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The map label for this gene is trpG [H]

Identifier: 209694702

GI number: 209694702

Start: 1254333

End: 1254941

Strand: Reverse

Name: trpG [H]

Synonym: VSAL_I1153

Alternate gene names: 209694702

Gene position: 1254941-1254333 (Counterclockwise)

Preceding gene: 209694703

Following gene: 209694701

Centisome position: 37.74

GC content: 41.87

Gene sequence:

>609_bases
ATGACTCATATCGTATTACTGGATAATTTTGACTCCTTTACTTACAACCTTGTCGATCAGTTTCGCTCACTTGGTTACCC
TGTAACTATATATCGAAACAGTTTATCTGCGATAGCATTTGAAGACGCGGTCACTAAATTAGAAAACCCTGTTGTTGTAT
TATCCCCGGGGCCTGGTGCCCCATCAGAAGCAGGTTGCATGCCTGACGTTATTCAGCGCTTAAAAGGCAAAGTTCCGATG
ATAGGGATTTGTCTTGGTCATCAAGCCATCGTTGAAGCGTACGACGGCATTGTCGCTGGTGCGGGTGAAATTGTTCATGG
TAAATCAGCCATGATGACACATAACCAACACGCCATTTTTGATGGATTACCAAATCCACTTTCTATTGCTCGTTACCACT
CTCTTGTTGCCACTAAAGTTACCAAGCAATTAACAGTAACGGCAACGGTTGACGATTTAGTTATGGCGGTCATTCAAGAA
GAAGATAAAGTGTGTGGGTTTCAGTTTCATCCCGAATCCATTCTCACTACGCAAGGCGAACAACTTTTAATTAATACCTT
GAATTGGGCACTCGATATCACTCAATCCCATTCAGAAAATAAAGAATAA

Upstream 100 bases:

>100_bases
CACAAGCTGAAGCAGATGAAACACGAGGTAAAGCTCAAGCCGTTATCTCTGCAATTCAAAAGGCGCACCTTGCATCACAG
AAAGCAAAGGAGAAAAACTA

Downstream 100 bases:

>100_bases
GGATATTCTCATGACTCAAATTGAAGCCATTTTTAATAAGCTATACAGCCAAGTTTCTCTTTCAGAAACTGAGAGCCAAA
TTCTATTTGATGCCATCATC

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLSAIAFEDAVTKLENPVVVLSPGPGAPSEAGCMPDVIQRLKGKVPM
IGICLGHQAIVEAYDGIVAGAGEIVHGKSAMMTHNQHAIFDGLPNPLSIARYHSLVATKVTKQLTVTATVDDLVMAVIQE
EDKVCGFQFHPESILTTQGEQLLINTLNWALDITQSHSENKE

Sequences:

>Translated_202_residues
MTHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLSAIAFEDAVTKLENPVVVLSPGPGAPSEAGCMPDVIQRLKGKVPM
IGICLGHQAIVEAYDGIVAGAGEIVHGKSAMMTHNQHAIFDGLPNPLSIARYHSLVATKVTKQLTVTATVDDLVMAVIQE
EDKVCGFQFHPESILTTQGEQLLINTLNWALDITQSHSENKE
>Mature_201_residues
THIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLSAIAFEDAVTKLENPVVVLSPGPGAPSEAGCMPDVIQRLKGKVPMI
GICLGHQAIVEAYDGIVAGAGEIVHGKSAMMTHNQHAIFDGLPNPLSIARYHSLVATKVTKQLTVTATVDDLVMAVIQEE
DKVCGFQFHPESILTTQGEQLLINTLNWALDITQSHSENKE

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=190, Percent_Identity=60, Blast_Score=247, Evalue=3e-67,
Organism=Escherichia coli, GI1789760, Length=188, Percent_Identity=44.1489361702128, Blast_Score=156, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6322638, Length=195, Percent_Identity=41.025641025641, Blast_Score=147, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6324361, Length=209, Percent_Identity=28.2296650717703, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 21987; Mature: 21856

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLSAIAFEDAVTKLENPVVVLSPGPGA
CEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCC
PSEAGCMPDVIQRLKGKVPMIGICLGHQAIVEAYDGIVAGAGEIVHGKSAMMTHNQHAIF
CCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHCCEEECCCCEEECCCCEEECCCCCEE
DGLPNPLSIARYHSLVATKVTKQLTVTATVDDLVMAVIQEEDKVCGFQFHPESILTTQGE
CCCCCHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCCCCCCCEEECCCHHHHCCCC
QLLINTLNWALDITQSHSENKE
EEEEEEHHHEEEECCCCCCCCC
>Mature Secondary Structure 
THIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLSAIAFEDAVTKLENPVVVLSPGPGA
EEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCC
PSEAGCMPDVIQRLKGKVPMIGICLGHQAIVEAYDGIVAGAGEIVHGKSAMMTHNQHAIF
CCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHCCEEECCCCEEECCCCEEECCCCCEE
DGLPNPLSIARYHSLVATKVTKQLTVTATVDDLVMAVIQEEDKVCGFQFHPESILTTQGE
CCCCCHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCCCCCCCEEECCCHHHHCCCC
QLLINTLNWALDITQSHSENKE
EEEEEEHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]