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The map label for this gene is rsmE [H]

Identifier: 209548049

GI number: 209548049

Start: 452844

End: 453581

Strand: Reverse

Name: rsmE [H]

Synonym: Rleg2_0442

Alternate gene names: 209548049

Gene position: 453581-452844 (Counterclockwise)

Preceding gene: 209548050

Following gene: 209548048

Centisome position: 10.0

GC content: 65.99

Gene sequence:

>738_bases
ATGCGCGCCAATTTCCGCATGCAACGGCTTTTCGTCGACGCGCCGCTTTCCAGCGGCGCCGCCCTCGAGGCGAATGCCGA
CCAGTTCAACTATCTCGCCAATGTGCTGAGGATGGAGGAAGGCGGGGAGGTCCTGCTCTTCAACGGCCGCGACGGCGAGT
GGAAGGCCACCCTCACCTTCCCCACCCGCAAACGCATTCAGCTGACGGCAATCGAAGAGACGCGGCCGCAGCCCGCACCC
TGCGACCTGCATTATCTCTTTGCGCCGCTCAAGGTCGGCCGCATGGATTATCTCGTGCAGAAGGCCGTGGAGATGGGCGC
CGGCCTGCTGCAGCCGGTCATGACCCAGCACGTCCAGGGCAAGATCACCAATCTCGACAAGCTGCGCGCCAATGTCGTCG
AGGCGGCGGAGCAGTGCGGCGTTCTCGGCATTCCCGATGTGGCCGAGCCGGTGCGGCTTCTCGACCTGCTCGACCGCTGG
CCGAAAGAGCGGCGCATCATCTATTGCGACGAGGGCGATGCCGGGCAAAACCCGCTGCCGGTGCTGTCGGCGATCCGGGA
AAGGCACCTCGCCTTGCTCGTCGGGCCGGAGGGCGGCTTTTCCGAGGAAGAGCGGGCGCGGCTTCGAAGCCTCGATTTCG
TCACCGCCATTCCGCTTGGACCGCGCATCCTGCGCGCCGATACGGCAGCCGTTGCGGCACTCGCCGTGGTGCAGGCAGCG
ATCGGCGACTGGAGCTGA

Upstream 100 bases:

>100_bases
ATCGCCGCCTGGATCGTCACGGTGCCGGTTTCGGCAGCGCTTGCGGCAGTGATTTATTGGGTTATGTTCGCGCTTTTCGT
CTGACACACGCGAGTCCTAA

Downstream 100 bases:

>100_bases
CGAGAAAAACGGGTGGACATGGAAATTGCTGTTATTTTTTTGATGCGTCATTGAAAGAATTTCACTTGCAACATACGTGA
CTTGGGTCCTAATCAGCCTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRANFRMQRLFVDAPLSSGAALEANADQFNYLANVLRMEEGGEVLLFNGRDGEWKATLTFPTRKRIQLTAIEETRPQPAP
CDLHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQGKITNLDKLRANVVEAAEQCGVLGIPDVAEPVRLLDLLDRW
PKERRIIYCDEGDAGQNPLPVLSAIRERHLALLVGPEGGFSEEERARLRSLDFVTAIPLGPRILRADTAAVAALAVVQAA
IGDWS

Sequences:

>Translated_245_residues
MRANFRMQRLFVDAPLSSGAALEANADQFNYLANVLRMEEGGEVLLFNGRDGEWKATLTFPTRKRIQLTAIEETRPQPAP
CDLHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQGKITNLDKLRANVVEAAEQCGVLGIPDVAEPVRLLDLLDRW
PKERRIIYCDEGDAGQNPLPVLSAIRERHLALLVGPEGGFSEEERARLRSLDFVTAIPLGPRILRADTAAVAALAVVQAA
IGDWS
>Mature_245_residues
MRANFRMQRLFVDAPLSSGAALEANADQFNYLANVLRMEEGGEVLLFNGRDGEWKATLTFPTRKRIQLTAIEETRPQPAP
CDLHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQGKITNLDKLRANVVEAAEQCGVLGIPDVAEPVRLLDLLDRW
PKERRIIYCDEGDAGQNPLPVLSAIRERHLALLVGPEGGFSEEERARLRSLDFVTAIPLGPRILRADTAAVAALAVVQAA
IGDWS

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=255, Percent_Identity=27.843137254902, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27035; Mature: 27035

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANFRMQRLFVDAPLSSGAALEANADQFNYLANVLRMEEGGEVLLFNGRDGEWKATLTF
CCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEC
PTRKRIQLTAIEETRPQPAPCDLHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQG
CCCCEEEEEEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
KITNLDKLRANVVEAAEQCGVLGIPDVAEPVRLLDLLDRWPKERRIIYCDEGDAGQNPLP
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
VLSAIRERHLALLVGPEGGFSEEERARLRSLDFVTAIPLGPRILRADTAAVAALAVVQAA
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
IGDWS
HCCCC
>Mature Secondary Structure
MRANFRMQRLFVDAPLSSGAALEANADQFNYLANVLRMEEGGEVLLFNGRDGEWKATLTF
CCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEC
PTRKRIQLTAIEETRPQPAPCDLHYLFAPLKVGRMDYLVQKAVEMGAGLLQPVMTQHVQG
CCCCEEEEEEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
KITNLDKLRANVVEAAEQCGVLGIPDVAEPVRLLDLLDRWPKERRIIYCDEGDAGQNPLP
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
VLSAIRERHLALLVGPEGGFSEEERARLRSLDFVTAIPLGPRILRADTAAVAALAVVQAA
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
IGDWS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]