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The map label for this gene is otsA [H]

Identifier: 209547747

GI number: 209547747

Start: 128036

End: 129451

Strand: Reverse

Name: otsA [H]

Synonym: Rleg2_0136

Alternate gene names: 209547747

Gene position: 129451-128036 (Counterclockwise)

Preceding gene: 209547748

Following gene: 209547743

Centisome position: 2.85

GC content: 61.94

Gene sequence:

>1416_bases
ATGAGCCGTCTGATCGTCGTTTCCAATCGTGTTCCCGTGCCGAGCAAGGATGGGGGTGCTGCTGCCGGCGGCCTGGCCGT
TGCGCTGCAGGCAGCCCTGCAGGAGCGCGGCGGCATCTGGATGGGGTGGTCGGGAGAGTCGAGCGGCGATCGGGAACCGG
GCCCGCTGTCGCAACAGCAGAAGGGCAACATCACCTATGCGCTGACCGACCTCACCGACACCGACGTCGAGGAATATTAC
CGCGGTTTTGCCAATCGCGTTCTCTGGCCGATCTGCCACTATCGCCTGGACCTTGCCGAATATGGCCGGAAGGAAATGGC
CGGCTATTTCCGCGTCAACCGCTTCTTCGCGCACCGGCTGGCGCCGCTGATCGAGCCGGATGATATTATCTGGGTTCACG
ATTACCACCTCATTCCGCTGGCGGCGGAGCTGCGGCAGATGGGGCTGAAGAACCGGATCGGCTTCTTTCTGCATATCCCC
TGGCCGCCGGCCGACATCCTCGTCACCATGCCGGTCCACGAAGAAATCATGCGTGGGCTTTCGCATTACGATCTCGTCGG
CTTCCAGACCGACTACGACCTGCAGAACTTCGCCGGCTATCTCAGAAGGGAAGGCATTGGCGACGACCTCGGCAATGGTT
TGTTCGATTCGCACGGGCGGGTCTTCAAGGCCGGCGCCTATCCGATCGGCATCGAAACCGCTGGATTCGCAGCATTCGCC
GAAAAAGCCGCAAACAACGTCATGGTCCAGAAGACCCGGCGCAGCATTGAAGGCCGCGATATGATCATCGGGGTCGACCG
GCTCGATTATTCGAAGGGCATCATTGAGCGGCTGGAGGCGTTCGAGCGCTTCATCACCAGCAATCCGGCCTACCAGAACA
AGGTGACCTTTCTGCAGGTCACGCCGAAATCGCGATCGGAAGTTCCCGAATACGAGCAGATGCAGAAGATGGTCGCCGAA
CAGGCAGGCCGCGTGAACGGCGCCATCGGCACGGTCGACTGGGTGCCGATCCGCTATGTCAACCGATCGATCAGCCGCAA
CGTGCTCGCCGGCCTTTTCCGGCTGGCGACGATCGGGCTGGTCACGCCGCTGCGCGACGGCATGAACCTCGTCGCCAAGG
AATATGTCGCCGCCCAGGATCCGGATCGCCCCGGCGTATTGGTGCTATCCCGCTTCGCCGGCGCGGCCCGCGAGTTGAAG
GGTGCGCTGCTGGTCAATCCTTATGACGTCGAAGGCACGGCCAATGCCGTCGCCAAGGGCCTTGCCATGTCGCTTGCCGA
ACGCCGCGACCGCTGGAGGATGATGATGGACCATCTGCTCTCGCACGACGTCTCGCTCTGGTGCAAAAATTTCTTGGGGG
ATCTTGTGGCTGGTCCCGAGCTTCGGCCGGAGCATAGCTCCCGGACAGTCTCCTGA

Upstream 100 bases:

>100_bases
GCCTATCGAGCCGGGGACGACGCTCCGTTCCATCACTCCCATCGGCCGATGAGCAGGCGCTTCCATGAAAAGCTGAACCG
CTCTCAGGCAAAGGAACCCC

Downstream 100 bases:

>100_bases
TCGATCGGCATCCATCGTCATCGCATGAGGCGTCGAAGTTGCGCGGGCGGGCCATGTTTCCTGGACGCAAGGGCTGGCTG
CGGCGCTGGCGAAGTAAGCG

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSRLIVVSNRVPVPSKDGGAAAGGLAVALQAALQERGGIWMGWSGESSGDREPGPLSQQQKGNITYALTDLTDTDVEEYY
RGFANRVLWPICHYRLDLAEYGRKEMAGYFRVNRFFAHRLAPLIEPDDIIWVHDYHLIPLAAELRQMGLKNRIGFFLHIP
WPPADILVTMPVHEEIMRGLSHYDLVGFQTDYDLQNFAGYLRREGIGDDLGNGLFDSHGRVFKAGAYPIGIETAGFAAFA
EKAANNVMVQKTRRSIEGRDMIIGVDRLDYSKGIIERLEAFERFITSNPAYQNKVTFLQVTPKSRSEVPEYEQMQKMVAE
QAGRVNGAIGTVDWVPIRYVNRSISRNVLAGLFRLATIGLVTPLRDGMNLVAKEYVAAQDPDRPGVLVLSRFAGAARELK
GALLVNPYDVEGTANAVAKGLAMSLAERRDRWRMMMDHLLSHDVSLWCKNFLGDLVAGPELRPEHSSRTVS

Sequences:

