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The map label for this gene is hldE

Identifier: 209696084

GI number: 209696084

Start: 2895086

End: 2896516

Strand: Reverse

Name: hldE

Synonym: VSAL_I2676

Alternate gene names: 209696084

Gene position: 2896516-2895086 (Counterclockwise)

Preceding gene: 209696085

Following gene: 209696082

Centisome position: 87.11

GC content: 43.19

Gene sequence:

>1431_bases
ATGAAACCGACACTTCCGAATTATGATCAATCTGGTGTATTGATTGTAGGTGATGTGATGCTTGACCGTTACTGGGTTGG
TCCTACAAGTCGTATCTCACCAGAAGCACCCGTTCCTGTCGTTAAAGTTGAAAATAATGAAGAACGTCCAGGTGGCGCAG
CCAACGTAGCAATGAACGTTGCAGCGTTAGGCGGACAAGCACATTTGGTTGGCTTAGTTGGTGAAGATGAACCAGCGCAA
GCATTAACAACAACGTTAGAATCACTAAAAGTACATTGTGATTTTGTTGCGTTACCTAATTTCCCGACGATTACTAAATT
ACGAGTGATGAGTCGTGGTCAGCAATTGATTCGTTTGGATTTTGAAGAAAGTTTCCATGATATTCCTGCTGAACCGATCA
TTAGCCGTATGCAAGCGACGTTACCCAAAGTAAAAGCCGTTGTATTATCTGATTATGCAAAAGGTGCTTTAGAGCATGTG
CAGCTGATGATCCAAGAAGCGCGTAAAGCGGGTGTACCTGTATTTATCGATCCAAAAGGCGCTGATTTTGAACGTTACCG
TGGTGCGACATTATTAACGCCTAATATGCTTGAATTTGAAACGGTTGTTGGCAAAGTAAAAGATGAAGAAGATTTAGTTG
TTAAAGGCCAACAAATCATCGAAGAGTTTGACTTTGAAGCCTTACTTGTTACACGCAGTGAGCATGGAATGACACTGCTT
CGTAGTGATATGGCGCCATTGCATTTACCGACGCAAGCTCGTGAAGTTTTTGATGTTACTGGTGCGGGTGATACGGTTAT
TTCGGTTTTAGCAGCATCAGTCTCAACTGGGAAACCTCTTGATGAAGCGTGTGCGTTAGCAAATGCCGCAGCAGGCGTTG
TTGTCGGTAAGTTAGGTACATCAACACTATCAACGATTGAATTAGCTGAAGCGATTCATGGTAGCCAAGATTCTGGTTTT
GGTATTATTAATGAAGAGCTACTGATCAGTGCGGTAAAACAAGCGCGTGAGCGTGGCGAAAAAGTCGTAATGACGAATGG
TTGTTTTGATATTCTACACGCAGGTCATGTGTATTATTTAAATCATGCAGCAGAGCTAGGCGATCGCTTGATCGTGGCTG
TGAATACGAATGAATCAGTACAACGCCTAAAAGGCCCTGGTCGTCCTATTAATCCGACGGATCGCCGTATGGCGGTACTT
GCAGGTTTAGGTGCTGTTGATTGGGTTGTTCCCTTCTCTGAAGACACACCACAACGTTTAATCTCTGAAGTATTGCCTAC
TTTATTAGTTAAAGGTGGCGATTATGAGATTAAAGACATTGCTGGTGGTGCTGAAGTGATTGCCGCAGGCGGTGAAGTGA
AAGTATTAAATTTTGAAGATGGTTGTTCAACAACGGGAATCATTGAAGCCATTAAAGGTGGTAGAGGCTAA

Upstream 100 bases:

>100_bases
CGCAGAACTTGCGTAAATGCTACCATTTATAAAGATATTATTTTTGATAGATAGCAACTGTGAGTAAACAGGTGATTGCT
ATTTACTGCCGGAGAAAAAA

Downstream 100 bases:

>100_bases
TTTAGTTTTTACTCTTTAATCGCTAATAAAAAAGCCACATTATAAATAATGTGGCTTTTTTTATTTGATATCAGGTTATT
TAGCTTGAGTTGTTTTACCC

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKPTLPNYDQSGVLIVGDVMLDRYWVGPTSRISPEAPVPVVKVENNEERPGGAANVAMNVAALGGQAHLVGLVGEDEPAQ
ALTTTLESLKVHCDFVALPNFPTITKLRVMSRGQQLIRLDFEESFHDIPAEPIISRMQATLPKVKAVVLSDYAKGALEHV
QLMIQEARKAGVPVFIDPKGADFERYRGATLLTPNMLEFETVVGKVKDEEDLVVKGQQIIEEFDFEALLVTRSEHGMTLL
RSDMAPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDSGF
GIINEELLISAVKQARERGEKVVMTNGCFDILHAGHVYYLNHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISEVLPTLLVKGGDYEIKDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG

Sequences:

>Translated_476_residues
MKPTLPNYDQSGVLIVGDVMLDRYWVGPTSRISPEAPVPVVKVENNEERPGGAANVAMNVAALGGQAHLVGLVGEDEPAQ
ALTTTLESLKVHCDFVALPNFPTITKLRVMSRGQQLIRLDFEESFHDIPAEPIISRMQATLPKVKAVVLSDYAKGALEHV
QLMIQEARKAGVPVFIDPKGADFERYRGATLLTPNMLEFETVVGKVKDEEDLVVKGQQIIEEFDFEALLVTRSEHGMTLL
RSDMAPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDSGF
GIINEELLISAVKQARERGEKVVMTNGCFDILHAGHVYYLNHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISEVLPTLLVKGGDYEIKDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG
>Mature_476_residues
MKPTLPNYDQSGVLIVGDVMLDRYWVGPTSRISPEAPVPVVKVENNEERPGGAANVAMNVAALGGQAHLVGLVGEDEPAQ
ALTTTLESLKVHCDFVALPNFPTITKLRVMSRGQQLIRLDFEESFHDIPAEPIISRMQATLPKVKAVVLSDYAKGALEHV
QLMIQEARKAGVPVFIDPKGADFERYRGATLLTPNMLEFETVVGKVKDEEDLVVKGQQIIEEFDFEALLVTRSEHGMTLL
RSDMAPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDSGF
GIINEELLISAVKQARERGEKVVMTNGCFDILHAGHVYYLNHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISEVLPTLLVKGGDYEIKDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=472, Percent_Identity=66.9491525423729, Blast_Score=643, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_ALISL (B6ELZ7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264014.1
- ProteinModelPortal:   B6ELZ7
- GeneID:   6989305
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2676
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   PRK11316
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.- [C]

Molecular weight: Translated: 50892; Mature: 50892

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTLPNYDQSGVLIVGDVMLDRYWVGPTSRISPEAPVPVVKVENNEERPGGAANVAMNV
CCCCCCCCCCCCEEEEEEEEECCEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHEEEE
AALGGQAHLVGLVGEDEPAQALTTTLESLKVHCDFVALPNFPTITKLRVMSRGQQLIRLD
EEECCCEEEEEEECCCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHCCCEEEEEE
FEESFHDIPAEPIISRMQATLPKVKAVVLSDYAKGALEHVQLMIQEARKAGVPVFIDPKG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ADFERYRGATLLTPNMLEFETVVGKVKDEEDLVVKGQQIIEEFDFEALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCCCHHEEHHHHHHHHCCCEEEEEEECCCCCHHH
RSDMAPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGT
HCCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCC
STLSTIELAEAIHGSQDSGFGIINEELLISAVKQARERGEKVVMTNGCFDILHAGHVYYL
CHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCEEEE
NHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVLAGLGAVDWVVPFSEDTPQRL
CCHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
ISEVLPTLLVKGGDYEIKDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG
HHHHHHHHEECCCCEEEHCCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKPTLPNYDQSGVLIVGDVMLDRYWVGPTSRISPEAPVPVVKVENNEERPGGAANVAMNV
CCCCCCCCCCCCEEEEEEEEECCEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHEEEE
AALGGQAHLVGLVGEDEPAQALTTTLESLKVHCDFVALPNFPTITKLRVMSRGQQLIRLD
EEECCCEEEEEEECCCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHCCCEEEEEE
FEESFHDIPAEPIISRMQATLPKVKAVVLSDYAKGALEHVQLMIQEARKAGVPVFIDPKG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ADFERYRGATLLTPNMLEFETVVGKVKDEEDLVVKGQQIIEEFDFEALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCCCHHEEHHHHHHHHCCCEEEEEEECCCCCHHH
RSDMAPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGT
HCCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCC
STLSTIELAEAIHGSQDSGFGIINEELLISAVKQARERGEKVVMTNGCFDILHAGHVYYL
CHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCEEEE
NHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVLAGLGAVDWVVPFSEDTPQRL
CCHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
ISEVLPTLLVKGGDYEIKDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG
HHHHHHHHEECCCCEEEHCCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA