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The map label for this gene is uhpB [H]

Identifier: 209399895

GI number: 209399895

Start: 447360

End: 448901

Strand: Reverse

Name: uhpB [H]

Synonym: ECH74115_0437

Alternate gene names: 209399895

Gene position: 448901-447360 (Counterclockwise)

Preceding gene: 209400645

Following gene: 209400657

Centisome position: 8.06

GC content: 53.7

Gene sequence:

>1542_bases
ATGTCGCGCAATCTTCGTCATTGGGTGATTTTGCTGTTTATTGTGCTAGCCTGGGGAACCGGCTGGCTGATGCTGTGGAC
GCTCAGCTTTTATCTGACCAATAACGGTCAACAGGCGGTGTTGTTTCTCCCGCAGGGAGTTTATCTCGCCCTTGTTATTT
TGCTCTCTCGCCGCTACTGGCCTGCGTTGGTGCTCCCCCCTTTGCTGATGATGTTCTGGCTCCATAGTGAACAGCTATTG
AACGGTTATCTCATGCTGGCAACGCCGGTTATTAGCCTGTTTCCTGCCCTCCTTGCGCAAAACTTCTGGCACCGTTTTCC
ACTTTACTGGCAGCGTCTGACGTTGCTGTTAGCCACTGTCACGGCAGCTTCATTGTTGAATACCGCCCTGCTCTCCCCTT
TTATGTCAGGCCCAATAATGCTACCGGGTTTGACCTCATTTACCGGCGGCGTATTACTGACGCCGTTTGTCTATCTTATC
TTTGAGTTTCTGCGCCAGCAGCATCGCTACCAGCTCCTGGGACTCGATACGCATAATCCCCCCTTGCGTACCTCTTTAAT
CATCTGGTGTAGCCTGTTTTTTATTATTGGTATCGGCACTCAGATGGTGCTGTCACCTGAAATTGAACGTCTGCTGTTGA
TCGTGGTCTTTTTGCCCAATGTGGTGATGGCGTGGAAATTTGGCTGGCAAGGTGGCGTACTCTCCGGGCTTTTGGGCAGC
ATGATGATCACCATCGCCCGCCAGATTGGCGTGGGCTTTAGTAACCTGGTGGAGCTGGAGATTTTCCTCGCGACCCAGGC
CTTGCTGGGAATTGGCCTGGGCATTGCCATCAGTCGCCAGCAACATCTGGCGCTAAACCTGCACCATTACCGACAACGGC
TGGAGGCCGAGCTGGCAGCGCGGCGGGCATTGGCGGAAAAGCTGATCCACACCGAGGAGGACACGCGAAAAAACCTGGCG
CGAGAGCTGCACGACGAAATCGGTCAGAACATCACCGCCATTCAGATCCAGTCGCAACTGGTGAAGCGGGCGCGTGATCC
GGCGCAAATTCAATCCGCAGCCAGCCAAATCAATGAGCTCGCCCGCCGCATTCATCTCTCCACCCGCCAATTGCTGCGCC
AGTTGCGGCCTCCCGCGCTTGATGAACTGACATTTCGCGAAGCGTTGCTGCATCTGATTAATGAATTTGCCTTTAGTGAA
CGCGGCATTCATTGCCAGTTTGCCTATCAACTGAACTCCACACCAGAAAACGAAACGGTGCGCTTTACCTTGTATCGGTT
ATTGCAGGAGTTGCTCAATAACATCTGTAAACACGCCGAAGCCAGCGAAGTGACGATTATCCTGCGCCAGCAGGGAGAGG
TATTGCATCTGGAAGTCAGCGACAACGGTGTGGGGATCGCCAGCGGGAAAATGGCTGGATTTGGCATTCAGGGGATGCGT
GAGCGCGTCAGCGCCCTGGGCGGCGACTTAACGCTCGAAAAACAGCACGGCACGCGGGTAATTGTTAACTTGCCCACATT
TTTGCAACAAATCGCCGGATAA

Upstream 100 bases:

>100_bases
TACATGTGCATCGCGCCAATGTGCTGGGAAAACTGAACTGCCATAGCACCATTGAACTGGTACATTTTGCGCTCGACCAC
CATCTGCTGGCGGGGCATTA

Downstream 100 bases:

>100_bases
CCAGGAAAAAGTCTTAGGTATCCCGGATTTTCTCTCATCCCCTTTCACTTTCATTTTCATATAGTCAGCAACATGGAGAC
AACCATGCACGCGCGTTCAG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLPQGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLL
NGYLMLATPVISLFPALLAQNFWHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLA
RELHDEIGQNITAIQIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSE
RGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQQGEVLHLEVSDNGVGIASGKMAGFGIQGMR
ERVSALGGDLTLEKQHGTRVIVNLPTFLQQIAG

Sequences:

>Translated_513_residues
MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLPQGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLL
NGYLMLATPVISLFPALLAQNFWHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLA
RELHDEIGQNITAIQIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSE
RGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQQGEVLHLEVSDNGVGIASGKMAGFGIQGMR
ERVSALGGDLTLEKQHGTRVIVNLPTFLQQIAG
>Mature_512_residues
SRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLPQGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLN
GYLMLATPVISLFPALLAQNFWHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLIF
EFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGSM
MITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLAR
ELHDEIGQNITAIQIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSER
GIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQQGEVLHLEVSDNGVGIASGKMAGFGIQGMRE
RVSALGGDLTLEKQHGTRVIVNLPTFLQQIAG

Specific function: Member of the two-component regulatory system uhpB/uhpA involved in the regulation of the uptake of hexose phosphates. UhpB functions as a membrane-associated protein kinase that phosphorylates uhpA in response to environmental signals [H]

COG id: COG3851

COG function: function code T; Signal transduction histidine kinase, glucose-6-phosphate specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=502, Percent_Identity=34.8605577689243, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1787474, Length=228, Percent_Identity=29.8245614035088, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788812, Length=204, Percent_Identity=28.921568627451, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR007895
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3; PF05231 MASE1 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57989; Mature: 57858

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLPQGVYLALVILLSRRYW
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCCC
PALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNFWHRFPLYWQRLTLLLATV
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLIFEFLRQQHRYQLLGLDTHNP
HHHHHHHHHHHHHHHCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
PLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
CHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHH
RRALAEKLIHTEEDTRKNLARELHDEIGQNITAIQIQSQLVKRARDPAQIQSAASQINEL
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHH
ARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSERGIHCQFAYQLNSTPENETV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHH
RFTLYRLLQELLNNICKHAEASEVTIILRQQGEVLHLEVSDNGVGIASGKMAGFGIQGMR
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHH
ERVSALGGDLTLEKQHGTRVIVNLPTFLQQIAG
HHHHHCCCCEEEECCCCCEEEEECHHHHHHHCC
>Mature Secondary Structure 
SRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLPQGVYLALVILLSRRYW
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCCC
PALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNFWHRFPLYWQRLTLLLATV
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLIFEFLRQQHRYQLLGLDTHNP
HHHHHHHHHHHHHHHCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
PLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFLPNVVMAWKFGWQGGVLSGLLGS
CHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MMITIARQIGVGFSNLVELEIFLATQALLGIGLGIAISRQQHLALNLHHYRQRLEAELAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHH
RRALAEKLIHTEEDTRKNLARELHDEIGQNITAIQIQSQLVKRARDPAQIQSAASQINEL
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHH
ARRIHLSTRQLLRQLRPPALDELTFREALLHLINEFAFSERGIHCQFAYQLNSTPENETV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHH
RFTLYRLLQELLNNICKHAEASEVTIILRQQGEVLHLEVSDNGVGIASGKMAGFGIQGMR
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHH
ERVSALGGDLTLEKQHGTRVIVNLPTFLQQIAG
HHHHHCCCCEEEECCCCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3301805; 1569007; 7686882; 9278503 [H]