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The map label for this gene is ttdT

Identifier: 209400804

GI number: 209400804

Start: 4050638

End: 4052101

Strand: Direct

Name: ttdT

Synonym: ECH74115_4375

Alternate gene names: 209400804

Gene position: 4050638-4052101 (Clockwise)

Preceding gene: 209400059

Following gene: 209396168

Centisome position: 72.7

GC content: 55.4

Gene sequence:

>1464_bases
ATGAAACCTTCCACTGAATGGTGGCGATACCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTAATTCCGGTTCCCGC
AGGGCTGGAGAGTCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTAATCGTTGGACTGATCCTCGAACCCGTGCCGG
GTGCCGTGGTGGCGATGGTGGGTATCTCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCT
CAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTT
CGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTGGGGCGACGTATCGCGCTGATTCTGGTGAAAAAGATGG
GGCATCGCACGCTGTTTCTTGGCTATGCGGTGATGTTCTCCGAGCTGATCCTGGCACCTGTCACACCGTCCAACTCGGCG
CGTGGTGCGGGGATTATCTATCCCATCATCCGTAACCTGCCGCCGCTCTATCAATCACAACCAAACGACAGCAGTTCGCG
CAGTATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCCTGACGGCGATGGCTC
CTAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTG
CCGCTCAGTATTTTACTGGTCCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCTGTACTGAAGTCAGGCGATCA
GGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGG
TAGGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCGCTGATG
CTGCTTCTGCGCATTATCAGTTGGGACGATATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCT
TATCACCCTCGCGACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTT
ATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTTGCCAGCGCCACGGCGTAT
ACCTCCGCTCTCGCACCGATGATGATTGCCGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGT
TGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATC
TGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTG
TGGATGCCCGTGGTGTTGCTTTAA

Upstream 100 bases:

>100_bases
CCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCGCCCCCTCTCC
TTTTTCGCAGGCATAACACG

Downstream 100 bases:

>100_bases
GTAAGGACGGTGCCGGAAGTAACTCTCCGGCACCCGATAGTTTTACGCAGCCGGTAACTCCGCCAGCGGCCAGCGCGGAC
GCACGCTAACGCCGAGATCC

Product: anion transporter

Products: tartrate [Cytoplasm]; succinate [Periplasm] [C]

Alternate protein names: Tartrate carrier; Tartrate transporter

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVIVGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLA
QPGFKFTAKSLSWAVSGFSNSVIWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLLIGLMKSASHATLSWGDWFLGML
PLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETELQAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALM
LLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRYFFASATAY
TSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLVLLVITGLL
WMPVVLL

Sequences:

>Translated_487_residues
MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVIVGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLA
QPGFKFTAKSLSWAVSGFSNSVIWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLLIGLMKSASHATLSWGDWFLGML
PLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETELQAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALM
LLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRYFFASATAY
TSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLVLLVITGLL
WMPVVLL
>Mature_487_residues
MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVIVGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLA
QPGFKFTAKSLSWAVSGFSNSVIWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLLIGLMKSASHATLSWGDWFLGML
PLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETELQAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALM
LLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRYFFASATAY
TSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLVLLVITGLL
WMPVVLL

Specific function: Catalyzes the uptake of tartrate in exchange for intracellular succinate. Essential for anaerobic L-tartrate fermentation

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily

Homologues:

Organism=Escherichia coli, GI1789444, Length=487, Percent_Identity=99.5893223819302, Blast_Score=948, Evalue=0.0,
Organism=Escherichia coli, GI1786829, Length=471, Percent_Identity=45.0106157112527, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1786986, Length=480, Percent_Identity=34.1666666666667, Blast_Score=265, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTDT_ECO57 (Q8XBK4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85967
- PIR:   B91122
- RefSeq:   NP_289638.1
- RefSeq:   NP_311973.1
- EnsemblBacteria:   EBESCT00000028189
- EnsemblBacteria:   EBESCT00000055857
- GeneID:   916226
- GeneID:   961221
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4416
- KEGG:   ecs:ECs3946
- GeneTree:   EBGT00050000009673
- HOGENOM:   HBG321139
- OMA:   TSSMFLS
- ProtClustDB:   CLSK872279
- BioCyc:   ECOL83334:ECS3946-MONOMER
- InterPro:   IPR001898
- TIGRFAMs:   TIGR00785

Pfam domain/function: PF00939 Na_sulph_symp

EC number: NA

Molecular weight: Translated: 52880; Mature: 52880

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11a81a08)-; HASH(0x11998f6c)-; HASH(0x11ac29d8)-; HASH(0x11970880)-; HASH(0x11acaac4)-; HASH(0x11a0cef0)-; HASH(0x10c01808)-; HASH(0xf4f6130)-; HASH(0x115f4320)-; HASH(0xf4f6328)-; HASH(0xf4e9ac8)-; HASH(0xf3c8f18)-; HASH(0x11a054e8)-; HASH(0x116b54bc)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVIVGLILEPVPGAVVAMV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSVIWLIFAAFMFGTGYEKTG
HHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSARGAGIIYPIIRNLPPLYQSQ
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLLIGLMKSASHATLSWGDWFLGML
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHH
PLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETELQAMGPLCSREKRMLGLMVGALVLW
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHH
SWFGKLLAGSLSGYSPTMVMVALIVVFYLLRYFFASATAYTSALAPMMIAAALAMPEIPL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
PVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLVLLVITGLL
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WMPVVLL
HHHHHHH
>Mature Secondary Structure
MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVIVGLILEPVPGAVVAMV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSVIWLIFAAFMFGTGYEKTG
HHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSARGAGIIYPIIRNLPPLYQSQ
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLLIGLMKSASHATLSWGDWFLGML
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHH
PLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETELQAMGPLCSREKRMLGLMVGALVLW
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNNTGFI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHH
SWFGKLLAGSLSGYSPTMVMVALIVVFYLLRYFFASATAYTSALAPMMIAAALAMPEIPL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
PVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLVLLVITGLL
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WMPVVLL
HHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: tartrate [Periplasm]; succinate [Cytoplasm] [C]

Specific reaction: tartrate [Periplasm] + succinate [Cytoplasm] = tartrate [Cytoplasm] + succinate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796