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The map label for this gene is pckA

Identifier: 209696326

GI number: 209696326

Start: 3171402

End: 3173027

Strand: Direct

Name: pckA

Synonym: VSAL_I2928

Alternate gene names: 209696326

Gene position: 3171402-3173027 (Clockwise)

Preceding gene: 209696325

Following gene: 209696327

Centisome position: 95.38

GC content: 41.02

Gene sequence:

>1626_bases
ATGACTGTAATGGAACACAAAAAGGCTGCACAACTTGATCTTTCTCAACACGGCTTAAGCAACGTGACGGAAGTTCTTCG
TAACCCTAGCTATGACGTACTTTTTGAAGAAGAAACGCGTCCTGACTTAGAAGGTTATGAACGTGGTGTAATGACAGAAC
TTGGCTCTGTTGCGGTAGATACTGGTATTTTTACCGGTCGTTCGCCAAAAGATAAATACATCGTAAAAGATGAAACAACC
AAAGATACCTTGTGGTGGTCTGATCAAGGTAAAAATGACAACAAAGCCATTACCCCTGCAGTCTGGGATGATTTAAAAGC
CCTTGTTACAGAACAACTATCAGGCAAACGTTTGTTTGTTATTGATGGCTTCTGTGGTGCAAACCCTGATACTCGCCTTA
GTGTTCGAGTTATCACGGAAGTCGCATGGCAGGCTCACTTTGTGAAAAATATGTTTATTCGCCCAACGGACGAAGAATTA
GAAAGTTTTGAACCTGATTTTGTCGTCATGAATGGCGCAAAAGCAACCAATCCAAATTACGAAGCACAAGGGTTAAACTC
TGAAAACTTTGTTGCCTTCAACTTAACAGAGCGTGTTCAAATCATCGGTGGTACTTGGTACGGCGGTGAGATGAAAAAAG
GCATGTTCGCAATGATGAATTACTTACTTCCGTTAAAAGGCATTGCATCTATGCACTGTTCAGCTAACGTAGGTAAAGAA
GGCGATGTTGCTGTATTCTTCGGCTTATCAGGTACAGGTAAAACAACCCTATCAACGGATCCAAAACGTCAATTAATTGG
GGATGATGAACACGGTTGGGATGATGATGGTATCTTTAACTTTGAAGGTGGCTGTTACGCGAAAACCATTCGTTTATCTA
AAGAAGCTGAACCGGATATTTTTAATGCCATTCGTCGTGATGCACTATTAGAAAACGTAACCGTTCGCAATGATGGTTCG
ATTGATTTTGATGACGGTTCTAAAACAGAAAATACTCGTGTTTCTTACCCTATCTATCACATTGATAATATCGTTAAACC
GATTTCAAAAGCAGGTCATGCAAACAAAGTTATTTTCTTAACCGCTGATGCATTTGGTGTTCTACCTCCTGTTGCTAAAT
TAACGCCAGAACAAACGAAATATCATTTCTTATCAGGTTTTACCGCTAAATTAGCCGGTACTGAACGTGGCATTACAGAA
CCAACACCAACATTCTCTGCTGCTTTTGGTGCTGCGTTCTTAACACTACACCCAACACAATACGCAGAAGTATTGGTGAA
ACGTATGGAAGCCGCTGGTGCAGAAGCTTACATCGTAAACACAGGTTGGAACGGCACAGGCAAACGTATCTCAATTCAAG
ATACGCGTGGCATCATTGATGCAATTTTAGATGGTTCTATTGATAATGTAGAAACGAAAAACATTCCAGTCTTTAACCTT
GAAGTACCGACATCACTACCAGGTGTTGATACCTCAATTCTTGACCCTCGTGATACATATACAGACCCATTACAGTGGGA
CAGTAAAGCGGAAGATTTAGCACAACGCTTCATCAAAAACTTTGCTCAATACACAGATAATGACGAAGGGAAAGCGCTTG
TAAAAGCAGGTCCTCAGCTGGATTAA

Upstream 100 bases:

>100_bases
CCTGTTTTCAATTTACCGCGTTGGTAGCATGGGTAGCAGTAAACAATATTAAAATAATTAGAATATTAAAATCCCTACAA
AATTCCTTTAAGGAGCACCT

Downstream 100 bases:

>100_bases
TCCGCCTTACATGTAAATCGTTTTTATTTAAGCCCTTCATTCGAAGGGCTTTTTTTACCCATAAATAGGCCAATAACAAT
AAAGCTTTATTCTATTTTCC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MTVMEHKKAAQLDLSQHGLSNVTEVLRNPSYDVLFEEETRPDLEGYERGVMTELGSVAVDTGIFTGRSPKDKYIVKDETT
KDTLWWSDQGKNDNKAITPAVWDDLKALVTEQLSGKRLFVIDGFCGANPDTRLSVRVITEVAWQAHFVKNMFIRPTDEEL
ESFEPDFVVMNGAKATNPNYEAQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGKE
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDIFNAIRRDALLENVTVRNDGS
IDFDDGSKTENTRVSYPIYHIDNIVKPISKAGHANKVIFLTADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITE
PTPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDNVETKNIPVFNL
EVPTSLPGVDTSILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNDEGKALVKAGPQLD

Sequences:

>Translated_541_residues
MTVMEHKKAAQLDLSQHGLSNVTEVLRNPSYDVLFEEETRPDLEGYERGVMTELGSVAVDTGIFTGRSPKDKYIVKDETT
KDTLWWSDQGKNDNKAITPAVWDDLKALVTEQLSGKRLFVIDGFCGANPDTRLSVRVITEVAWQAHFVKNMFIRPTDEEL
ESFEPDFVVMNGAKATNPNYEAQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGKE
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDIFNAIRRDALLENVTVRNDGS
IDFDDGSKTENTRVSYPIYHIDNIVKPISKAGHANKVIFLTADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITE
PTPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDNVETKNIPVFNL
EVPTSLPGVDTSILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNDEGKALVKAGPQLD
>Mature_540_residues
TVMEHKKAAQLDLSQHGLSNVTEVLRNPSYDVLFEEETRPDLEGYERGVMTELGSVAVDTGIFTGRSPKDKYIVKDETTK
DTLWWSDQGKNDNKAITPAVWDDLKALVTEQLSGKRLFVIDGFCGANPDTRLSVRVITEVAWQAHFVKNMFIRPTDEELE
SFEPDFVVMNGAKATNPNYEAQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGKEG
DVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDIFNAIRRDALLENVTVRNDGSI
DFDDGSKTENTRVSYPIYHIDNIVKPISKAGHANKVIFLTADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITEP
TPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDNVETKNIPVFNLE
VPTSLPGVDTSILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNDEGKALVKAGPQLD

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=530, Percent_Identity=80, Blast_Score=909, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=549, Percent_Identity=44.2622950819672, Blast_Score=455, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_ALISL (B6EGP7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264257.1
- GeneID:   6988684
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2928
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59685; Mature: 59553

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMEHKKAAQLDLSQHGLSNVTEVLRNPSYDVLFEEETRPDLEGYERGVMTELGSVAVD
CCCCCCCHHHHCCHHHCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHEE
TGIFTGRSPKDKYIVKDETTKDTLWWSDQGKNDNKAITPAVWDDLKALVTEQLSGKRLFV
CCEECCCCCCCCEEECCCCCCCEEEECCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEE
IDGFCGANPDTRLSVRVITEVAWQAHFVKNMFIRPTDEELESFEPDFVVMNGAKATNPNY
EECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCC
EAQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGKE
CCCCCCCCCEEEEEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDI
CCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCHH
FNAIRRDALLENVTVRNDGSIDFDDGSKTENTRVSYPIYHIDNIVKPISKAGHANKVIFL
HHHHHHHHHHHCCEECCCCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEE
TADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITEPTPTFSAAFGAAFLTLHPTQ
ECCCCCCCCCHHHCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHH
YAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDNVETKNIPVFNL
HHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCEEEE
EVPTSLPGVDTSILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNDEGKALVKAGPQL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
D
C
>Mature Secondary Structure 
TVMEHKKAAQLDLSQHGLSNVTEVLRNPSYDVLFEEETRPDLEGYERGVMTELGSVAVD
CCCCCCHHHHCCHHHCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHEE
TGIFTGRSPKDKYIVKDETTKDTLWWSDQGKNDNKAITPAVWDDLKALVTEQLSGKRLFV
CCEECCCCCCCCEEECCCCCCCEEEECCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEE
IDGFCGANPDTRLSVRVITEVAWQAHFVKNMFIRPTDEELESFEPDFVVMNGAKATNPNY
EECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCC
EAQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLKGIASMHCSANVGKE
CCCCCCCCCEEEEEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDVAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPDI
CCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCHH
FNAIRRDALLENVTVRNDGSIDFDDGSKTENTRVSYPIYHIDNIVKPISKAGHANKVIFL
HHHHHHHHHHHCCEECCCCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEE
TADAFGVLPPVAKLTPEQTKYHFLSGFTAKLAGTERGITEPTPTFSAAFGAAFLTLHPTQ
ECCCCCCCCCHHHCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHH
YAEVLVKRMEAAGAEAYIVNTGWNGTGKRISIQDTRGIIDAILDGSIDNVETKNIPVFNL
HHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCEEEE
EVPTSLPGVDTSILDPRDTYTDPLQWDSKAEDLAQRFIKNFAQYTDNDEGKALVKAGPQL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA