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The map label for this gene is mltD [H]

Identifier: 209695827

GI number: 209695827

Start: 2570958

End: 2572511

Strand: Direct

Name: mltD [H]

Synonym: VSAL_I2402

Alternate gene names: 209695827

Gene position: 2570958-2572511 (Clockwise)

Preceding gene: 209695826

Following gene: 209695852

Centisome position: 77.32

GC content: 40.15

Gene sequence:

>1554_bases
ATGAAACTTAAGCTCCATTGGATTGGATTAGCGTTATTGGCAGGCTGTCAAAGTGCTCCACCATCAGAAAAAACACAGCC
TGATACGACTTCTGCTATCTCTACGCCTTCAAAGCAAGATACCAAACCAGCAGAAATTAAATCAATCGAAAAACCGGAGC
TTACCCCTCAAGAACAAGAAGACGTATGGCAACGTATTGCAATGCAATTAAATATGCCAATTCCAGATCATAAATCGGTC
GATTATTATCGTAATTGGTACATTAAACACCCAAGTCACCTGCGTACTGTTGCCAAACGAGCTCAACCGTTTCTGTATAT
GATCACTGTTGAAATTGAAAAACGCGGACTTCCTATGGAGTTAGTTCTTCTTCCTATCGTTGAAAGTGCGTTTGACCCTT
TTGCTTACTCCCACGGCAGTGCCGCTGGTCTATGGCAATTTGTCCCAGGAACGGCTGATCGCTTTAAATTAGAACGTAAT
TGGTGGTATGACGGTCGTCGTGATGTACCTGCGGCAACGAATGCAGCATTAGATTACATGGAATATTTTGACCGTCGCTT
TGATGGCAATTGGCAACAAGCGATTGCATCTTACAACAGTGGTGGTGGACGAGTATCTAGAGCAATCCGAAAAAATAAAA
ACTTAGGTAAACCTACCGATTTCTTTTCATTAGATTTACCAGAAGAAACCAGCGGTTATGTCCCTAAATTATTAGCATTA
GCTGACATCATTAAAAACCATGAAAAATACGGGGTAGAATTGCCACCTATTGCCAACAAACCTGTACTCGAAATGGTAGA
CCCAAATAAACAATTAGATTTGGCGATTGCGGCAGAATTTGCGGGTATTTCAATCTCTGAATTGCAAGGCTATAACCCTG
GATACAATCAATGGGCAACGTCACCAGATGGCCCTTATCATCTATTATTACCGATTGATAAAGTCGATCGTTTTAATGCC
AATCTGAAAAAGAATGGCAATAAAACAATGAAAGTTGTGCGCTACAAAGTTCAATCCGGCGATACAATCAGTCAATTAGC
AGTGAAGCATAATACGACCTCTAGCATCATTGAACAAACCAATGACTTAAATGGTACGAGCATTCGAGTTGATCAATATT
TATTAATTCCAACGGCTTCTCAAGGCAATAAAACCTATGCATTAAGTGCACCACAGCGCTTAAAGAAAACTCAAGCCAAA
GCAAAAGGAAAATACAAATCTGACCATACCGTTAAAGATGGAGAATCGTTATGGAGCATCGCTCGTGATAACAGCATCTC
TTATCGTGATTTAGCAAAATGGAACGGCATGGCACCAAAAGATTCATTGCGTGTTGGACAAAAATTAGTCATTTGGAAAA
GCAATTCTGATGGTGGGATAATCCGTACGGTTCATTACCAAATCCGTCAAGGCGATAACCTTAGCTCTATTGCACAAAAA
TTCAGTGTCTCTGTGACAGACATCATGAAATGGAATAGCATCGAACGTGGCTCTTATATTAAGCCGGGACAAAAATTAAA
ACTTTACTTTGATGTTACTAAGGCAAATGTATGA

Upstream 100 bases:

>100_bases
AAGACGAGTTTTAATGATGAAACTTGTCTGGACGATTGTTGCCAAGTATTATCTGCACCCAAATAAAAAATAATCAAACA
CAACTTGAAGGCAATAATTA

Downstream 100 bases:

>100_bases
CCCCTTCTTCAAATCCGTTAATTTTATTAGTCGATATTTTTCGATCGCCAACAGATTGTTTCGCCGCGTTATATGAACAT
GGAAAATGGGCTTGGCTTCC

Product: membrane-bound lytic murein transglycosylase D precursor

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKLKLHWIGLALLAGCQSAPPSEKTQPDTTSAISTPSKQDTKPAEIKSIEKPELTPQEQEDVWQRIAMQLNMPIPDHKSV
DYYRNWYIKHPSHLRTVAKRAQPFLYMITVEIEKRGLPMELVLLPIVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERN
WWYDGRRDVPAATNAALDYMEYFDRRFDGNWQQAIASYNSGGGRVSRAIRKNKNLGKPTDFFSLDLPEETSGYVPKLLAL
ADIIKNHEKYGVELPPIANKPVLEMVDPNKQLDLAIAAEFAGISISELQGYNPGYNQWATSPDGPYHLLLPIDKVDRFNA
NLKKNGNKTMKVVRYKVQSGDTISQLAVKHNTTSSIIEQTNDLNGTSIRVDQYLLIPTASQGNKTYALSAPQRLKKTQAK
AKGKYKSDHTVKDGESLWSIARDNSISYRDLAKWNGMAPKDSLRVGQKLVIWKSNSDGGIIRTVHYQIRQGDNLSSIAQK
FSVSVTDIMKWNSIERGSYIKPGQKLKLYFDVTKANV

Sequences:

>Translated_517_residues
MKLKLHWIGLALLAGCQSAPPSEKTQPDTTSAISTPSKQDTKPAEIKSIEKPELTPQEQEDVWQRIAMQLNMPIPDHKSV
DYYRNWYIKHPSHLRTVAKRAQPFLYMITVEIEKRGLPMELVLLPIVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERN
WWYDGRRDVPAATNAALDYMEYFDRRFDGNWQQAIASYNSGGGRVSRAIRKNKNLGKPTDFFSLDLPEETSGYVPKLLAL
ADIIKNHEKYGVELPPIANKPVLEMVDPNKQLDLAIAAEFAGISISELQGYNPGYNQWATSPDGPYHLLLPIDKVDRFNA
NLKKNGNKTMKVVRYKVQSGDTISQLAVKHNTTSSIIEQTNDLNGTSIRVDQYLLIPTASQGNKTYALSAPQRLKKTQAK
AKGKYKSDHTVKDGESLWSIARDNSISYRDLAKWNGMAPKDSLRVGQKLVIWKSNSDGGIIRTVHYQIRQGDNLSSIAQK
FSVSVTDIMKWNSIERGSYIKPGQKLKLYFDVTKANV
>Mature_517_residues
MKLKLHWIGLALLAGCQSAPPSEKTQPDTTSAISTPSKQDTKPAEIKSIEKPELTPQEQEDVWQRIAMQLNMPIPDHKSV
DYYRNWYIKHPSHLRTVAKRAQPFLYMITVEIEKRGLPMELVLLPIVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERN
WWYDGRRDVPAATNAALDYMEYFDRRFDGNWQQAIASYNSGGGRVSRAIRKNKNLGKPTDFFSLDLPEETSGYVPKLLAL
ADIIKNHEKYGVELPPIANKPVLEMVDPNKQLDLAIAAEFAGISISELQGYNPGYNQWATSPDGPYHLLLPIDKVDRFNA
NLKKNGNKTMKVVRYKVQSGDTISQLAVKHNTTSSIIEQTNDLNGTSIRVDQYLLIPTASQGNKTYALSAPQRLKKTQAK
AKGKYKSDHTVKDGESLWSIARDNSISYRDLAKWNGMAPKDSLRVGQKLVIWKSNSDGGIIRTVHYQIRQGDNLSSIAQK
FSVSVTDIMKWNSIERGSYIKPGQKLKLYFDVTKANV

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=468, Percent_Identity=35.4700854700855, Blast_Score=281, Evalue=7e-77,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58232; Mature: 58232

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLHWIGLALLAGCQSAPPSEKTQPDTTSAISTPSKQDTKPAEIKSIEKPELTPQEQE
CEEEEEEHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCCCCCHHH
DVWQRIAMQLNMPIPDHKSVDYYRNWYIKHPSHLRTVAKRAQPFLYMITVEIEKRGLPME
HHHHHHHHHCCCCCCCCCCCCHHHCEEEECHHHHHHHHHHCCCEEEEEEEEEECCCCCHH
LVLLPIVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERNWWYDGRRDVPAATNAALDYM
HHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEECCCCCCCCCCCCCHHHHHHHHH
EYFDRRFDGNWQQAIASYNSGGGRVSRAIRKNKNLGKPTDFFSLDLPEETSGYVPKLLAL
HHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHH
ADIIKNHEKYGVELPPIANKPVLEMVDPNKQLDLAIAAEFAGISISELQGYNPGYNQWAT
HHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEEEHHCCCCHHHHCCCCCCCCCCCC
SPDGPYHLLLPIDKVDRFNANLKKNGNKTMKVVRYKVQSGDTISQLAVKHNTTSSIIEQT
CCCCCEEEEEECCHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHH
NDLNGTSIRVDQYLLIPTASQGNKTYALSAPQRLKKTQAKAKGKYKSDHTVKDGESLWSI
CCCCCCEEEEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
ARDNSISYRDLAKWNGMAPKDSLRVGQKLVIWKSNSDGGIIRTVHYQIRQGDNLSSIAQK
HCCCCCCHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHH
FSVSVTDIMKWNSIERGSYIKPGQKLKLYFDVTKANV
HCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MKLKLHWIGLALLAGCQSAPPSEKTQPDTTSAISTPSKQDTKPAEIKSIEKPELTPQEQE
CEEEEEEHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCCCCCHHH
DVWQRIAMQLNMPIPDHKSVDYYRNWYIKHPSHLRTVAKRAQPFLYMITVEIEKRGLPME
HHHHHHHHHCCCCCCCCCCCCHHHCEEEECHHHHHHHHHHCCCEEEEEEEEEECCCCCHH
LVLLPIVESAFDPFAYSHGSAAGLWQFVPGTADRFKLERNWWYDGRRDVPAATNAALDYM
HHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEECCCCCCCCCCCCCHHHHHHHHH
EYFDRRFDGNWQQAIASYNSGGGRVSRAIRKNKNLGKPTDFFSLDLPEETSGYVPKLLAL
HHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHH
ADIIKNHEKYGVELPPIANKPVLEMVDPNKQLDLAIAAEFAGISISELQGYNPGYNQWAT
HHHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEEEEEEHHCCCCHHHHCCCCCCCCCCCC
SPDGPYHLLLPIDKVDRFNANLKKNGNKTMKVVRYKVQSGDTISQLAVKHNTTSSIIEQT
CCCCCEEEEEECCHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHH
NDLNGTSIRVDQYLLIPTASQGNKTYALSAPQRLKKTQAKAKGKYKSDHTVKDGESLWSI
CCCCCCEEEEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
ARDNSISYRDLAKWNGMAPKDSLRVGQKLVIWKSNSDGGIIRTVHYQIRQGDNLSSIAQK
HCCCCCCHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHH
FSVSVTDIMKWNSIERGSYIKPGQKLKLYFDVTKANV
HCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]