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The map label for this gene is gloB

Identifier: 209695826

GI number: 209695826

Start: 2570113

End: 2570871

Strand: Direct

Name: gloB

Synonym: VSAL_I2401

Alternate gene names: 209695826

Gene position: 2570113-2570871 (Clockwise)

Preceding gene: 209695824

Following gene: 209695827

Centisome position: 77.29

GC content: 39.79

Gene sequence:

>759_bases
ATGCTATTAATAAAAAGCATACCTGCATTTAATGATAATTACATTTGGTTAATTCACAACAATGATAATCATTGTGTTGT
GGTTGATCCAGGAGAAGCAGCCCCAGTGCTTGCCTGCCTTAAAGAGCATGGTCTTATTTTAGATGCAATTTTAATTACGC
ACCACCACCATGATCATATTGGTGGGGTTCCTGAACTTGTTCGTCAATTTCCAAACATCAATGTTGTAGGCCCTGAAAAT
GAACCTATTCCAACCCTCACTCATCCCGTTGGTGATGGTGATTTTGTTGAATTATTCAATGAAAAATTCATGGTGCTAGG
CGTTGAAGGACACACAAAAGGTCATATCGCTTACATTGGTGATGAAAAACTATTTTGTGGTGATACCCTTTTCTCAGCCG
GTTGTGGTCGCTTATTTGAAGGCACCGCAGAACAAATGTTTCATTCACTGCAAAAATTGGCCGCATTACCCGATGAAACA
GAAGTGTATTGCGCCCATGAATACACTGCGTCCAATCTTGCTTTTGCGTTGGCGGTTGAACCTGATAACGATTATTTACA
ACAATATCGTGAAAAAGTTTTACGACTTCGAGCAAATGGAAAAGCAACGATCCCAACAACGATGCAACGAGAAAAGCTCA
TTAATCCTTTTTTAAGAACCAGTGAAGCAAGTGTAAAACAAGCCGTTTCGTCCAAAGTAGAAAATGATTCCGAAGTTGCA
ACCTTTGCCGCATTAAGGAGATGGAAAGACGAGTTTTAA

Upstream 100 bases:

>100_bases
ATGAATGCGGTTTTTCAATTTTTTTATCAAGCAGTGCTGGCTTAATCAATACAGTTACCTACAAAATATTAATACTTTAT
AAAAATTTGGAGAAAGGTGA

Downstream 100 bases:

>100_bases
TGATGAAACTTGTCTGGACGATTGTTGCCAAGTATTATCTGCACCCAAATAAAAAATAATCAAACACAACTTGAAGGCAA
TAATTAATGAAACTTAAGCT

Product: metallo-beta-lactamase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLLIKSIPAFNDNYIWLIHNNDNHCVVVDPGEAAPVLACLKEHGLILDAILITHHHHDHIGGVPELVRQFPNINVVGPEN
EPIPTLTHPVGDGDFVELFNEKFMVLGVEGHTKGHIAYIGDEKLFCGDTLFSAGCGRLFEGTAEQMFHSLQKLAALPDET
EVYCAHEYTASNLAFALAVEPDNDYLQQYREKVLRLRANGKATIPTTMQREKLINPFLRTSEASVKQAVSSKVENDSEVA
TFAALRRWKDEF

Sequences:

>Translated_252_residues
MLLIKSIPAFNDNYIWLIHNNDNHCVVVDPGEAAPVLACLKEHGLILDAILITHHHHDHIGGVPELVRQFPNINVVGPEN
EPIPTLTHPVGDGDFVELFNEKFMVLGVEGHTKGHIAYIGDEKLFCGDTLFSAGCGRLFEGTAEQMFHSLQKLAALPDET
EVYCAHEYTASNLAFALAVEPDNDYLQQYREKVLRLRANGKATIPTTMQREKLINPFLRTSEASVKQAVSSKVENDSEVA
TFAALRRWKDEF
>Mature_252_residues
MLLIKSIPAFNDNYIWLIHNNDNHCVVVDPGEAAPVLACLKEHGLILDAILITHHHHDHIGGVPELVRQFPNINVVGPEN
EPIPTLTHPVGDGDFVELFNEKFMVLGVEGHTKGHIAYIGDEKLFCGDTLFSAGCGRLFEGTAEQMFHSLQKLAALPDET
EVYCAHEYTASNLAFALAVEPDNDYLQQYREKVLRLRANGKATIPTTMQREKLINPFLRTSEASVKQAVSSKVENDSEVA
TFAALRRWKDEF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=257, Percent_Identity=38.1322957198444, Blast_Score=166, Evalue=1e-41,
Organism=Homo sapiens, GI21703352, Length=257, Percent_Identity=38.1322957198444, Blast_Score=166, Evalue=1e-41,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=36.9230769230769, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=36.9230769230769, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI14150041, Length=241, Percent_Identity=38.5892116182573, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI46361987, Length=192, Percent_Identity=41.1458333333333, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI1786406, Length=249, Percent_Identity=47.3895582329317, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17536925, Length=264, Percent_Identity=35.2272727272727, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324614, Length=265, Percent_Identity=29.0566037735849, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6320478, Length=263, Percent_Identity=28.5171102661597, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356335, Length=259, Percent_Identity=40.1544401544402, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=40.1544401544402, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24667711, Length=259, Percent_Identity=40.1544401544402, Blast_Score=178, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_ALISL (B6EJV4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263756.1
- GeneID:   6987738
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2401
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK874727
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28114; Mature: 28114

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLIKSIPAFNDNYIWLIHNNDNHCVVVDPGEAAPVLACLKEHGLILDAILITHHHHDHI
CEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEEEEHHHHCCCCCCC
GGVPELVRQFPNINVVGPENEPIPTLTHPVGDGDFVELFNEKFMVLGVEGHTKGHIAYIG
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCEEEEEC
DEKLFCGDTLFSAGCGRLFEGTAEQMFHSLQKLAALPDETEVYCAHEYTASNLAFALAVE
CCEEEECCHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEC
PDNDYLQQYREKVLRLRANGKATIPTTMQREKLINPFLRTSEASVKQAVSSKVENDSEVA
CCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
TFAALRRWKDEF
HHHHHHHHHCCC
>Mature Secondary Structure
MLLIKSIPAFNDNYIWLIHNNDNHCVVVDPGEAAPVLACLKEHGLILDAILITHHHHDHI
CEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEEEEHHHHCCCCCCC
GGVPELVRQFPNINVVGPENEPIPTLTHPVGDGDFVELFNEKFMVLGVEGHTKGHIAYIG
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCEEEEEC
DEKLFCGDTLFSAGCGRLFEGTAEQMFHSLQKLAALPDETEVYCAHEYTASNLAFALAVE
CCEEEECCHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEC
PDNDYLQQYREKVLRLRANGKATIPTTMQREKLINPFLRTSEASVKQAVSSKVENDSEVA
CCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
TFAALRRWKDEF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA