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The map label for this gene is pitB [C]

Identifier: 209548050

GI number: 209548050

Start: 453598

End: 455097

Strand: Reverse

Name: pitB [C]

Synonym: Rleg2_0443

Alternate gene names: 209548050

Gene position: 455097-453598 (Counterclockwise)

Preceding gene: 209548051

Following gene: 209548049

Centisome position: 10.03

GC content: 65.27

Gene sequence:

>1500_bases
ATGCCGCCACGTCCCACAGTCCTCACGAAACGCACGCTCGACAAGGATCTCGACAAAATAACGCATGCCGAGGAGGCGGC
GAAGCATGTTTTGCGGCGGCTGGTGGCGCCTGGCCTCGGGCTGATCTTCGTCGGCCTGGTCATGCTGTTTGCCGGTGTCT
ATGTGCTCGACCAGCCGGGAGCGGTGCTCGTCGTGGCCGCGGCAGCGCTTGCCGGCTACATGGCGATGAACATCGGCGCC
AATGACGTGACCAACAATGTCGGTGCGGCCGTCGGGGCGCGCGCCATGACGATGGGCCAGGCGCTGATCATCGCCGCTAT
TTTCGAAGTTCTCGGCGCCACGGTCGCCGGCGGCGAGGTCGTCAAGACGATTTCCTCCAACATCGTCGATACCGTCCAGG
TTCCGCAGGCGCTGCTCGGCTGGATCATGATGGCGGCGCTGATGGCGGCCGCCCTCTGGATCAATCTCGCCACCTGGATG
AACGCGCCGGTCTCCACCACCCATGCGATCGTCGGCGCGGTGATCGGTGCCGGCATTGCGGCCGTCGGGACGGAGCCGGT
AAACTGGCGGGTGATGCTGGAGATCACCTCGAGCTGGATCACCTCGCCGCTGATCGGCGGGCTGATTGCCGCCGGGCTGC
TCTACCTCGTCAAGACGCTGATCATCTATCGCGACGACAAGATCGCCGCCGCCCGGCGCTGGGTGCCGGTGCTGGTCGCG
GTGATGGCCGGCGGCTTCATGGCCTATATGGTGCTGCAGCTGTCGCCCATCGGCAAATTTCCGCCGTTCACCGTCATCCT
CATCGGCATCGGTATCGGGCTGGTCAGCTGGCTTGCCGCCCGGCCGCTTGTGCTCGCCCAGGCGCACAATCTCGAAAACC
GCAACAGCTCGCTGCGCGTGCTGTTCCGGCTGCCGCTGATCGGCTCGGCGGCGCTGCTGTCCTTCGCGCATGGCGCAAAC
GATGTTTCGAACGCGGTCGGGCCGCTTTCGGCGATCGTCCATTCGGTCGGCATCGGCGGCGACGGCGGCGTGGGTCATCC
GCCGCTCTGGGTGATGCTGATCGGCGCCTTCGGCATCTCCGTCGGCCTGCTGCTGTTCGGGCCGCGCCTCATCCGCCTGG
TCGGCGAAGAGATCACCAAGCTCAATCCGATGCGGGCCTATTGCGTCGCGCTGTCGACCGCTTTCACCGTCATCGCCGCC
TCCTGGCTCGGCCTGCCGGTCAGCACCACCCACATTGCCATCGGCGCCGTCTTCGGCGTCGGCTTCTTCCGCGAATGGTA
CACCCGCCACTCGAAGCGCCGCATCGCCTATATCAGGCGCCGGGCCGAGAACTTCGATATCGACGAGCCGGAGGATCCGA
ACATCCACGAGACGCGGCGGCGTTATCTCGTGCGCCGCTCGCATTTCATGACGATCATCGCCGCCTGGATCGTCACGGTG
CCGGTTTCGGCAGCGCTTGCGGCAGTGATTTATTGGGTTATGTTCGCGCTTTTCGTCTGA

Upstream 100 bases:

>100_bases
GCAGGGATCTGCGGCAAAGCAAATTCCGGCGGAATGAATCCTGCCATCTTGCGCTGCTAGGAAAGTGGCGTAGAAGCAGA
TTTCATCCCCGGAATAAAGA

Downstream 100 bases:

>100_bases
CACACGCGAGTCCTAAATGCGCGCCAATTTCCGCATGCAACGGCTTTTCGTCGACGCGCCGCTTTCCAGCGGCGCCGCCC
TCGAGGCGAATGCCGACCAG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPPRPTVLTKRTLDKDLDKITHAEEAAKHVLRRLVAPGLGLIFVGLVMLFAGVYVLDQPGAVLVVAAAALAGYMAMNIGA
NDVTNNVGAAVGARAMTMGQALIIAAIFEVLGATVAGGEVVKTISSNIVDTVQVPQALLGWIMMAALMAAALWINLATWM
NAPVSTTHAIVGAVIGAGIAAVGTEPVNWRVMLEITSSWITSPLIGGLIAAGLLYLVKTLIIYRDDKIAAARRWVPVLVA
VMAGGFMAYMVLQLSPIGKFPPFTVILIGIGIGLVSWLAARPLVLAQAHNLENRNSSLRVLFRLPLIGSAALLSFAHGAN
DVSNAVGPLSAIVHSVGIGGDGGVGHPPLWVMLIGAFGISVGLLLFGPRLIRLVGEEITKLNPMRAYCVALSTAFTVIAA
SWLGLPVSTTHIAIGAVFGVGFFREWYTRHSKRRIAYIRRRAENFDIDEPEDPNIHETRRRYLVRRSHFMTIIAAWIVTV
PVSAALAAVIYWVMFALFV

Sequences:

>Translated_499_residues
MPPRPTVLTKRTLDKDLDKITHAEEAAKHVLRRLVAPGLGLIFVGLVMLFAGVYVLDQPGAVLVVAAAALAGYMAMNIGA
NDVTNNVGAAVGARAMTMGQALIIAAIFEVLGATVAGGEVVKTISSNIVDTVQVPQALLGWIMMAALMAAALWINLATWM
NAPVSTTHAIVGAVIGAGIAAVGTEPVNWRVMLEITSSWITSPLIGGLIAAGLLYLVKTLIIYRDDKIAAARRWVPVLVA
VMAGGFMAYMVLQLSPIGKFPPFTVILIGIGIGLVSWLAARPLVLAQAHNLENRNSSLRVLFRLPLIGSAALLSFAHGAN
DVSNAVGPLSAIVHSVGIGGDGGVGHPPLWVMLIGAFGISVGLLLFGPRLIRLVGEEITKLNPMRAYCVALSTAFTVIAA
SWLGLPVSTTHIAIGAVFGVGFFREWYTRHSKRRIAYIRRRAENFDIDEPEDPNIHETRRRYLVRRSHFMTIIAAWIVTV
PVSAALAAVIYWVMFALFV
>Mature_498_residues
PPRPTVLTKRTLDKDLDKITHAEEAAKHVLRRLVAPGLGLIFVGLVMLFAGVYVLDQPGAVLVVAAAALAGYMAMNIGAN
DVTNNVGAAVGARAMTMGQALIIAAIFEVLGATVAGGEVVKTISSNIVDTVQVPQALLGWIMMAALMAAALWINLATWMN
APVSTTHAIVGAVIGAGIAAVGTEPVNWRVMLEITSSWITSPLIGGLIAAGLLYLVKTLIIYRDDKIAAARRWVPVLVAV
MAGGFMAYMVLQLSPIGKFPPFTVILIGIGIGLVSWLAARPLVLAQAHNLENRNSSLRVLFRLPLIGSAALLSFAHGAND
VSNAVGPLSAIVHSVGIGGDGGVGHPPLWVMLIGAFGISVGLLLFGPRLIRLVGEEITKLNPMRAYCVALSTAFTVIAAS
WLGLPVSTTHIAIGAVFGVGFFREWYTRHSKRRIAYIRRRAENFDIDEPEDPNIHETRRRYLVRRSHFMTIIAAWIVTVP
VSAALAAVIYWVMFALFV

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=283, Percent_Identity=30.3886925795053, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI5803173, Length=181, Percent_Identity=34.8066298342541, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1789360, Length=154, Percent_Identity=33.1168831168831, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25146401, Length=507, Percent_Identity=28.4023668639053, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71981576, Length=476, Percent_Identity=30.4621848739496, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17557328, Length=466, Percent_Identity=26.6094420600858, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17539280, Length=191, Percent_Identity=35.0785340314136, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566716, Length=214, Percent_Identity=33.1775700934579, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17536725, Length=145, Percent_Identity=32.4137931034483, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=117, Percent_Identity=37.6068376068376, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356511, Length=203, Percent_Identity=35.9605911330049, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53233; Mature: 53102

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRPTVLTKRTLDKDLDKITHAEEAAKHVLRRLVAPGLGLIFVGLVMLFAGVYVLDQPG
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCC
AVLVVAAAALAGYMAMNIGANDVTNNVGAAVGARAMTMGQALIIAAIFEVLGATVAGGEV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VKTISSNIVDTVQVPQALLGWIMMAALMAAALWINLATWMNAPVSTTHAIVGAVIGAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
AVGTEPVNWRVMLEITSSWITSPLIGGLIAAGLLYLVKTLIIYRDDKIAAARRWVPVLVA
HCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VMAGGFMAYMVLQLSPIGKFPPFTVILIGIGIGLVSWLAARPLVLAQAHNLENRNSSLRV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEE
LFRLPLIGSAALLSFAHGANDVSNAVGPLSAIVHSVGIGGDGGVGHPPLWVMLIGAFGIS
EEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VGLLLFGPRLIRLVGEEITKLNPMRAYCVALSTAFTVIAASWLGLPVSTTHIAIGAVFGV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GFFREWYTRHSKRRIAYIRRRAENFDIDEPEDPNIHETRRRYLVRRSHFMTIIAAWIVTV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PVSAALAAVIYWVMFALFV
HHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PPRPTVLTKRTLDKDLDKITHAEEAAKHVLRRLVAPGLGLIFVGLVMLFAGVYVLDQPG
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCC
AVLVVAAAALAGYMAMNIGANDVTNNVGAAVGARAMTMGQALIIAAIFEVLGATVAGGEV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VKTISSNIVDTVQVPQALLGWIMMAALMAAALWINLATWMNAPVSTTHAIVGAVIGAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
AVGTEPVNWRVMLEITSSWITSPLIGGLIAAGLLYLVKTLIIYRDDKIAAARRWVPVLVA
HCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VMAGGFMAYMVLQLSPIGKFPPFTVILIGIGIGLVSWLAARPLVLAQAHNLENRNSSLRV
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEE
LFRLPLIGSAALLSFAHGANDVSNAVGPLSAIVHSVGIGGDGGVGHPPLWVMLIGAFGIS
EEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
VGLLLFGPRLIRLVGEEITKLNPMRAYCVALSTAFTVIAASWLGLPVSTTHIAIGAVFGV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GFFREWYTRHSKRRIAYIRRRAENFDIDEPEDPNIHETRRRYLVRRSHFMTIIAAWIVTV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PVSAALAAVIYWVMFALFV
HHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]