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The map label for this gene is cysD [H]

Identifier: 209548374

GI number: 209548374

Start: 788836

End: 789789

Strand: Reverse

Name: cysD [H]

Synonym: Rleg2_0770

Alternate gene names: 209548374

Gene position: 789789-788836 (Counterclockwise)

Preceding gene: 209548375

Following gene: 209548373

Centisome position: 17.4

GC content: 63.42

Gene sequence:

>954_bases
ATGCCCGATAGCCGTCCGGATACGGAACTCAGCAATCCCCAGAGCGCCAAGGCGCCACTCGACCCGCATCTGAAGGCGCT
GGAAAACGAATCCATCCACATCTTCCGCGAGGTTGCCGCCGAATTCGAGCGTCCCGTCATGCTCTATTCGATCGGCAAGG
ATTCCTCGGTGCTGCTGCATCTGGCGCGCAAGGCCTTCTATCCCGGCCGCGTGCCCTTCCCGCTCCTGCATGTGAACACC
GGCTGGAAATTCCGGGAGATGATCGCCTTCCGCGACGAAACCGCGAAGAAGTATGATCTCGACCTGATCGAGCACATCAA
TCCGCGCGGCGCGGCCGAAAACATCACGCCGTTCACCCATGGATCGGCGGCCTTCACCGACATCATGAAGACCGAGGGCC
TGCGCCAAGCGCTCGATGCCGGCCAGTTCGACGCCGCTTTCGGCGGTGCGCGGCGCGACGAGGAAGCCAGCCGCGCCAAG
GAGCGCATCTATTCCTTCCGCACGCCCGACCATCGCTGGGACCCGCGCAACCAGCGGCCGGAACTCTGGAACGTCTATAA
CGGCATGATCCGCAAGGGCGAGAGCGTGCGCGCCTTCCCGCTGTCGAACTGGACGGAGGTCGATATCTGGCGCTACATCC
AGGCCGAGGACATTCCGCTGGTGCCGCTCTATTACGCCAAGAAGCGGCCCTTCGTGGAGCGCGACGGCATGATGATCCTG
GCCGAGGATCCGCGCCTCGAACTGCTGCCCGGCGAAGTGCGCCAGGAGGGCATGATCCGCTTCCGCACGCTCGGCGACTT
CCCGCTGACCGGCGCCATCCGCTCACAGGCCACCACCCTCGAAGAGGTGATTGCCGAACTCGAAATCGCAACGGTGTCCG
AACGCCAGGGCCGCGCTATCGACCGCGACCAGTCCGGCTCCATGGAAAAGAAGAAGCGTGAAGGATATTTCTGA

Upstream 100 bases:

>100_bases
TTAACGAACCCGGGCGCGGCCACCGCCGCAGCCCTGGGAAAGCCAGCATCAGGAACTGCTTTGCGGTGAACCGCAAAGGA
TCGACAGTCTGGAGTAAAAC

Downstream 100 bases:

>100_bases
GATGACCGCAGCCCAAACCGCCGTCTCGGCCGCCACCGTTGTCAGCCTGCCCGCCGCCGAGCCGCAGAAGGCCCAGCGCG
ACACACGCCCGCTGCGCCTG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MPDSRPDTELSNPQSAKAPLDPHLKALENESIHIFREVAAEFERPVMLYSIGKDSSVLLHLARKAFYPGRVPFPLLHVNT
GWKFREMIAFRDETAKKYDLDLIEHINPRGAAENITPFTHGSAAFTDIMKTEGLRQALDAGQFDAAFGGARRDEEASRAK
ERIYSFRTPDHRWDPRNQRPELWNVYNGMIRKGESVRAFPLSNWTEVDIWRYIQAEDIPLVPLYYAKKRPFVERDGMMIL
AEDPRLELLPGEVRQEGMIRFRTLGDFPLTGAIRSQATTLEEVIAELEIATVSERQGRAIDRDQSGSMEKKKREGYF

Sequences:

>Translated_317_residues
MPDSRPDTELSNPQSAKAPLDPHLKALENESIHIFREVAAEFERPVMLYSIGKDSSVLLHLARKAFYPGRVPFPLLHVNT
GWKFREMIAFRDETAKKYDLDLIEHINPRGAAENITPFTHGSAAFTDIMKTEGLRQALDAGQFDAAFGGARRDEEASRAK
ERIYSFRTPDHRWDPRNQRPELWNVYNGMIRKGESVRAFPLSNWTEVDIWRYIQAEDIPLVPLYYAKKRPFVERDGMMIL
AEDPRLELLPGEVRQEGMIRFRTLGDFPLTGAIRSQATTLEEVIAELEIATVSERQGRAIDRDQSGSMEKKKREGYF
>Mature_316_residues
PDSRPDTELSNPQSAKAPLDPHLKALENESIHIFREVAAEFERPVMLYSIGKDSSVLLHLARKAFYPGRVPFPLLHVNTG
WKFREMIAFRDETAKKYDLDLIEHINPRGAAENITPFTHGSAAFTDIMKTEGLRQALDAGQFDAAFGGARRDEEASRAKE
RIYSFRTPDHRWDPRNQRPELWNVYNGMIRKGESVRAFPLSNWTEVDIWRYIQAEDIPLVPLYYAKKRPFVERDGMMILA
EDPRLELLPGEVRQEGMIRFRTLGDFPLTGAIRSQATTLEEVIAELEIATVSERQGRAIDRDQSGSMEKKKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=295, Percent_Identity=70.1694915254237, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36408; Mature: 36277

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSRPDTELSNPQSAKAPLDPHLKALENESIHIFREVAAEFERPVMLYSIGKDSSVLLH
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LARKAFYPGRVPFPLLHVNTGWKFREMIAFRDETAKKYDLDLIEHINPRGAAENITPFTH
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHCHHHHHHCCCCCCCCCCCCCCC
GSAAFTDIMKTEGLRQALDAGQFDAAFGGARRDEEASRAKERIYSFRTPDHRWDPRNQRP
CHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
ELWNVYNGMIRKGESVRAFPLSNWTEVDIWRYIQAEDIPLVPLYYAKKRPFVERDGMMIL
HHHHHHHHHHHCCCCEEEEECCCCCEEHHEEEEECCCCCEEEHHHHCCCCCCCCCCEEEE
AEDPRLELLPGEVRQEGMIRFRTLGDFPLTGAIRSQATTLEEVIAELEIATVSERQGRAI
ECCCCEEECCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DRDQSGSMEKKKREGYF
CCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
PDSRPDTELSNPQSAKAPLDPHLKALENESIHIFREVAAEFERPVMLYSIGKDSSVLLH
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LARKAFYPGRVPFPLLHVNTGWKFREMIAFRDETAKKYDLDLIEHINPRGAAENITPFTH
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHCHHHHHHCCCCCCCCCCCCCCC
GSAAFTDIMKTEGLRQALDAGQFDAAFGGARRDEEASRAKERIYSFRTPDHRWDPRNQRP
CHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
ELWNVYNGMIRKGESVRAFPLSNWTEVDIWRYIQAEDIPLVPLYYAKKRPFVERDGMMIL
HHHHHHHHHHHCCCCEEEEECCCCCEEHHEEEEECCCCCEEEHHHHCCCCCCCCCCEEEE
AEDPRLELLPGEVRQEGMIRFRTLGDFPLTGAIRSQATTLEEVIAELEIATVSERQGRAI
ECCCCEEECCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DRDQSGSMEKKKREGYF
CCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA