Click here to switch to the map view.

The map label for this gene is eptA [H]

Identifier: 209397281

GI number: 209397281

Start: 1331574

End: 1333193

Strand: Reverse

Name: eptA [H]

Synonym: ECH74115_1318

Alternate gene names: 209397281

Gene position: 1333193-1331574 (Counterclockwise)

Preceding gene: 209400236

Following gene: 209398348

Centisome position: 23.93

GC content: 32.84

Gene sequence:

>1620_bases
ATGATTAAAAAAATCAGTATTAATTTTCTTTTCTTGATGTTAATGATAGATGTTGTTTTTGCCACATTGTTCAATATCCC
TGTCTGGATGCATCTGTTTAATATTATTAATAACCTTGATGGTGTGAAACTTGGATTTATTATATCTCTTCCAGTCTTTC
TGATATCTGCATTAAATTTTGTTTTTACACCGTTCTCATTCAGGTATATATTTAAACCTTTTTTTTGTATTCTGTTTATC
TGTAGTTCCATTGTTACATATGCCACCATGAAATATGGTGTGCAGTTTGATAAAACAATGATGCAGAATATTTTTGAAAC
AAATGCAGGTGAAATGACATCATATTTCAATATGTCTGTTGTACTGTGGTTTTTATTTACGGGTATACTTCCATGTGGAC
TATTGTTGTTAGTAAATATCCGTTATCCTGAGACATGGATAAAGGGTATTACATATCGTTTAATATCAATGTTTGCATCT
CTCTTGATTATATTTGCGATTGCGTTTTTCTTTTATAAGGATTATGCATCAGTTGGCAGAAACAACTCTTCATTAAATAA
AGAAATAATACCTACTAATTATATCTACAGTGGGTTTAAATATGTAAGGGATTTTTTTGTTTCTCCTGGCGAGTTTCGTC
AGACTGGAACGGATGCATCCCGCACAATAAATGAAAAACAAAAGCCGGTAATTATGTTTTTAGTTGTCGGGGAGACAGCA
AGAAGTCAAAACTATGCTCTGAATGGATATTCTCGTGGTACCAATGATTTTACAAAGAAATATAATGAACTTATATCCTT
TCATAATGTGCAGTCGTGTGGCACATCCACAGCCATATCTGTTCCATGCATGTTTTCAGATATGAAGCGAAAAGAGTTCA
ATTCCCGCAAAGCGGTAAATTCGGAAAATGTACTGGATATTCTGTACAGAACGGGGGTAAATTTACTCTGGATTGAAAAT
GACGGGGGATGTAAAGGTGTATGTAAGCGAATCCCTACAATTAATATTGAGCCATCAAATTCAGATAATACATTATGCAA
GAAAAACTCATGTTATGATGAGGTTATGCTAAAAAATATTGATGAGTATATAAATAATAACTCAGAGGATAAACTTATCG
TATTTCATTTGATGGGTAGTCATGGTCCTACCTACTATCTCCGTTATCCTGAGTCACATAAATATTTTAAACCAACATGT
GATCGTAGTGATATCGAAAATTGTACGCATGAGCAATTGATTAATACCTATGATAATACAATTCGCTATACTGATTATAT
AATATCAAAATTAATTGACAAGCTTATCGAATATAAAGATGAGTATGATACTGTTTTATTATATGTCTCTGACCATGGAG
AATCTCTGGGAGAAAATGGATTATACTTACATGGAACACCATATAATGTTGCTCCAGCTGAGCAAACGCATGTGCCTCTT
ATTACCTGGATGTCTCCGGGGTTCGTTTCCTCTAAAAAAATTGATTTGAATTGTTTGTCAGAACATGCTCTAAACAGAAC
AGTAAGTCATGATAATATATTTTCATCGTTACTGGGGTTATGGAATGTAAATACTTCAGTTTATAATAAAGAAGATGATA
TTATATCCTCCTGCAGATAG

Upstream 100 bases:

>100_bases
AGTTTGACCTGGTATAAATCAGTATTCACTTTACCAGGTTTTATAAAAATATATCACTTTTAGATACGGACAATTATAGG
CTCTAATTAATGGGGGTAAG

Downstream 100 bases:

>100_bases
ATACTGACATTTTTCTGAGGATAAGAGGTGAATACCTCTTATCCTTAAGTAAATATTAACCAGGTAACTGTGAATAATTT
AGATGTTGGGCGTTATGAAA

Product: integral membrane protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKLGFIISLPVFLISALNFVFTPFSFRYIFKPFFCILFI
CSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFAS
LLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETA
RSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIEN
DGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTC
DRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPL
ITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR

Sequences:

>Translated_539_residues
MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKLGFIISLPVFLISALNFVFTPFSFRYIFKPFFCILFI
CSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFAS
LLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETA
RSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIEN
DGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTC
DRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPL
ITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR
>Mature_539_residues
MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKLGFIISLPVFLISALNFVFTPFSFRYIFKPFFCILFI
CSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFAS
LLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETA
RSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIEN
DGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTC
DRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPL
ITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=495, Percent_Identity=42.8282828282828, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI87082286, Length=264, Percent_Identity=31.4393939393939, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI87082223, Length=431, Percent_Identity=24.3619489559165, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790392, Length=520, Percent_Identity=22.3076923076923, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61976; Mature: 61976

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKLGFIISLPVFLISALNF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
VLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFASLLIIFAIAFFFYKDYASVGR
HHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETA
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCCCEEEEEEECCCC
RSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVN
CCCCCEECCCCCCCHHHHHHHHHHHHHHCHHHCCCCCEEECCHHHHHHHHHHHHHHHCCC
SENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNI
CCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
DEYINNNSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHEQLINTYDNT
HHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCH
IRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCE
ITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR
EEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKLGFIISLPVFLISALNF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
VLWFLFTGILPCGLLLLVNIRYPETWIKGITYRLISMFASLLIIFAIAFFFYKDYASVGR
HHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETA
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCCCEEEEEEECCCC
RSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVN
CCCCCEECCCCCCCHHHHHHHHHHHHHHCHHHCCCCCEEECCHHHHHHHHHHHHHHHCCC
SENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNI
CCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHH
DEYINNNSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHEQLINTYDNT
HHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCH
IRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCE
ITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR
EEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]