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The map label for this gene is uhpA [H]

Identifier: 209400645

GI number: 209400645

Start: 448901

End: 449530

Strand: Reverse

Name: uhpA [H]

Synonym: ECH74115_0438

Alternate gene names: 209400645

Gene position: 449530-448901 (Counterclockwise)

Preceding gene: 209399774

Following gene: 209399895

Centisome position: 8.07

GC content: 53.65

Gene sequence:

>630_bases
ATGATTCGGGTAGTGCTGGTGGATGACCATGTTGTGGTGCGCTCTGGCTTCGCACAATTACTCTCTCTGGAAGACGATCT
GGAGGTCATTGGTCAGTACAGTAGTGCCGCGCAGGCATGGTCGGCATTAATCCGTGATGACGTCAATGTTGCCGTCATTG
ATATTGCCATGCCGGACGAAAACGGCCTCAGTTTGCTTAAGCGCCTGCGTGCACAAAAACCGCAATTTCGCGCCATTATT
CTTAGCATTTATGACGCGCCAATCTTTGTGCAAAGCGCGCTGGATGCCGGTGCCAGCGGGTATTTAACCAAACGCTGTGG
GCCGGAAGAGTTAGTACAGGCGGTGCGCTCGGTCGGGCTGGGCGGGCATTATCTCTGTGCCGATGCGATTCGGGCTTTAC
GCGGCGGCGGGCAACCCGCCCAGGCGCTGGAAATTCTCACCCCACGAGAACGTGAAGTGTTTGAGTTACTGGTCAAAGGA
GACAGCGTTAAAGAGATCGCCTTTAAACTCGAACTCAGTCATAAAACGGTACATGTGCATCGCGCCAATGTGCTGGGAAA
ACTGAACTGCCATAGCACCATTGAACTGGTACATTTTGCGCTCGACCACCATCTGCTGGCGGGGCATTAA

Upstream 100 bases:

>100_bases
TTGCCATTTTGTTACATCCATTCCATATCTGTCGCTGGTTGATTGCCAGCGCCGCGCCGTGATGCATGATAGGAGAATTA
GATAATAAGAGAAGAAAAGT

Downstream 100 bases:

>100_bases
TGTCGCGCAATCTTCGTCATTGGGTGATTTTGCTGTTTATTGTGCTAGCCTGGGGAACCGGCTGGCTGATGCTGTGGACG
CTCAGCTTTTATCTGACCAA

Product: transcriptional regulatory protein UhpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALIRDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAII
LSIYDAPIFVQSALDAGASGYLTKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH

Sequences:

>Translated_209_residues
MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALIRDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAII
LSIYDAPIFVQSALDAGASGYLTKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH
>Mature_209_residues
MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALIRDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAII
LSIYDAPIFVQSALDAGASGYLTKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH

Specific function: Appears to be the positive activator of uhpT transcription [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=203, Percent_Identity=48.7684729064039, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=32.5358851674641, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788521, Length=195, Percent_Identity=36.4102564102564, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=27.3170731707317, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787473, Length=184, Percent_Identity=33.1521739130435, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788712, Length=199, Percent_Identity=25.1256281407035, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=25.8706467661692, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22839; Mature: 22839

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALIRDDVNVAVIDIAMPDE
CEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NGLSLLKRLRAQKPQFRAIILSIYDAPIFVQSALDAGASGYLTKRCGPEELVQAVRSVGL
CHHHHHHHHHCCCCCHHEEEEEHHCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCC
GGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKGDSVKEIAFKLELSHKTVHVH
CCCHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCHHEEEEEEEECCCEEEEE
RANVLGKLNCHSTIELVHFALDHHLLAGH
ECCCEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALIRDDVNVAVIDIAMPDE
CEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
NGLSLLKRLRAQKPQFRAIILSIYDAPIFVQSALDAGASGYLTKRCGPEELVQAVRSVGL
CHHHHHHHHHCCCCCHHEEEEEHHCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCC
GGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKGDSVKEIAFKLELSHKTVHVH
CCCHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCHHEEEEEEEECCCEEEEE
RANVLGKLNCHSTIELVHFALDHHLLAGH
ECCCEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]