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The map label for this gene is 209548048

Identifier: 209548048

GI number: 209548048

Start: 451322

End: 452695

Strand: Reverse

Name: 209548048

Synonym: Rleg2_0441

Alternate gene names: NA

Gene position: 452695-451322 (Counterclockwise)

Preceding gene: 209548049

Following gene: 209548047

Centisome position: 9.98

GC content: 62.88

Gene sequence:

>1374_bases
ATGGCCCGCGACACTACCGACCAGACACCGCTCTCTTCGGTTCAGGATCTGACCGATTATATCGCCGCCGGCAACAAGCC
GCGGGAGCGCTTCCGGATCGGCACCGAACACGAGAAATTCGCCTTCTTCCGCGCCGACAACAGCCCGGTTCCCTATTTCG
GCGACGCCAGCATTTCCGCCCTTTTGACCGGCCTGCAGGCGAAAAGCGGCTGGGAGCCGATCATGGACGGCGGCAACATC
ATCGGCCTGGCCGAGCAGAGCGGCATGGGTGCGATCTCGATCGAGCCCGGCGGCCAGTTCGAACTGTCCGGCGCGCCGTT
GGAAACGATCCACGAAACCTGCAGGGAATCGAACCAGCATCTGGCGACGCTGCGCGAAATCGCCGAGCCGATGGGCATCC
GCTTCCTCGGCATCGGCGGCAGCCCAAAATGGACCTATGCGGAAACCCCGCAGATGCCGAAATCGCGCTACGAGATCATG
ACCCGCTATATGCCGAAGGTCGGCAGCCAGGGTCTCGACATGATGTACCGCACCTGTACGATCCAGGTGAATCTCGATTT
TTCCTCTGAAGACGACATGCGCAAGAAAATGCGCGTTTCGATGAAGCTGCAATCGCTGGCAACCGCCCTCTTTGCATCCT
CGCCCTTCACCGAGGGCAAGCCGAACGGGCTGCTCTCCTGGCGCGGCGATATCTGGCGCGACACCGACAACCGGCGCTCG
GGCCTGCTCGATTTCACCTTCCGCGACGATTTCGGCTTCCGCGACTATGCCGAATGGGCGCTCGACGTGCCGATGTATTT
CATCGTCCGCGACGGCCGCTATCACGACTGTACCCATGTCACTTTCCGTCAGTTCATGAATGGCGCATTGAAGGGTGAGA
TCGCCGATCCGGCGCCGACGATGGGCGACTGGACGAACCATCTTTCGACGCTCTTCCCCGACGTGCGGCTGAAGCGTTTC
CTCGAAATGCGCGGCGCCGACGGCGGCCCGTGGCGGCGCATCTGTGCCTTGCCGGCCTTCTGGGTCGGCCTGCTCTACGA
CGATGCCGCGCTCGATGCCGCCGACCAGTTGACAAAGGATTGGAGTTTTGCCGAGATCGGCGCGCTGCGCGATGCCGTTC
CGGCGCAGGGGCTGAAGGCCGACTTTCGCGGGCACCCGCTCTTCGACATGGCGCGCGAAGTGGTCGGCATTTCAAGGGCC
GGCCTCAAGGCGCGCGGCAAGCTCAACCGGGAAGGCCAGGACGAGACGATCTTCCTGGCGCCGCTCGACGAGGTGCTCGC
CAAGAAGGCGACGCTCGCCGAGGATCTGCTGTCGCTCTATCACGGCCGGTGGAACGGTTCCGTCGAACCGGTGTTCGAGG
ACTATCAATACTGA

Upstream 100 bases:

>100_bases
CATTGAAAGAATTTCACTTGCAACATACGTGACTTGGGTCCTAATCAGCCTTCCAGCTGCCCGGCCCCCTGGGCGCCTTT
TTTGAATACAGGTACCCCGC

Downstream 100 bases:

>100_bases
GCCTGCCCGGGTCTTCGCCAGCTCGGAAAACCACCCGGCAGTTTGCAGGTACGGCTTTAAGTTCTTATAGCAAAAAGCCG
TTTGCACATTTCCTTTTCCG

Product: glutamate/cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MARDTTDQTPLSSVQDLTDYIAAGNKPRERFRIGTEHEKFAFFRADNSPVPYFGDASISALLTGLQAKSGWEPIMDGGNI
IGLAEQSGMGAISIEPGGQFELSGAPLETIHETCRESNQHLATLREIAEPMGIRFLGIGGSPKWTYAETPQMPKSRYEIM
TRYMPKVGSQGLDMMYRTCTIQVNLDFSSEDDMRKKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
GLLDFTFRDDFGFRDYAEWALDVPMYFIVRDGRYHDCTHVTFRQFMNGALKGEIADPAPTMGDWTNHLSTLFPDVRLKRF
LEMRGADGGPWRRICALPAFWVGLLYDDAALDAADQLTKDWSFAEIGALRDAVPAQGLKADFRGHPLFDMAREVVGISRA
GLKARGKLNREGQDETIFLAPLDEVLAKKATLAEDLLSLYHGRWNGSVEPVFEDYQY

Sequences:

>Translated_457_residues
MARDTTDQTPLSSVQDLTDYIAAGNKPRERFRIGTEHEKFAFFRADNSPVPYFGDASISALLTGLQAKSGWEPIMDGGNI
IGLAEQSGMGAISIEPGGQFELSGAPLETIHETCRESNQHLATLREIAEPMGIRFLGIGGSPKWTYAETPQMPKSRYEIM
TRYMPKVGSQGLDMMYRTCTIQVNLDFSSEDDMRKKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
GLLDFTFRDDFGFRDYAEWALDVPMYFIVRDGRYHDCTHVTFRQFMNGALKGEIADPAPTMGDWTNHLSTLFPDVRLKRF
LEMRGADGGPWRRICALPAFWVGLLYDDAALDAADQLTKDWSFAEIGALRDAVPAQGLKADFRGHPLFDMAREVVGISRA
GLKARGKLNREGQDETIFLAPLDEVLAKKATLAEDLLSLYHGRWNGSVEPVFEDYQY
>Mature_456_residues
ARDTTDQTPLSSVQDLTDYIAAGNKPRERFRIGTEHEKFAFFRADNSPVPYFGDASISALLTGLQAKSGWEPIMDGGNII
GLAEQSGMGAISIEPGGQFELSGAPLETIHETCRESNQHLATLREIAEPMGIRFLGIGGSPKWTYAETPQMPKSRYEIMT
RYMPKVGSQGLDMMYRTCTIQVNLDFSSEDDMRKKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRSG
LLDFTFRDDFGFRDYAEWALDVPMYFIVRDGRYHDCTHVTFRQFMNGALKGEIADPAPTMGDWTNHLSTLFPDVRLKRFL
EMRGADGGPWRRICALPAFWVGLLYDDAALDAADQLTKDWSFAEIGALRDAVPAQGLKADFRGHPLFDMAREVVGISRAG
LKARGKLNREGQDETIFLAPLDEVLAKKATLAEDLLSLYHGRWNGSVEPVFEDYQY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51266; Mature: 51134

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDTTDQTPLSSVQDLTDYIAAGNKPRERFRIGTEHEKFAFFRADNSPVPYFGDASISA
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHH
LLTGLQAKSGWEPIMDGGNIIGLAEQSGMGAISIEPGGQFELSGAPLETIHETCRESNQH
HHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCHHH
LATLREIAEPMGIRFLGIGGSPKWTYAETPQMPKSRYEIMTRYMPKVGSQGLDMMYRTCT
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEEEE
IQVNLDFSSEDDMRKKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
GLLDFTFRDDFGFRDYAEWALDVPMYFIVRDGRYHDCTHVTFRQFMNGALKGEIADPAPT
CEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
MGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYDDAALDAADQLTKD
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WSFAEIGALRDAVPAQGLKADFRGHPLFDMAREVVGISRAGLKARGKLNREGQDETIFLA
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
PLDEVLAKKATLAEDLLSLYHGRWNGSVEPVFEDYQY
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
>Mature Secondary Structure 
ARDTTDQTPLSSVQDLTDYIAAGNKPRERFRIGTEHEKFAFFRADNSPVPYFGDASISA
CCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHH
LLTGLQAKSGWEPIMDGGNIIGLAEQSGMGAISIEPGGQFELSGAPLETIHETCRESNQH
HHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCHHH
LATLREIAEPMGIRFLGIGGSPKWTYAETPQMPKSRYEIMTRYMPKVGSQGLDMMYRTCT
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEEEE
IQVNLDFSSEDDMRKKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDNRRS
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
GLLDFTFRDDFGFRDYAEWALDVPMYFIVRDGRYHDCTHVTFRQFMNGALKGEIADPAPT
CEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
MGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYDDAALDAADQLTKD
CCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WSFAEIGALRDAVPAQGLKADFRGHPLFDMAREVVGISRAGLKARGKLNREGQDETIFLA
CCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
PLDEVLAKKATLAEDLLSLYHGRWNGSVEPVFEDYQY
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA