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The map label for this gene is pykF [H]

Identifier: 209696108

GI number: 209696108

Start: 2924806

End: 2926218

Strand: Direct

Name: pykF [H]

Synonym: VSAL_I2701

Alternate gene names: 209696108

Gene position: 2924806-2926218 (Clockwise)

Preceding gene: 209696107

Following gene: 209696109

Centisome position: 87.96

GC content: 41.9

Gene sequence:

>1413_bases
ATGAAAAAGACCAAAATCGTTTGTACGATTGGCCCAAAAACTGAATCAGTAGAAAAACTAACTGAATTAGCAAATGCTGG
CATGAACGTAATGCGCCTTAACTTCTCACACGGCGATTTCCAAGAGCACGGCAACCGTATCGATAATCTTCGTCAAGTAA
TGAAGAACACAGGTAAGCAACTTGCTATCCTTCTTGATACTAAAGGTCCTGAAATCCGTACTATCAAACTAGACAACGGC
AATGATGTCGCTCTAGTTGCTGGTCAAGACTTCACATTTACAACTGACGTTTCAGTTGTAGGTAATAAAGACGTTGTTGC
TGTTACTTACCCAGGTTTTGCAAAAGATCTTGTTACTGGTAACACTATCCTTGTTGATGACGGTCTAATTGAAATGGAAG
TAGTTTCGACTACTGAAACTGAAGTTAAATGTAAAGTTCTTAACAACGGTGACCTAGGCGAAAACAAAGGTGTTAACCTT
CCTGGTGTTTCAGTTAAGCTTCCAGCTCTTTCTGAAAAAGATAAAGCTGACCTTAAATTTGGTTGTGAGCAAGGCGTTGA
TTTCGTTGCTGCATCTTTTATTCGTAAAGAAGAAGATGTTAAAGAAATCCGTGCTCTACTTGTTGCTAACGGCGGCGAGC
ACATCCAAATCATCTCTAAGATTGAAAACCAAGAAGGTGTAGATAACTTCGATTCAATCCTAGAAGCTTCTGACGGTATT
ATGGTTGCTCGTGGTGATCTTGGTGTTGAAATCCCTGTTGAAGAAGTAATCTTTGCACAGAAGATGATGATCGAGAAATG
TAATCGCGCACGTAAAGTTGTAATCACTGCAACTCAAATGCTTGATTCTATGATCAAAAACCCACGTCCAACTCGCGCAG
AAGCGGGCGACGTAGCTAACGCAATCCTTGATGGTACAGATGCAGTAATGCTTTCTGGTGAGTCTGCGAAAGGTAAATAC
CCTATCGAAGCTGTGACTATCATGGCGACAATCTGTGCTCGTACAGATGCAGTATTAAAAGCAGAGCTTAGCTCTCGCCT
AGACAGCCCACGTCTACGTATCACTGAAGCGGTATGTAAAGGCGCTGTTGATACAGCAGAGAAACTAAGTGCACCTGTAA
TCGTAGTAGCGACAGAAGCGGGTAAATCAGCTCGTTCTATTCGTAAATACTTCCCAACAGCAAGAATCATTGCCGTAACG
ACTAATACTAAGACAGCAGCTCAACTTTGTCTTTCTAAAGGTATTACACCAATGGTTGTTGATGCTATCGACAGTACTGA
CGCATTCTACCTACGCGGTAAAGAGTTAGCTCTTGAGTCTGGTATTGGCGCTAAAGGCGATATCGTTGTTATGGTTTCTG
GTGCTCTAGTACCATCAGGCACAACTAACACAGCATCTGTTCACGTATTATAA

Upstream 100 bases:

>100_bases
GAAGCAAGAATTTGTTGTTATATTTCAACACTTCATACCATTAAGCTGAGCGTAACTGTGTTTATCATTTTAAATATACT
TATCTGGAGAGATTTCTCAC

Downstream 100 bases:

>100_bases
TTCAGATGAATATATAAAAATTACGTAACAATCAATAAAAGAGAGCTTCGGCTCTCTTTTTTTATGAATTATCATTGGCA
CAAAACATGCAATACGGTAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFQEHGNRIDNLRQVMKNTGKQLAILLDTKGPEIRTIKLDNG
NDVALVAGQDFTFTTDVSVVGNKDVVAVTYPGFAKDLVTGNTILVDDGLIEMEVVSTTETEVKCKVLNNGDLGENKGVNL
PGVSVKLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRALLVANGGEHIQIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PIEAVTIMATICARTDAVLKAELSSRLDSPRLRITEAVCKGAVDTAEKLSAPVIVVATEAGKSARSIRKYFPTARIIAVT
TNTKTAAQLCLSKGITPMVVDAIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFQEHGNRIDNLRQVMKNTGKQLAILLDTKGPEIRTIKLDNG
NDVALVAGQDFTFTTDVSVVGNKDVVAVTYPGFAKDLVTGNTILVDDGLIEMEVVSTTETEVKCKVLNNGDLGENKGVNL
PGVSVKLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRALLVANGGEHIQIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PIEAVTIMATICARTDAVLKAELSSRLDSPRLRITEAVCKGAVDTAEKLSAPVIVVATEAGKSARSIRKYFPTARIIAVT
TNTKTAAQLCLSKGITPMVVDAIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFQEHGNRIDNLRQVMKNTGKQLAILLDTKGPEIRTIKLDNG
NDVALVAGQDFTFTTDVSVVGNKDVVAVTYPGFAKDLVTGNTILVDDGLIEMEVVSTTETEVKCKVLNNGDLGENKGVNL
PGVSVKLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRALLVANGGEHIQIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PIEAVTIMATICARTDAVLKAELSSRLDSPRLRITEAVCKGAVDTAEKLSAPVIVVATEAGKSARSIRKYFPTARIIAVT
TNTKTAAQLCLSKGITPMVVDAIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=47.1311475409836, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=47.1311475409836, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=47.3360655737705, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI32967597, Length=486, Percent_Identity=47.119341563786, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI10835121, Length=486, Percent_Identity=47.119341563786, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=51.0416666666667, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=51.0416666666667, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=46.1864406779661, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=46.1864406779661, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=45.1162790697674, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=77.6595744680851, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=38.1856540084388, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=44.5833333333333, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=44.6280991735537, Blast_Score=343, Evalue=7e-95,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=44.6280991735537, Blast_Score=343, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=44.6280991735537, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=44.6280991735537, Blast_Score=343, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=45.7380457380457, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6324923, Length=482, Percent_Identity=43.3609958506224, Blast_Score=355, Evalue=9e-99,
Organism=Drosophila melanogaster, GI28571814, Length=481, Percent_Identity=47.1933471933472, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24648964, Length=481, Percent_Identity=47.1933471933472, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24648966, Length=443, Percent_Identity=46.7268623024831, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=35.4166666666667, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24646914, Length=301, Percent_Identity=45.514950166113, Blast_Score=221, Evalue=8e-58,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50165; Mature: 50165

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFQEHGNRIDNLRQVMKNTGKQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHHHHHHCCCCE
LAILLDTKGPEIRTIKLDNGNDVALVAGQDFTFTTDVSVVGNKDVVAVTYPGFAKDLVTG
EEEEEECCCCEEEEEEECCCCEEEEEECCCEEEEEEEEEECCCCEEEEECCCCHHHHCCC
NTILVDDGLIEMEVVSTTETEVKCKVLNNGDLGENKGVNLPGVSVKLPALSEKDKADLKF
CEEEEECCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHC
GCEQGVDFVAASFIRKEEDVKEIRALLVANGGEHIQIISKIENQEGVDNFDSILEASDGI
CHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPIEAVTIMATICARTDAVLKAELSSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
GAVDTAEKLSAPVIVVATEAGKSARSIRKYFPTARIIAVTTNTKTAAQLCLSKGITPMVV
CCCCCHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEEEE
DAIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL
EECCCCCEEEEECCEEEEECCCCCCCCEEEEEECEECCCCCCCCEEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESVEKLTELANAGMNVMRLNFSHGDFQEHGNRIDNLRQVMKNTGKQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHHHHHHHCCCCE
LAILLDTKGPEIRTIKLDNGNDVALVAGQDFTFTTDVSVVGNKDVVAVTYPGFAKDLVTG
EEEEEECCCCEEEEEEECCCCEEEEEECCCEEEEEEEEEECCCCEEEEECCCCHHHHCCC
NTILVDDGLIEMEVVSTTETEVKCKVLNNGDLGENKGVNLPGVSVKLPALSEKDKADLKF
CEEEEECCEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHC
GCEQGVDFVAASFIRKEEDVKEIRALLVANGGEHIQIISKIENQEGVDNFDSILEASDGI
CHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPIEAVTIMATICARTDAVLKAELSSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
GAVDTAEKLSAPVIVVATEAGKSARSIRKYFPTARIIAVTTNTKTAAQLCLSKGITPMVV
CCCCCHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCEEEE
DAIDSTDAFYLRGKELALESGIGAKGDIVVMVSGALVPSGTTNTASVHVL
EECCCCCEEEEECCEEEEECCCCCCCCEEEEEECEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]