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The map label for this gene is narQ [H]

Identifier: 209695794

GI number: 209695794

Start: 2542267

End: 2543958

Strand: Direct

Name: narQ [H]

Synonym: VSAL_I2369

Alternate gene names: 209695794

Gene position: 2542267-2543958 (Clockwise)

Preceding gene: 209695789

Following gene: 209695795

Centisome position: 76.46

GC content: 37.06

Gene sequence:

>1692_bases
ATGAAACATAACAAAACATCAATATTAACAAAAACAATAGCTCAAGTGATGTTGTGGATTGTCCTTATTTCTATCATTAC
AACAAGCTTAGCTTTGGTCACATTATCATCAAGTTTAAAAGACGCCGAAGCGGTCAATATTGCCGGTTCTTTACGCATGC
AAAGTTATCGTTTGGCCTACGATATTGAAACCAATTCACCATACATGCTAGATCACTTAGCCAAGTTTTCAGAATCCATT
GAATCACCCGCTTTCCACTCTTTAGATCAATGGTTTGTACCGGACGACATTGAATCCTATTATTCTGACATTCGATTACA
GTGGCAGTTATTGCAACCTGCATTACTAAGTGATGATAAACAAATTTATCTCACTAAAGTGTCGCTCTTTGTGGATGAAA
TCGATCATTTCGTTCTACGCTTACAGCAATTTTCAGAGCGAAAACTTCAATTGCTTTCTTTTATTGGTGCGATAGGTTTA
TCTCTCATTCTTTGTCTTGGCCTTTTTATTATTTCATTTACTCAACGAAAAATTGTGAGTCCGCTGCATCATCTTGTTGC
CGCAAGTCACTCAATGACAAAAGGCAATTTTTCAGTACAAGTGGATTTAAATAGTGGTAATGAATTAGGGCAATTAGGCA
GCGCCTTTAATCACATGGCAAAACAAGTGCACTCTAGCTACCGCGAGCTCGAAAGTCATGTTGAAGATAAAACCAGAAAA
CTCAGTCAAGCAAATCGATCGCTAACCACTCTTTATGCTTGCTCTCAAGAGCTATCAGCGTCTCAACTTGATGAAAAAGC
CTTTAAAAACATTCTTAACATGTTTACTAATATTGAAGGTGTTATTAGTGCAAGGTTAATTGTAGAAGAAGAGTCTGGGG
GTGATTGGGAAATAACAAGTGGAACCCCAAACAACTCACCTTGGAGTTTAGAAGAGCTGATCATTGATGGAGAAAAATTA
GGTTACTTATTGTGGCAATTTTCTCTTCCATGCCCAGACCAAAAGCTAATCATCAATATCGCAAATATTCTTGCTCGTGG
GTTGTTCTATGACTTAACCCAAAAACAAACGCATCAACTTATTCTTCTTGAAGAACGAGCAGCAATTGCACGAGAACTGC
ATGATTCACTGGCACAATCGCTTTCTTATTTAAAAATTCAAACGACCTTATTGAAACGCCAACTTAAGAAATGTGATTGT
CAAAACACGGGGAGTACATTGACGGAATTGGATGAGGGACTTAAAAGTGCCTATACTCAGTTAAGAGAATTATTAAACAC
CTTTAGATTAACGATTAATAAGGCGCACTTTGGTGAAGCATTACAAGAGATTATAAATACCTTACAATCGCAGACAGCCA
TTCGTATACATGTAAATAATGAACTGCCATCTTTACCAATTAATGCACAACAGCACGTACATTTGTTGCAATTAATACGC
GAAGCAACATTAAATGCCATCAAACACTCAAAAGCTGGTAATATTATCATTACGTGTTACCAAGAAGGTGAGCAAGGTTA
CATTAACATTGAAGATGATGGCGTAGGATTTGATTCCACCGAAGAAAAAATAAATCATTATGGATTAAGAATAATGCAAG
AACGAGCAAACTTCGTTCGAGGTACTTTATCCATTGCATCAGAATTAGAACACGGTTGTGCAGTGAACGTTACGTTTCCA
CTTAACCAATAA

Upstream 100 bases:

>100_bases
ACAAGGGGGGAACTTGTTCTAGATCAATTTTTAAAACAAATAAAAACAACAGAATAGATTATTATCCCCTCCTTTTAACT
ATAGGCTAGCCACAACAATT

Downstream 100 bases:

>100_bases
TTAATTTAAAAGGTATATACCCTATGTGGAATGTTGTTATCGTTGACGATCATCCTTTAATGCGCCGAGGCATTGGCCAA
CTTCTTTCTTTTGAAGATGA

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MKHNKTSILTKTIAQVMLWIVLISIITTSLALVTLSSSLKDAEAVNIAGSLRMQSYRLAYDIETNSPYMLDHLAKFSESI
ESPAFHSLDQWFVPDDIESYYSDIRLQWQLLQPALLSDDKQIYLTKVSLFVDEIDHFVLRLQQFSERKLQLLSFIGAIGL
SLILCLGLFIISFTQRKIVSPLHHLVAASHSMTKGNFSVQVDLNSGNELGQLGSAFNHMAKQVHSSYRELESHVEDKTRK
LSQANRSLTTLYACSQELSASQLDEKAFKNILNMFTNIEGVISARLIVEEESGGDWEITSGTPNNSPWSLEELIIDGEKL
GYLLWQFSLPCPDQKLIINIANILARGLFYDLTQKQTHQLILLEERAAIARELHDSLAQSLSYLKIQTTLLKRQLKKCDC
QNTGSTLTELDEGLKSAYTQLRELLNTFRLTINKAHFGEALQEIINTLQSQTAIRIHVNNELPSLPINAQQHVHLLQLIR
EATLNAIKHSKAGNIIITCYQEGEQGYINIEDDGVGFDSTEEKINHYGLRIMQERANFVRGTLSIASELEHGCAVNVTFP
LNQ

Sequences:

>Translated_563_residues
MKHNKTSILTKTIAQVMLWIVLISIITTSLALVTLSSSLKDAEAVNIAGSLRMQSYRLAYDIETNSPYMLDHLAKFSESI
ESPAFHSLDQWFVPDDIESYYSDIRLQWQLLQPALLSDDKQIYLTKVSLFVDEIDHFVLRLQQFSERKLQLLSFIGAIGL
SLILCLGLFIISFTQRKIVSPLHHLVAASHSMTKGNFSVQVDLNSGNELGQLGSAFNHMAKQVHSSYRELESHVEDKTRK
LSQANRSLTTLYACSQELSASQLDEKAFKNILNMFTNIEGVISARLIVEEESGGDWEITSGTPNNSPWSLEELIIDGEKL
GYLLWQFSLPCPDQKLIINIANILARGLFYDLTQKQTHQLILLEERAAIARELHDSLAQSLSYLKIQTTLLKRQLKKCDC
QNTGSTLTELDEGLKSAYTQLRELLNTFRLTINKAHFGEALQEIINTLQSQTAIRIHVNNELPSLPINAQQHVHLLQLIR
EATLNAIKHSKAGNIIITCYQEGEQGYINIEDDGVGFDSTEEKINHYGLRIMQERANFVRGTLSIASELEHGCAVNVTFP
LNQ
>Mature_563_residues
MKHNKTSILTKTIAQVMLWIVLISIITTSLALVTLSSSLKDAEAVNIAGSLRMQSYRLAYDIETNSPYMLDHLAKFSESI
ESPAFHSLDQWFVPDDIESYYSDIRLQWQLLQPALLSDDKQIYLTKVSLFVDEIDHFVLRLQQFSERKLQLLSFIGAIGL
SLILCLGLFIISFTQRKIVSPLHHLVAASHSMTKGNFSVQVDLNSGNELGQLGSAFNHMAKQVHSSYRELESHVEDKTRK
LSQANRSLTTLYACSQELSASQLDEKAFKNILNMFTNIEGVISARLIVEEESGGDWEITSGTPNNSPWSLEELIIDGEKL
GYLLWQFSLPCPDQKLIINIANILARGLFYDLTQKQTHQLILLEERAAIARELHDSLAQSLSYLKIQTTLLKRQLKKCDC
QNTGSTLTELDEGLKSAYTQLRELLNTFRLTINKAHFGEALQEIINTLQSQTAIRIHVNNELPSLPINAQQHVHLLQLIR
EATLNAIKHSKAGNIIITCYQEGEQGYINIEDDGVGFDSTEEKINHYGLRIMQERANFVRGTLSIASELEHGCAVNVTFP
LNQ

