGFLLPLVITPLGFMLSAYLAARFFPNAQGSGIPQAIAARHLRDPEDRTRLLSLRLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
FGKIVLTVLGLLSGASIGREGPTVQVGASIMLAVARFGGMAQARGLILAGSAAGIAAAFN
HHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHC
TPLAGIVFAIEEMSRTYESRANGLVLTAVILSGLAALGLAGSYNYFGSTAVAPVSLRDWE
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCE
LVLVCGIGGGALGAVFSGLALHAGQHVRRFAQPQPLRRMVMLATVCGLAVAVIGILSGGT
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFGTGYDQAKGAVEGNPLPLLFFVEKLVAGFLSMISGIPGGIFAPSLAIGAGFGSTVGQL
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHCCCHHHHHHH
MGSGIALAAILGMAGYFAGVVQAPMTAFVIILEMTGDHQAVIPIMAVSMIGYITSRLLSR
HCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
EPLYHGLSRVFIAAAIRARRAEAE
CHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVLYRKSRLLRRSRVLWGSLALWRPRLIFWFGAFAIGIISVGFARLADLAQRAFAGLTT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGEWGFLLPLVITPLGFMLSAYLAARFFPNAQGSGIPQAIAARHLRDPEDRTRLLSLRLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
FGKIVLTVLGLLSGASIGREGPTVQVGASIMLAVARFGGMAQARGLILAGSAAGIAAAFN
HHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHC
TPLAGIVFAIEEMSRTYESRANGLVLTAVILSGLAALGLAGSYNYFGSTAVAPVSLRDWE
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCE
LVLVCGIGGGALGAVFSGLALHAGQHVRRFAQPQPLRRMVMLATVCGLAVAVIGILSGGT
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFGTGYDQAKGAVEGNPLPLLFFVEKLVAGFLSMISGIPGGIFAPSLAIGAGFGSTVGQL
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHCCCHHHHHHH
MGSGIALAAILGMAGYFAGVVQAPMTAFVIILEMTGDHQAVIPIMAVSMIGYITSRLLSR
HCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
EPLYHGLSRVFIAAAIRARRAEAE
CHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA