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The map label for this gene is dppA [H]

Identifier: 209547998

GI number: 209547998

Start: 403328

End: 404923

Strand: Direct

Name: dppA [H]

Synonym: Rleg2_0390

Alternate gene names: 209547998

Gene position: 403328-404923 (Clockwise)

Preceding gene: 209547997

Following gene: 209547999

Centisome position: 8.89

GC content: 60.34

Gene sequence:

>1596_bases
ATGAAAAAGATCTCTGTCATGCTGGCAGCGACGGCCCTGATTTCGGTCATGGCGACGTCGGCCTGGTCCAAGACCCTTGT
TTATTGCTCCGAGGGTTCGCCTGAGGGTTTCGACCCGAGCCTCTATACGGCTGGCACGACCTTCGACGCCTCGTCGCGTA
CGGTCTATAGCCGCCTGGTCGAATTCAAGCATGGCGGTACCGAGATCGAACCGGGCCTCGCCGACAGCTGGAGCGTTTCG
GCCGACGGCACGGAATACACCTTCAAGCTTCATCCCGGCGTCAAGTATCAGACCACCGACTTCTTCACGCCGACGCGCGA
TTTCAACGCCGACGACGTCGTGTTCTCCTTCGAGCGCCAGCTGAAGGCCGACAATCCGTGGAACAAGTATGTCGAGGGCG
GTTCTTATGAATACGCCGCCGGCATGGGCTTCCCGGATCTGATCAAGTCGATCGAAAAGGTCGACGACCTCACGGTCAAG
TTCACGCTCAACCATCCCGAAGCGCCGTTCCTTGCCGATCTGGCGATGGACTTCGCCTCGATCGTTTCCAAGGAATATGC
CGACAAGCTCGCCGCCGACGGCAAGATGGCGCAGCTCAACCAGCAGCCCCTCGGCACCGGCCCCTTCACCTTCGTCGCCT
ACCAGCCGGATGCCGTCATCCGCTACAAGGCCAACGAAACCTATTTCAAGGGCAAGGAAAAGATTGACGATCTGGTTTTC
GCCATCACCTCTGACGCCGCCGTCCGCGCGCAGAAGCTGAAGGCCGGCGAATGCCACCTGATCCCCTATCCGAATGCGGC
TGACGTTCCCGAGTTGAAGAAGGACGGCAATCTGACGGTGATGGAACAGGCCGGCCTGAATGTCGGCTTCCTCGCCTACA
ACACGCAGATGGCCCCGTTCGACAAGCCGGAAGTTCGCCGTGCGCTCAACATGGCGATCAACAAGCAGGCGATCATCGAC
GCCGTCTTCCAGGGCGCAGCGGCTGTTGCCAAGAACCCGATCCCGCCGACGATGTGGTCCTATAACGACGCCGTTCAGGA
CGACAAGTACGATCCGGATGCCGCCAAGAAGGCTCTCGCCGATGCCGGCGTCAAGGATCTCAGCATGAAGGTCTGGGCGA
TGCCGGTGTCGCGTCCCTACATGCTGAACGCGCGCCGCGCCGCCGAACTGATCCAGGCCGATTTCGCCAAGGCCGGCGTC
AAGGTCGAGATCGTTACCCATGAATGGGCCGAATATCTGAAGCTCTCCTCCGACGTGAAGCGCGACGGCGCCGTCATCCT
CGGCTGGACCGGCGACAACGGCGACCCGGATAACTTCATGGATACGCTGCTTGGCTGCGATGCCGTCGGCGGCAACAACC
GTGCTCAGTGGTGCAACAAGGAATATGACGACCTGATGACCAAGGCCAAGCTGACCGCCGATGTCGGCGAGCGCACCAAG
GCCTATGAGCAGGCACAGCTGATCTTCAAGAAGGAAGCCCCCTGGGCGACCCTCGATCACTCGCTCGTCTTCGTTCCGAT
GAGCAAGAAGGTCTCCGGCTTCTTCATGGATCCGCTCGGCATTCACCGCTTCGACGGCGTCGACGTATCCGAATAA

Upstream 100 bases:

>100_bases
CCGTTGCTTTCAGTAGATAAATGTCGTTCCATCGACCGTCGATCGGGATGCATATCTGCGGTCTCCAGACATCAAAAAAG
AACAACCGGGAAACAATATT

Downstream 100 bases:

>100_bases
CAAGAAAAATGTAAGAATGCCCGGCAGAGCTGGGCGCAGCCGGCGGTCAGGCACCCCCTGACCGCCGGAAACTATTGGAC
ATCTGCCATGTTGCGTTTTT

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MKKISVMLAATALISVMATSAWSKTLVYCSEGSPEGFDPSLYTAGTTFDASSRTVYSRLVEFKHGGTEIEPGLADSWSVS
ADGTEYTFKLHPGVKYQTTDFFTPTRDFNADDVVFSFERQLKADNPWNKYVEGGSYEYAAGMGFPDLIKSIEKVDDLTVK
FTLNHPEAPFLADLAMDFASIVSKEYADKLAADGKMAQLNQQPLGTGPFTFVAYQPDAVIRYKANETYFKGKEKIDDLVF
AITSDAAVRAQKLKAGECHLIPYPNAADVPELKKDGNLTVMEQAGLNVGFLAYNTQMAPFDKPEVRRALNMAINKQAIID
AVFQGAAAVAKNPIPPTMWSYNDAVQDDKYDPDAAKKALADAGVKDLSMKVWAMPVSRPYMLNARRAAELIQADFAKAGV
KVEIVTHEWAEYLKLSSDVKRDGAVILGWTGDNGDPDNFMDTLLGCDAVGGNNRAQWCNKEYDDLMTKAKLTADVGERTK
AYEQAQLIFKKEAPWATLDHSLVFVPMSKKVSGFFMDPLGIHRFDGVDVSE

Sequences:

>Translated_531_residues
MKKISVMLAATALISVMATSAWSKTLVYCSEGSPEGFDPSLYTAGTTFDASSRTVYSRLVEFKHGGTEIEPGLADSWSVS
ADGTEYTFKLHPGVKYQTTDFFTPTRDFNADDVVFSFERQLKADNPWNKYVEGGSYEYAAGMGFPDLIKSIEKVDDLTVK
FTLNHPEAPFLADLAMDFASIVSKEYADKLAADGKMAQLNQQPLGTGPFTFVAYQPDAVIRYKANETYFKGKEKIDDLVF
AITSDAAVRAQKLKAGECHLIPYPNAADVPELKKDGNLTVMEQAGLNVGFLAYNTQMAPFDKPEVRRALNMAINKQAIID
AVFQGAAAVAKNPIPPTMWSYNDAVQDDKYDPDAAKKALADAGVKDLSMKVWAMPVSRPYMLNARRAAELIQADFAKAGV
KVEIVTHEWAEYLKLSSDVKRDGAVILGWTGDNGDPDNFMDTLLGCDAVGGNNRAQWCNKEYDDLMTKAKLTADVGERTK
AYEQAQLIFKKEAPWATLDHSLVFVPMSKKVSGFFMDPLGIHRFDGVDVSE
>Mature_531_residues
MKKISVMLAATALISVMATSAWSKTLVYCSEGSPEGFDPSLYTAGTTFDASSRTVYSRLVEFKHGGTEIEPGLADSWSVS
ADGTEYTFKLHPGVKYQTTDFFTPTRDFNADDVVFSFERQLKADNPWNKYVEGGSYEYAAGMGFPDLIKSIEKVDDLTVK
FTLNHPEAPFLADLAMDFASIVSKEYADKLAADGKMAQLNQQPLGTGPFTFVAYQPDAVIRYKANETYFKGKEKIDDLVF
AITSDAAVRAQKLKAGECHLIPYPNAADVPELKKDGNLTVMEQAGLNVGFLAYNTQMAPFDKPEVRRALNMAINKQAIID
AVFQGAAAVAKNPIPPTMWSYNDAVQDDKYDPDAAKKALADAGVKDLSMKVWAMPVSRPYMLNARRAAELIQADFAKAGV
KVEIVTHEWAEYLKLSSDVKRDGAVILGWTGDNGDPDNFMDTLLGCDAVGGNNRAQWCNKEYDDLMTKAKLTADVGERTK
AYEQAQLIFKKEAPWATLDHSLVFVPMSKKVSGFFMDPLGIHRFDGVDVSE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=532, Percent_Identity=54.8872180451128, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1787551, Length=507, Percent_Identity=35.5029585798817, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1787762, Length=551, Percent_Identity=28.8566243194192, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1787052, Length=538, Percent_Identity=27.5092936802974, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789887, Length=431, Percent_Identity=25.9860788863109, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789397, Length=465, Percent_Identity=24.5161290322581, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58456; Mature: 58456

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKISVMLAATALISVMATSAWSKTLVYCSEGSPEGFDPSLYTAGTTFDASSRTVYSRLV
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHH
EFKHGGTEIEPGLADSWSVSADGTEYTFKLHPGVKYQTTDFFTPTRDFNADDVVFSFERQ
HHCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEHHH
LKADNPWNKYVEGGSYEYAAGMGFPDLIKSIEKVDDLTVKFTLNHPEAPFLADLAMDFAS
HCCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHH
IVSKEYADKLAADGKMAQLNQQPLGTGPFTFVAYQPDAVIRYKANETYFKGKEKIDDLVF
HHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCEECCHHHHHHEEE
AITSDAAVRAQKLKAGECHLIPYPNAADVPELKKDGNLTVMEQAGLNVGFLAYNTQMAPF
EEECCHHHHHHHCCCCCEEEEECCCCCCCCCHHCCCCEEEEECCCCEEEEEEEECCCCCC
DKPEVRRALNMAINKQAIIDAVFQGAAAVAKNPIPPTMWSYNDAVQDDKYDPDAAKKALA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DAGVKDLSMKVWAMPVSRPYMLNARRAAELIQADFAKAGVKVEIVTHEWAEYLKLSSDVK
HCCHHHCEEEEEEEECCCCEEECHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCC
RDGAVILGWTGDNGDPDNFMDTLLGCDAVGGNNRAQWCNKEYDDLMTKAKLTADVGERTK
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
AYEQAQLIFKKEAPWATLDHSLVFVPMSKKVSGFFMDPLGIHRFDGVDVSE
HHHHHHHHEECCCCCEEECCCEEEEECCCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKISVMLAATALISVMATSAWSKTLVYCSEGSPEGFDPSLYTAGTTFDASSRTVYSRLV
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHH
EFKHGGTEIEPGLADSWSVSADGTEYTFKLHPGVKYQTTDFFTPTRDFNADDVVFSFERQ
HHCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEHHH
LKADNPWNKYVEGGSYEYAAGMGFPDLIKSIEKVDDLTVKFTLNHPEAPFLADLAMDFAS
HCCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHH
IVSKEYADKLAADGKMAQLNQQPLGTGPFTFVAYQPDAVIRYKANETYFKGKEKIDDLVF
HHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCEECCHHHHHHEEE
AITSDAAVRAQKLKAGECHLIPYPNAADVPELKKDGNLTVMEQAGLNVGFLAYNTQMAPF
EEECCHHHHHHHCCCCCEEEEECCCCCCCCCHHCCCCEEEEECCCCEEEEEEEECCCCCC
DKPEVRRALNMAINKQAIIDAVFQGAAAVAKNPIPPTMWSYNDAVQDDKYDPDAAKKALA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DAGVKDLSMKVWAMPVSRPYMLNARRAAELIQADFAKAGVKVEIVTHEWAEYLKLSSDVK
HCCHHHCEEEEEEEECCCCEEECHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCC
RDGAVILGWTGDNGDPDNFMDTLLGCDAVGGNNRAQWCNKEYDDLMTKAKLTADVGERTK
CCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
AYEQAQLIFKKEAPWATLDHSLVFVPMSKKVSGFFMDPLGIHRFDGVDVSE
HHHHHHHHEECCCCCEEECCCEEEEECCCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]