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The map label for this gene is nudF [H]

Identifier: 209696082

GI number: 209696082

Start: 2892805

End: 2893452

Strand: Reverse

Name: nudF [H]

Synonym: VSAL_I2674

Alternate gene names: 209696082

Gene position: 2893452-2892805 (Counterclockwise)

Preceding gene: 209696084

Following gene: 209696081

Centisome position: 87.02

GC content: 39.97

Gene sequence:

>648_bases
ATGAATTCATCATCAAGTATAGTACGAGCGAATCAATTTACGGTTGATGATGTTGATGTTGTATCGAAAAATTCGTTATA
CAACGGTTTTTTTAAAATGACCAATATTGCTTTTCGTCATCGGTTATTCAGTGGTGGGTGGAGCGATATTATAGAGCGTG
AATTATTTGAACGCGGTCATGCCGTTGCTTTGTTGCCTTATGATCCGGTGACTGACAGTGTTGTTATTATCGAACAAATT
AGAGTTGGGGCTTTAGAGTCTGCTTCTCTATGGCAATATGAAATCGTTGCGGGCATGATTGATAAAGATGAGTCTGCAGA
ACAAGTTGCGATTAGAGAAACAGAAGAAGAAGCGGGGATTTCGGTCTCTAAATTAGAGAAAATATCACACTTTTACCCTT
CGTCGGGTGGGTGTACTGAACAGTTAGATGTGTTTGTTGGTTGCATTAACTCATTAGAAGCCATCGGTATACATGGCCTC
GAAGAGGAAGGTGAAGACATCAAGGTTCATGTTATGACTCGAGAAGATGCGTATGCTTTAGTGACTCGTGGCATAATTGA
GAATGCCGCCACAATTATTGCGCTCCAATGGTTAGAATTAAATGTTTCTCGCTTAAGATCACAGTGGAACATGATAGAAA
ATGCGTAA

Upstream 100 bases:

>100_bases
GATCTAAAAAAAATGTAAGCATTATCAAAAAAAATGAGTTAAAAAATTGTTGCTCTGTATTATGAGTGATAAAACAGAGT
TGAAATATTGGGAAATAAAA

Downstream 100 bases:

>100_bases
ATATCACGTTAATTTATCAGAGTTAATGCGAGTATATGAAACCAATTATGCCAAAATGGTGTCACTACTACCTAAGCCAG
AAGTGATTGGTGCTCTTGTT

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNSSSSIVRANQFTVDDVDVVSKNSLYNGFFKMTNIAFRHRLFSGGWSDIIERELFERGHAVALLPYDPVTDSVVIIEQI
RVGALESASLWQYEIVAGMIDKDESAEQVAIRETEEEAGISVSKLEKISHFYPSSGGCTEQLDVFVGCINSLEAIGIHGL
EEEGEDIKVHVMTREDAYALVTRGIIENAATIIALQWLELNVSRLRSQWNMIENA

Sequences:

>Translated_215_residues
MNSSSSIVRANQFTVDDVDVVSKNSLYNGFFKMTNIAFRHRLFSGGWSDIIERELFERGHAVALLPYDPVTDSVVIIEQI
RVGALESASLWQYEIVAGMIDKDESAEQVAIRETEEEAGISVSKLEKISHFYPSSGGCTEQLDVFVGCINSLEAIGIHGL
EEEGEDIKVHVMTREDAYALVTRGIIENAATIIALQWLELNVSRLRSQWNMIENA
>Mature_215_residues
MNSSSSIVRANQFTVDDVDVVSKNSLYNGFFKMTNIAFRHRLFSGGWSDIIERELFERGHAVALLPYDPVTDSVVIIEQI
RVGALESASLWQYEIVAGMIDKDESAEQVAIRETEEEAGISVSKLEKISHFYPSSGGCTEQLDVFVGCINSLEAIGIHGL
EEEGEDIKVHVMTREDAYALVTRGIIENAATIIALQWLELNVSRLRSQWNMIENA

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=198, Percent_Identity=49.4949494949495, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI1788810, Length=189, Percent_Identity=29.1005291005291, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23984; Mature: 23984

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSSSIVRANQFTVDDVDVVSKNSLYNGFFKMTNIAFRHRLFSGGWSDIIERELFERGH
CCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
AVALLPYDPVTDSVVIIEQIRVGALESASLWQYEIVAGMIDKDESAEQVAIRETEEEAGI
EEEEEECCCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHCC
SVSKLEKISHFYPSSGGCTEQLDVFVGCINSLEAIGIHGLEEEGEDIKVHVMTREDAYAL
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHH
VTRGIIENAATIIALQWLELNVSRLRSQWNMIENA
HHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSSSSIVRANQFTVDDVDVVSKNSLYNGFFKMTNIAFRHRLFSGGWSDIIERELFERGH
CCCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
AVALLPYDPVTDSVVIIEQIRVGALESASLWQYEIVAGMIDKDESAEQVAIRETEEEAGI
EEEEEECCCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHCC
SVSKLEKISHFYPSSGGCTEQLDVFVGCINSLEAIGIHGLEEEGEDIKVHVMTREDAYAL
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHH
VTRGIIENAATIIALQWLELNVSRLRSQWNMIENA
HHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]