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The map label for this gene is cusF

Identifier: 209396059

GI number: 209396059

Start: 682941

End: 683273

Strand: Direct

Name: cusF

Synonym: ECH74115_0654

Alternate gene names: 209396059

Gene position: 682941-683273 (Clockwise)

Preceding gene: 209399978

Following gene: 209400334

Centisome position: 12.26

GC content: 45.35

Gene sequence:

>333_bases
ATGAAAAAAGCACTGCAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCA
TGAAACCATGAGCGAAGCACAACCACAGGTCATTAGTGCCACCGGCGTGGTAAAAGGTTTTGATCTGGAAAGCAAAAAAA
TCACCATCCATCACGATCCGATTGCTGCCGTGAACTGGCCGGAGATGACCATGCGCTTTACCATCACCCCGCAGACGAAA
ATGAGTGGAATTAAAACCGGCGACAAAGTGGCGTTTAATTTTGTCCAGCAGGGCAACCTTTCTTTATTACAGGATATTAA
AGTCAGCCAGTAA

Upstream 100 bases:

>100_bases
TACTTGACCTGAATTATGCCCGTCAGGTCAACGAAATTTCTTTATATACCGCACTTGGTGGCGGTTGGCAGCAATAATTT
TTAACTCCAGGAGAAAATAA

Downstream 100 bases:

>100_bases
CCCAGGTTTAATGAGATGAAAAAAATCGCGCTTATTATCGGCAGCATGATCGCGGGCGGTATTATTTCTGCGGCAGGTTT
TACCTGGTTTGCAAAGGCGG

Product: periplasmic copper-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVVKGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTK
MSGIKTGDKVAFNFVQQGNLSLLQDIKVSQ

Sequences:

>Translated_110_residues
MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVVKGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTK
MSGIKTGDKVAFNFVQQGNLSLLQDIKVSQ
>Mature_110_residues
MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVVKGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTK
MSGIKTGDKVAFNFVQQGNLSLLQDIKVSQ

Specific function: Part of a cation efflux system that mediates resistance to copper and silver. Binds one copper per polypeptide

COG id: COG5569

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786786, Length=110, Percent_Identity=98.1818181818182, Blast_Score=222, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CUSF_ECO57 (Q8XBY2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90705
- PIR:   F85555
- RefSeq:   NP_286298.1
- RefSeq:   NP_308638.1
- ProteinModelPortal:   Q8XBY2
- SMR:   Q8XBY2
- EnsemblBacteria:   EBESCT00000028303
- EnsemblBacteria:   EBESCT00000059076
- GeneID:   916969
- GeneID:   957570
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0712
- KEGG:   ecs:ECs0611
- GeneTree:   EBGT00050000009795
- HOGENOM:   HBG648484
- OMA:   MHEQPAA
- ProtClustDB:   PRK09838
- BioCyc:   ECOL83334:ECS0611-MONOMER
- InterPro:   IPR021647

Pfam domain/function: PF11604 CusF_Ec

EC number: NA

Molecular weight: Translated: 12213; Mature: 12213

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVVKGFDLESKKITIHHDP
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEECCCCCCCEEEEECCC
IAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSLLQDIKVSQ
EEEECCCCEEEEEEECCCHHCCCCCCCCHHEEEECCCCCHHEECCCCCCC
>Mature Secondary Structure
MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVVKGFDLESKKITIHHDP
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCEEECCCCCCCEEEEECCC
IAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSLLQDIKVSQ
EEEECCCCEEEEEEECCCHHCCCCCCCCHHEEEECCCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796