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The map label for this gene is acpT

Identifier: 209396708

GI number: 209396708

Start: 4450651

End: 4451238

Strand: Direct

Name: acpT

Synonym: ECH74115_4815

Alternate gene names: 209396708

Gene position: 4450651-4451238 (Clockwise)

Preceding gene: 209395848

Following gene: 209399938

Centisome position: 79.87

GC content: 56.63

Gene sequence:

>588_bases
ATGTATCGGATAGTTCTGGGGAAAGTTTCGACCTTAAGCGCAGCTCCACTGCCACCGGGTTTACGCGAGCAAGCACCGCA
AGGTCCACGACGCGAACGCTGGCTGGCGGGGCGTGCATTGCTTTCGCACACGCTTTCCCCGCTACCGGAGATCATCTATG
GCGAACAAGGCAAACCTGCATTTGCGCCGGAAACGCCGCTATGGTTCAACTTAAGCCATAGCGGTGACGATATCGCCCTG
CTGTTGAGTGATGAAGGCGAAGTCGGCTGCGATATCGAAGTGATTCGCCCGCGCGCCAACTGGCGCTGGCTGGCGAACGC
CGTGTTCAGCCTCGGGGAACATGCTGAGATGGACGCCGTGCATCCTGATCAGCAACTGGAAATGTTCTGGCGCATCTGGA
CGCGCAAAGAAGCCATCGTTAAACAGCGTGGTGGCAGCGCCTGGCAAATCGTCAGCGTAGACAGCACCTATCACTCCTCG
CTGTCAGTCAGCCATTGTCAGCTAGAAAATTTAAGCCTGGCGATCTGCACCCCTACTCCCTTTACGCTCACCGCCGACAG
TGTGCAATGGATCGATTCAGTTAACTGA

Upstream 100 bases:

>100_bases
ATGCATGAAGCCCGCAAGGTTGATTGTGAGTTTTTGCAGAGTAACAACTTTGCCTTTGGCGGCATTAATACATCCATCAT
CATTAAACGTTGGCCCTGAT

Downstream 100 bases:

>100_bases
TCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTC
CGCCATCTATTTTAATCCAT

Product: holo-(acyl carrier protein) synthase 2

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN

Sequences:

>Translated_195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN
>Mature_195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN

Specific function: May be involved in an alternative pathway for phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The native apo-protein substrate is unknown. Is able to functionally replace AcpS in vivo but only when expressed at high levels

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family

Homologues:

Organism=Escherichia coli, GI1789886, Length=195, Percent_Identity=99.4871794871795, Blast_Score=399, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACPT_ECO57 (Q8X5U4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F86017
- PIR:   F91171
- RefSeq:   NP_290041.1
- RefSeq:   NP_312369.1
- ProteinModelPortal:   Q8X5U4
- SMR:   Q8X5U4
- EnsemblBacteria:   EBESCT00000028987
- EnsemblBacteria:   EBESCT00000055526
- GeneID:   915791
- GeneID:   961237
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4867
- KEGG:   ecs:ECs4342
- GeneTree:   EBGT00050000011504
- HOGENOM:   HBG417151
- OMA:   PLPEIVY
- ProtClustDB:   PRK10351
- BioCyc:   ECOL83334:ECS4342-MONOMER
- InterPro:   IPR008278

Pfam domain/function: PF01648 ACPS; SSF56214 4-PPT_transf

EC number: NA

Molecular weight: Translated: 21738; Mature: 21738

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPTP
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECEEEECCCEEEEECCCC
FTLTADSVQWIDSVN
CEEECCCHHHHCCCC
>Mature Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPTP
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECEEEECCCEEEEECCCC
FTLTADSVQWIDSVN
CEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796