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The map label for this gene is pet [H]

Identifier: 209400236

GI number: 209400236

Start: 1339016

End: 1339141

Strand: Reverse

Name: pet [H]

Synonym: ECH74115_1327

Alternate gene names: 209400236

Gene position: 1339141-1339016 (Counterclockwise)

Preceding gene: 209399414

Following gene: 209397281

Centisome position: 24.03

GC content: 47.62

Gene sequence:

>126_bases
ATGAATGTTGGCCTGAATGCGGAAATTCGCGACAACATCCGCTTCGGTCTGGAGTTTGAAAAATCGGCATCTGGTAAGTA
CAACGTGGATAACGCGGTGAACGCCAACTTCCGTTATTCCTTCTGA

Upstream 100 bases:

>100_bases
TATCGGTTATCAGTTTGACCTGCTGGCTAACGGTGAAACCGTACTGCGTGATGCATCCGGCGAAAAACGCATCAAGGGTG
AAAAAGACGGTCGTATGCTC

Downstream 100 bases:

>100_bases
TAAAAACAAGGGGCCGCCTGCCGGCCCTGATACAGAGGATGTTGTGATTACTTACCCAGCATGCCCTGCTCTTCGATAAA
GGCGATGATGTTTTGCAGAC

Product: serine proteAse eata

Products: NA

Alternate protein names: Plasmid-encoded toxin pet [H]

Number of amino acids: Translated: 41; Mature: 41

Protein sequence:

>41_residues
MNVGLNAEIRDNIRFGLEFEKSASGKYNVDNAVNANFRYSF

Sequences:

>Translated_41_residues
MNVGLNAEIRDNIRFGLEFEKSASGKYNVDNAVNANFRYSF
>Mature_41_residues
MNVGLNAEIRDNIRFGLEFEKSASGKYNVDNAVNANFRYSF

Specific function: Serine protease with enterotoxic and cytotoxic activity. Internalization into the host cell is required for the induction of cytopathic effects. However, the serine activity is not necessary for secretion and internalization into the host cell [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Serine protease pet translocator:Cell outer membrane; Multi-pass membrane protein. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S6 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005546
- InterPro:   IPR006315
- InterPro:   IPR012332
- InterPro:   IPR011050
- InterPro:   IPR000710 [H]

Pfam domain/function: PF03797 Autotransporter; PF02395 Peptidase_S6 [H]

EC number: NA

Molecular weight: Translated: 4672; Mature: 4672

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVGLNAEIRDNIRFGLEFEKSASGKYNVDNAVNANFRYSF
CCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MNVGLNAEIRDNIRFGLEFEKSASGKYNVDNAVNANFRYSF
CCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9632580 [H]