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The map label for this gene is ttdB

Identifier: 209400059

GI number: 209400059

Start: 4049984

End: 4050589

Strand: Direct

Name: ttdB

Synonym: ECH74115_4374

Alternate gene names: 209400059

Gene position: 4049984-4050589 (Clockwise)

Preceding gene: 209396443

Following gene: 209400804

Centisome position: 72.68

GC content: 53.47

Gene sequence:

>606_bases
ATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCACGTCGGCGATGTGATCTATCTGACCGG
TACGCTGGTGACCTGCCGTGACGTTTGTCACCGCCGATTGATCGAACTGAAACGTCCGCTCCCTTACGATCTCAACGGCA
AAGCGATTTTCCACGCTGGTCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGTCCGACAACCAGT
ATGCGTATGGAAAGTTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGTGGTCGGCAAAGGCGGAATGGGGCC
GCTGACCGAAGAAGGCTGTCAGAAATTCAAGGCGCTACATGTAATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCC
AGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAACTCGGGATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTC
GGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGA
TCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAA

Upstream 100 bases:

>100_bases
GCGTTGCTGTTTCTACCGGTTGCTGGGCGCATCGTCGCGGCACACTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTG
TCTCACACCCGGAGCGCGTT

Downstream 100 bases:

>100_bases
CCCTCCCGGAGAGGCTCGCCCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATACCT
TGCGCCGCTGGCGGTCATCG

Product: L(+)-tartrate dehydratase subunit beta

Products: NA

Alternate protein names: L-TTD beta

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELKRPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTS
MRMESFEREFIEQTGVKLVVGKGGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK

Sequences:

>Translated_201_residues
MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELKRPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTS
MRMESFEREFIEQTGVKLVVGKGGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK
>Mature_201_residues
MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELKRPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTS
MRMESFEREFIEQTGVKLVVGKGGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK

Specific function: Unknown

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family

Homologues:

Organism=Escherichia coli, GI1789443, Length=201, Percent_Identity=99.0049751243781, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787897, Length=181, Percent_Identity=29.2817679558011, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790564, Length=181, Percent_Identity=30.3867403314917, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTDB_ECO57 (Q8XBK5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91122
- PIR:   H85966
- RefSeq:   NP_289637.1
- RefSeq:   NP_311972.1
- ProteinModelPortal:   Q8XBK5
- SMR:   Q8XBK5
- EnsemblBacteria:   EBESCT00000023866
- EnsemblBacteria:   EBESCT00000055559
- GeneID:   916229
- GeneID:   958372
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4415
- KEGG:   ecs:ECs3945
- GeneTree:   EBGT00050000010386
- HOGENOM:   HBG655206
- OMA:   VIYHAGP
- ProtClustDB:   PRK08228
- BioCyc:   ECOL83334:ECS3945-MONOMER
- InterPro:   IPR004647
- Gene3D:   G3DSA:3.20.130.10
- TIGRFAMs:   TIGR00723

Pfam domain/function: PF05683 Fumerase_C

EC number: =4.2.1.32

Molecular weight: Translated: 22661; Mature: 22661

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 37-37

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELKRPLPYDLNGKAIFHAG
CCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
PIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGKGGMGPLTEEGCQKFKALH
CEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHE
VIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEFGPLIVSIDTHGNNLIAENKK
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCEEHHHHH
LFAERRDPIVEEICEHVHYIK
HHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELKRPLPYDLNGKAIFHAG
CCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
PIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGKGGMGPLTEEGCQKFKALH
CEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHE
VIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEFGPLIVSIDTHGNNLIAENKK
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCEEHHHHH
LFAERRDPIVEEICEHVHYIK
HHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796