>Translated_471_residues
MSRLIVVSNRVPVPSKDGGAAAGGLAVALQAALQERGGIWMGWSGESSGDREPGPLSQQQKGNITYALTDLTDTDVEEYY
RGFANRVLWPICHYRLDLAEYGRKEMAGYFRVNRFFAHRLAPLIEPDDIIWVHDYHLIPLAAELRQMGLKNRIGFFLHIP
WPPADILVTMPVHEEIMRGLSHYDLVGFQTDYDLQNFAGYLRREGIGDDLGNGLFDSHGRVFKAGAYPIGIETAGFAAFA
EKAANNVMVQKTRRSIEGRDMIIGVDRLDYSKGIIERLEAFERFITSNPAYQNKVTFLQVTPKSRSEVPEYEQMQKMVAE
QAGRVNGAIGTVDWVPIRYVNRSISRNVLAGLFRLATIGLVTPLRDGMNLVAKEYVAAQDPDRPGVLVLSRFAGAARELK
GALLVNPYDVEGTANAVAKGLAMSLAERRDRWRMMMDHLLSHDVSLWCKNFLGDLVAGPELRPEHSSRTVS
>Mature_470_residues
SRLIVVSNRVPVPSKDGGAAAGGLAVALQAALQERGGIWMGWSGESSGDREPGPLSQQQKGNITYALTDLTDTDVEEYYR
GFANRVLWPICHYRLDLAEYGRKEMAGYFRVNRFFAHRLAPLIEPDDIIWVHDYHLIPLAAELRQMGLKNRIGFFLHIPW
PPADILVTMPVHEEIMRGLSHYDLVGFQTDYDLQNFAGYLRREGIGDDLGNGLFDSHGRVFKAGAYPIGIETAGFAAFAE
KAANNVMVQKTRRSIEGRDMIIGVDRLDYSKGIIERLEAFERFITSNPAYQNKVTFLQVTPKSRSEVPEYEQMQKMVAEQ
AGRVNGAIGTVDWVPIRYVNRSISRNVLAGLFRLATIGLVTPLRDGMNLVAKEYVAAQDPDRPGVLVLSRFAGAARELKG
ALLVNPYDVEGTANAVAKGLAMSLAERRDRWRMMMDHLLSHDVSLWCKNFLGDLVAGPELRPEHSSRTVS

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=455, Percent_Identity=46.1538461538462, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI115533560, Length=413, Percent_Identity=30.5084745762712, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI115533558, Length=413, Percent_Identity=30.5084745762712, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71987012, Length=445, Percent_Identity=26.2921348314607, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319602, Length=466, Percent_Identity=33.4763948497854, Blast_Score=296, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6323537, Length=465, Percent_Identity=27.5268817204301, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323917, Length=444, Percent_Identity=26.3513513513513, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320279, Length=398, Percent_Identity=28.1407035175879, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19920676, Length=483, Percent_Identity=37.2670807453416, Blast_Score=294, Evalue=9e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52551; Mature: 52420

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIVVSNRVPVPSKDGGAAAGGLAVALQAALQERGGIWMGWSGESSGDREPGPLSQQQ
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCC
KGNITYALTDLTDTDVEEYYRGFANRVLWPICHYRLDLAEYGRKEMAGYFRVNRFFAHRL
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
APLIEPDDIIWVHDYHLIPLAAELRQMGLKNRIGFFLHIPWPPADILVTMPVHEEIMRGL
CCCCCCCCEEEEECCEEHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCHHHHHHCH
SHYDLVGFQTDYDLQNFAGYLRREGIGDDLGNGLFDSHGRVFKAGAYPIGIETAGFAAFA
HHHEEECCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCEEECCCCEECCCCCHHHHHH
EKAANNVMVQKTRRSIEGRDMIIGVDRLDYSKGIIERLEAFERFITSNPAYQNKVTFLQV
HHHHCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
TPKSRSEVPEYEQMQKMVAEQAGRVNGAIGTVDWVPIRYVNRSISRNVLAGLFRLATIGL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VTPLRDGMNLVAKEYVAAQDPDRPGVLVLSRFAGAARELKGALLVNPYDVEGTANAVAKG
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHH
LAMSLAERRDRWRMMMDHLLSHDVSLWCKNFLGDLVAGPELRPEHSSRTVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRLIVVSNRVPVPSKDGGAAAGGLAVALQAALQERGGIWMGWSGESSGDREPGPLSQQQ
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCC
KGNITYALTDLTDTDVEEYYRGFANRVLWPICHYRLDLAEYGRKEMAGYFRVNRFFAHRL
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
APLIEPDDIIWVHDYHLIPLAAELRQMGLKNRIGFFLHIPWPPADILVTMPVHEEIMRGL
CCCCCCCCEEEEECCEEHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEECCCHHHHHHCH
SHYDLVGFQTDYDLQNFAGYLRREGIGDDLGNGLFDSHGRVFKAGAYPIGIETAGFAAFA
HHHEEECCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCEEECCCCEECCCCCHHHHHH
EKAANNVMVQKTRRSIEGRDMIIGVDRLDYSKGIIERLEAFERFITSNPAYQNKVTFLQV
HHHHCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
TPKSRSEVPEYEQMQKMVAEQAGRVNGAIGTVDWVPIRYVNRSISRNVLAGLFRLATIGL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VTPLRDGMNLVAKEYVAAQDPDRPGVLVLSRFAGAARELKGALLVNPYDVEGTANAVAKG
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHH
LAMSLAERRDRWRMMMDHLLSHDVSLWCKNFLGDLVAGPELRPEHSSRTVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]