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The map label for this gene is rnhA

Identifier: 209695824

GI number: 209695824

Start: 2568848

End: 2569321

Strand: Direct

Name: rnhA

Synonym: VSAL_I2399

Alternate gene names: 209695824

Gene position: 2568848-2569321 (Clockwise)

Preceding gene: 209695820

Following gene: 209695826

Centisome position: 77.25

GC content: 44.3

Gene sequence:

>474_bases
ATGATAACAGAGATAATGAAACAGGTTGAAATTTTCACAGATGGCTCTTGCTTAGGCAATCCTGGTCCTGGTGGCTATGG
TATTGTTATGCGCTATAAAGGCACAGAAAAAACATTTTCAGGTGGGTTTAACCAAACCACAAACAACCGTATGGAAATGC
TTGCTGCTGTCGTCGCCTTACGTAACCTTAAAGAACCTTGTATTGTGGTGTTAACGACGGATAGCCAATACGTTCGTCAA
GGTATCACTCAATGGATACATGGTTGGAAAAAACGAGGGTGGAAAAAAGCAGATAAAAAACCAGTCGTAAATGCCGACCT
TTGGAAACAATTAGATGCAGAGGCAGAACGTCACACGGTCGATTGGCGCTGGGTTAAAGGTCATGCTGGTCATCGTGAGA
ATGAAATGTGTGACGATTTAGCACGTACCGCCGCAGAGAATCCAACCCAGGACGATACAGGTTACCCAGGCTAA

Upstream 100 bases:

>100_bases
CATACCGGTGGTTTCTGTATCGAGAACAATAATTCTATTCGGGGAATTGTCGCTAGTATTCATAGGCTCATTTGTGTCAG
ACTATTTCTAAATTCCTCTA

Downstream 100 bases:

>100_bases
TCTGAGTTCACTTTTCCCTGACGAGTCTGTGCTACCGAAATTGGAGCCAATTTTGGCTTCTTTTTCCAGGCCGATTTTAT
CGCTTTTAATGGATAGGTCC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSGGFNQTTNNRMEMLAAVVALRNLKEPCIVVLTTDSQYVRQ
GITQWIHGWKKRGWKKADKKPVVNADLWKQLDAEAERHTVDWRWVKGHAGHRENEMCDDLARTAAENPTQDDTGYPG

Sequences:

>Translated_157_residues
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSGGFNQTTNNRMEMLAAVVALRNLKEPCIVVLTTDSQYVRQ
GITQWIHGWKKRGWKKADKKPVVNADLWKQLDAEAERHTVDWRWVKGHAGHRENEMCDDLARTAAENPTQDDTGYPG
>Mature_157_residues
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSGGFNQTTNNRMEMLAAVVALRNLKEPCIVVLTTDSQYVRQ
GITQWIHGWKKRGWKKADKKPVVNADLWKQLDAEAERHTVDWRWVKGHAGHRENEMCDDLARTAAENPTQDDTGYPG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=34.965034965035, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=72.8476821192053, Blast_Score=244, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=34.0425531914894, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=36.8055555555556, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=36.8055555555556, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_ALISL (B6EJV2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263754.1
- ProteinModelPortal:   B6EJV2
- GeneID:   6988420
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2399
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17711; Mature: 17711

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSGGFNQTTNNRMEMLAAVVAL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
RNLKEPCIVVLTTDSQYVRQGITQWIHGWKKRGWKKADKKPVVNADLWKQLDAEAERHTV
HCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DWRWVKGHAGHRENEMCDDLARTAAENPTQDDTGYPG
CEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MITEIMKQVEIFTDGSCLGNPGPGGYGIVMRYKGTEKTFSGGFNQTTNNRMEMLAAVVAL
CHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
RNLKEPCIVVLTTDSQYVRQGITQWIHGWKKRGWKKADKKPVVNADLWKQLDAEAERHTV
HCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DWRWVKGHAGHRENEMCDDLARTAAENPTQDDTGYPG
CEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA