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The map label for this gene is ddtA

Identifier: 209396443

GI number: 209396443

Start: 4049076

End: 4049987

Strand: Direct

Name: ddtA

Synonym: ECH74115_4373

Alternate gene names: 209396443

Gene position: 4049076-4049987 (Clockwise)

Preceding gene: 209397618

Following gene: 209400059

Centisome position: 72.67

GC content: 54.06

Gene sequence:

>912_bases
ATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACGGAGATTGTCGCTAACTTTACCGCCATGATTTCTAC
CCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACC
ATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTC
GTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCGACCATCAA
AGCGCCGCTGCGTCACAATGCGGTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGG
TCACCTGGGATATCGTCCCCGACGGTGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCAGGC
CGCTCGAAAGTGTTAATGCCGTCAGAAGGCTACGAAGGCGTGGTGAAATTCGTCTTCGAAAATATCTCCACCCTCGCAGT
AAACGCCTGTCCACCGGTACTGGTGGGCGTGGGCATCGCCACCTCGGTGGAAACCGCCGCCGTACTCTCGCGTAAAGCCA
TTTTGCGCCCGATTGGCAGCCGCCACCCCAATCCAAAAGCGGCAGAGCTGGAGCTACGCCTGGAAGAAGGACTCAACCGT
CTGGGGATTGGTCCACAAGGGCTAACTGGCAACAGTTCAGTGATGGGCGTGCATATCGAATCTGCCGCCCGCCATCCGTC
AACCATCGGCGTTGCTGTTTCTACCGGTTGCTGGGCGCATCGTCGCGGCACACTGCTGGTTCATGCCGATCTCACCTTTG
AAAATCTGTCTCACACCCGGAGCGCGTTATGA

Upstream 100 bases:

>100_bases
TTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAA
ATACAAAAACTGGAGTTGCC

Downstream 100 bases:

>100_bases
AAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCACGTCGGCGATGTGATCTATCTGACCGGTACG
CTGGTGACCTGCCGTGACGT

Product: tartrate dehydratase subunit alpha

Products: NA

Alternate protein names: L-TTD alpha

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFF
VKVGSRFPLLGELQSILKQAVEEATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPIGSRHPNPKAAELELRLEEGLNR
LGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTGCWAHRRGTLLVHADLTFENLSHTRSAL

Sequences:

>Translated_303_residues
MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFF
VKVGSRFPLLGELQSILKQAVEEATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPIGSRHPNPKAAELELRLEEGLNR
LGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTGCWAHRRGTLLVHADLTFENLSHTRSAL
>Mature_303_residues
MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFF
VKVGSRFPLLGELQSILKQAVEEATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPIGSRHPNPKAAELELRLEEGLNR
LGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTGCWAHRRGTLLVHADLTFENLSHTRSAL

Specific function: Unknown

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family

Homologues:

Organism=Escherichia coli, GI1789442, Length=303, Percent_Identity=99.009900990099, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI1790564, Length=258, Percent_Identity=27.906976744186, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787897, Length=258, Percent_Identity=27.5193798449612, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTDA_ECO57 (Q8XBK7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G85966
- PIR:   H91121
- RefSeq:   NP_289636.1
- RefSeq:   NP_311971.1
- EnsemblBacteria:   EBESCT00000028526
- EnsemblBacteria:   EBESCT00000059282
- GeneID:   916192
- GeneID:   958530
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4414
- KEGG:   ecs:ECs3944
- GeneTree:   EBGT00050000010386
- HOGENOM:   HBG548096
- OMA:   INACPPL
- ProtClustDB:   PRK08230
- BioCyc:   ECOL83334:ECS3944-MONOMER
- InterPro:   IPR004646
- TIGRFAMs:   TIGR00722

Pfam domain/function: PF05681 Fumerase

EC number: =4.2.1.32

Molecular weight: Translated: 32677; Mature: 32677

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVEEATIKAPLRHNAVEIFDE
HHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHH
VNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPGRSKVLMPSEGYEGVVKFVFE
HCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHH
NISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPIGSRHPNPKAAELELRLEEGLNR
HHHHEEECCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEHHHCHHH
LGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTGCWAHRRGTLLVHADLTFENLSHTR
HCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCEEECCCCEEEEECCCHHHHHHHH
SAL
HCC
>Mature Secondary Structure
MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVEEATIKAPLRHNAVEIFDE
HHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHH
VNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPGRSKVLMPSEGYEGVVKFVFE
HCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHH
NISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPIGSRHPNPKAAELELRLEEGLNR
HHHHEEECCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEHHHCHHH
LGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTGCWAHRRGTLLVHADLTFENLSHTR
HCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCEEECCCCEEEEECCCHHHHHHHH
SAL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796