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The map label for this gene is trpE [H]

Identifier: 209694703

GI number: 209694703

Start: 1254941

End: 1256578

Strand: Reverse

Name: trpE [H]

Synonym: VSAL_I1154

Alternate gene names: 209694703

Gene position: 1256578-1254941 (Counterclockwise)

Preceding gene: 209694706

Following gene: 209694702

Centisome position: 37.79

GC content: 40.42

Gene sequence:

>1638_bases
ATGCCAATACAAAAGAAATTAACAGAAGATCAACGAATTAGCAGAGAGGCTATGATGCAATCATCACACTCAAAAGATGG
TCAGTTAGAATTACTTTGCCTTGAGGTACCCTACGTTTCCGACCCAACGGAATTGTATTCGACACTGTGTGCAAACCAAG
CCATGAGCTTGTTACTTGAGTCTGCTGAAGTTGATTCAAAACAAAACCTAAAAAGCTTAATGTTGATTGATGCAGCAGTC
CGTATTCATTGTTTCGATAAAATCGTTACTCTTGAAGCATTAACGAAAAACGGAGAAGCATTATTAAAAACATTACGTCT
TTCTCTTCCTTCTTCGTTAGTTGATTTGATTTCCAGCTCGGTACTTAAGCTTCATTTTCCAGAGATTAATCAAGATTTAG
ATGAAGACAGTCGCCTAAGAGAGCTTTCTTCTTTTGATGCATTACGCTTTATCCAAAACCAATTCGATTTGACCGGATTA
CCGTCAAATGCACTCTTTTTGGGTGGTTTATTTGCTTATGACTTAGTGGCAAATTTTGAACAACTAGGCAGTGCAGAAAC
AACCAACAAATGTCCTGATTACCTATTTTATATTGCAGAATCTTTACTCGTCATAGACCATCAAACAAAAACATCGACAG
TACAAGGCTCTATATTTACAAAAGAGCCAGCAATCAAACAAAACATATCGACTCGTTTAGAAGCTATTAATGTTTTTTGT
TCAGAACCTCACACCTTGCCTGATGCTCCAATATTAGATCGTTGTGAAGCTCTTCCAAGTGTCTCTGATGAAGATTTTTG
TCAAACGGTTCTGGATTTAAAAGAATACGTTATTAGAGGCGACGTATTTCAGGTGGTTCCATCTCGTCGCTTTACGCTTC
CTTGCCCTGCGCCGCTTTCTGCTTACCGAGAGTTAAAAATCGGCAACCCAAGTCCTTACATGTTCTACCTGCAAGATCGT
GATTTTACTTTATTTGGGGCTTCTCCTGAGTCTGCGTTAAAATATTGTAAAGACAGCAATCAAGTCGAAATTTACCCAAT
AGCAGGGACTCGCCAGCGTGGGAAAAATAACGATGGTTCAATTAATTATGACTTAGATAGCCGTATTGAATTGGAATTAC
GTTGTGATACCAAAGAAAACGCAGAACACATGATGTTGGTGGATCTTGCTCGAAATGACGTAGCTCGAATTTCAGCCCCT
GGTACAAGATACGTTGCTGATTTATTAAAAGTCGATCGCTACAGCCACGTAATGCATTTAGTTTCTCGTGTTGTTGGACA
GCTACGAGGGGATCTTGATGCCCTTCATGCTTATCAAGCGTGTATGAACATGGGGACCTTAACAGGCGCCCCTAAAATTC
GAGCAATGCAATTAATCCGTGATGTAGAAAAAACACGTCGTGGAACTTATGGTGGTGCCGTTGGTTATTTAACGGGTGAT
GGCCACATGGATACTTGTATCGTTATTCGCTCTGCTTATGTAGAAGATGGCATAGCTCAAGTTCAAGCCGGTGCAGGGGT
CGTTTATGATTCTCATCCACAAGCTGAAGCAGATGAAACACGAGGTAAAGCTCAAGCCGTTATCTCTGCAATTCAAAAGG
CGCACCTTGCATCACAGAAAGCAAAGGAGAAAAACTAA

Upstream 100 bases:

>100_bases
TAAAAGAGAAAATACAACATCGATGAAATAGTAAACATTCAGTAATTTAGCCCTCATAAGTTTTAACAAAAGAGTGGCAA
CAACAGTGAAATATGCGACT

Downstream 100 bases:

>100_bases
TGACTCATATCGTATTACTGGATAATTTTGACTCCTTTACTTACAACCTTGTCGATCAGTTTCGCTCACTTGGTTACCCT
GTAACTATATATCGAAACAG

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MPIQKKLTEDQRISREAMMQSSHSKDGQLELLCLEVPYVSDPTELYSTLCANQAMSLLLESAEVDSKQNLKSLMLIDAAV
RIHCFDKIVTLEALTKNGEALLKTLRLSLPSSLVDLISSSVLKLHFPEINQDLDEDSRLRELSSFDALRFIQNQFDLTGL
PSNALFLGGLFAYDLVANFEQLGSAETTNKCPDYLFYIAESLLVIDHQTKTSTVQGSIFTKEPAIKQNISTRLEAINVFC
SEPHTLPDAPILDRCEALPSVSDEDFCQTVLDLKEYVIRGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFYLQDR
DFTLFGASPESALKYCKDSNQVEIYPIAGTRQRGKNNDGSINYDLDSRIELELRCDTKENAEHMMLVDLARNDVARISAP
GTRYVADLLKVDRYSHVMHLVSRVVGQLRGDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGTYGGAVGYLTGD
GHMDTCIVIRSAYVEDGIAQVQAGAGVVYDSHPQAEADETRGKAQAVISAIQKAHLASQKAKEKN

Sequences:

>Translated_545_residues
MPIQKKLTEDQRISREAMMQSSHSKDGQLELLCLEVPYVSDPTELYSTLCANQAMSLLLESAEVDSKQNLKSLMLIDAAV
RIHCFDKIVTLEALTKNGEALLKTLRLSLPSSLVDLISSSVLKLHFPEINQDLDEDSRLRELSSFDALRFIQNQFDLTGL
PSNALFLGGLFAYDLVANFEQLGSAETTNKCPDYLFYIAESLLVIDHQTKTSTVQGSIFTKEPAIKQNISTRLEAINVFC
SEPHTLPDAPILDRCEALPSVSDEDFCQTVLDLKEYVIRGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFYLQDR
DFTLFGASPESALKYCKDSNQVEIYPIAGTRQRGKNNDGSINYDLDSRIELELRCDTKENAEHMMLVDLARNDVARISAP
GTRYVADLLKVDRYSHVMHLVSRVVGQLRGDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGTYGGAVGYLTGD
GHMDTCIVIRSAYVEDGIAQVQAGAGVVYDSHPQAEADETRGKAQAVISAIQKAHLASQKAKEKN
>Mature_544_residues
PIQKKLTEDQRISREAMMQSSHSKDGQLELLCLEVPYVSDPTELYSTLCANQAMSLLLESAEVDSKQNLKSLMLIDAAVR
IHCFDKIVTLEALTKNGEALLKTLRLSLPSSLVDLISSSVLKLHFPEINQDLDEDSRLRELSSFDALRFIQNQFDLTGLP
SNALFLGGLFAYDLVANFEQLGSAETTNKCPDYLFYIAESLLVIDHQTKTSTVQGSIFTKEPAIKQNISTRLEAINVFCS
EPHTLPDAPILDRCEALPSVSDEDFCQTVLDLKEYVIRGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFYLQDRD
FTLFGASPESALKYCKDSNQVEIYPIAGTRQRGKNNDGSINYDLDSRIELELRCDTKENAEHMMLVDLARNDVARISAPG
TRYVADLLKVDRYSHVMHLVSRVVGQLRGDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGTYGGAVGYLTGDG
HMDTCIVIRSAYVEDGIAQVQAGAGVVYDSHPQAEADETRGKAQAVISAIQKAHLASQKAKEKN

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=515, Percent_Identity=58.252427184466, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1788114, Length=370, Percent_Identity=30.8108108108108, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1786809, Length=215, Percent_Identity=29.7674418604651, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=406, Percent_Identity=35.9605911330049, Blast_Score=230, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324361, Length=392, Percent_Identity=25.2551020408163, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 60532; Mature: 60400

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIQKKLTEDQRISREAMMQSSHSKDGQLELLCLEVPYVSDPTELYSTLCANQAMSLLLE
CCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
SAEVDSKQNLKSLMLIDAAVRIHCFDKIVTLEALTKNGEALLKTLRLSLPSSLVDLISSS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
VLKLHFPEINQDLDEDSRLRELSSFDALRFIQNQFDLTGLPSNALFLGGLFAYDLVANFE
HEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGSAETTNKCPDYLFYIAESLLVIDHQTKTSTVQGSIFTKEPAIKQNISTRLEAINVFC
HHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEECCCEECCCCHHHHHHHHHHHHHHHHH
SEPHTLPDAPILDRCEALPSVSDEDFCQTVLDLKEYVIRGDVFQVVPSRRFTLPCPAPLS
CCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHEECCCCCEECCCCCCHH
AYRELKIGNPSPYMFYLQDRDFTLFGASPESALKYCKDSNQVEIYPIAGTRQRGKNNDGS
HHHEEECCCCCCEEEEEECCCEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
INYDLDSRIELELRCDTKENAEHMMLVDLARNDVARISAPGTRYVADLLKVDRYSHVMHL
EEECCCCEEEEEEEECCCCCCCEEEEEEEHHCCHHEECCCCHHHHHHHHHHHHHHHHHHH
VSRVVGQLRGDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGTYGGAVGYLTGD
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GHMDTCIVIRSAYVEDGIAQVQAGAGVVYDSHPQAEADETRGKAQAVISAIQKAHLASQK
CCCCHHEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKEKN
HCCCC
>Mature Secondary Structure 
PIQKKLTEDQRISREAMMQSSHSKDGQLELLCLEVPYVSDPTELYSTLCANQAMSLLLE
CCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
SAEVDSKQNLKSLMLIDAAVRIHCFDKIVTLEALTKNGEALLKTLRLSLPSSLVDLISSS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
VLKLHFPEINQDLDEDSRLRELSSFDALRFIQNQFDLTGLPSNALFLGGLFAYDLVANFE
HEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGSAETTNKCPDYLFYIAESLLVIDHQTKTSTVQGSIFTKEPAIKQNISTRLEAINVFC
HHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEECCCEECCCCHHHHHHHHHHHHHHHHH
SEPHTLPDAPILDRCEALPSVSDEDFCQTVLDLKEYVIRGDVFQVVPSRRFTLPCPAPLS
CCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHEECCCCCEECCCCCCHH
AYRELKIGNPSPYMFYLQDRDFTLFGASPESALKYCKDSNQVEIYPIAGTRQRGKNNDGS
HHHEEECCCCCCEEEEEECCCEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
INYDLDSRIELELRCDTKENAEHMMLVDLARNDVARISAPGTRYVADLLKVDRYSHVMHL
EEECCCCEEEEEEEECCCCCCCEEEEEEEHHCCHHEECCCCHHHHHHHHHHHHHHHHHHH
VSRVVGQLRGDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGTYGGAVGYLTGD
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECC
GHMDTCIVIRSAYVEDGIAQVQAGAGVVYDSHPQAEADETRGKAQAVISAIQKAHLASQK
CCCCHHEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKEKN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]