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=557, Percent_Identity=41.4721723518851, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1787474, Length=579, Percent_Identity=27.8065630397237, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87082311, Length=197, Percent_Identity=24.8730964467005, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63456; Mature: 63456

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHNKTSILTKTIAQVMLWIVLISIITTSLALVTLSSSLKDAEAVNIAGSLRMQSYRLAY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEE
DIETNSPYMLDHLAKFSESIESPAFHSLDQWFVPDDIESYYSDIRLQWQLLQPALLSDDK
EECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHEEHHHHHCHHHHCCCC
QIYLTKVSLFVDEIDHFVLRLQQFSERKLQLLSFIGAIGLSLILCLGLFIISFTQRKIVS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLHHLVAASHSMTKGNFSVQVDLNSGNELGQLGSAFNHMAKQVHSSYRELESHVEDKTRK
HHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQANRSLTTLYACSQELSASQLDEKAFKNILNMFTNIEGVISARLIVEEESGGDWEITS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEEEEECCCCCEEEEC
GTPNNSPWSLEELIIDGEKLGYLLWQFSLPCPDQKLIINIANILARGLFYDLTQKQTHQL
CCCCCCCCCHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ILLEERAAIARELHDSLAQSLSYLKIQTTLLKRQLKKCDCQNTGSTLTELDEGLKSAYTQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LRELLNTFRLTINKAHFGEALQEIINTLQSQTAIRIHVNNELPSLPINAQQHVHLLQLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EATLNAIKHSKAGNIIITCYQEGEQGYINIEDDGVGFDSTEEKINHYGLRIMQERANFVR
HHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GTLSIASELEHGCAVNVTFPLNQ
HHHHHHHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MKHNKTSILTKTIAQVMLWIVLISIITTSLALVTLSSSLKDAEAVNIAGSLRMQSYRLAY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEEEE
DIETNSPYMLDHLAKFSESIESPAFHSLDQWFVPDDIESYYSDIRLQWQLLQPALLSDDK
EECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHEEHHHHHCHHHHCCCC
QIYLTKVSLFVDEIDHFVLRLQQFSERKLQLLSFIGAIGLSLILCLGLFIISFTQRKIVS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLHHLVAASHSMTKGNFSVQVDLNSGNELGQLGSAFNHMAKQVHSSYRELESHVEDKTRK
HHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQANRSLTTLYACSQELSASQLDEKAFKNILNMFTNIEGVISARLIVEEESGGDWEITS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEEEEECCCCCEEEEC
GTPNNSPWSLEELIIDGEKLGYLLWQFSLPCPDQKLIINIANILARGLFYDLTQKQTHQL
CCCCCCCCCHHHHHHCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
ILLEERAAIARELHDSLAQSLSYLKIQTTLLKRQLKKCDCQNTGSTLTELDEGLKSAYTQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LRELLNTFRLTINKAHFGEALQEIINTLQSQTAIRIHVNNELPSLPINAQQHVHLLQLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
EATLNAIKHSKAGNIIITCYQEGEQGYINIEDDGVGFDSTEEKINHYGLRIMQERANFVR
HHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GTLSIASELEHGCAVNVTFPLNQ
HHHHHHHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]