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The map label for this gene is trpB [H]

Identifier: 209694699

GI number: 209694699

Start: 1250696

End: 1251886

Strand: Reverse

Name: trpB [H]

Synonym: VSAL_I1150

Alternate gene names: 209694699

Gene position: 1251886-1250696 (Counterclockwise)

Preceding gene: 209694700

Following gene: 209694698

Centisome position: 37.65

GC content: 44.75

Gene sequence:

>1191_bases
ATGGCAAAATTAGATGCCTACTTCGGCGAATATGGTGGTCAATACGTACCACAAATTTTAGTTCCTGCACTTGATCAACT
TGAGCAAGCCTTTATTGATGCTCAAGCAGACGAAGATTTCCGTGCCGAGTTCATGACACTATTGCAAGAATATGCGGGTC
GCCCTACCGCTCTTACCCTTTGTCGTAACCTAACTAAGGGTACTAAAACTAAGTTATACCTAAAACGTGAAGATTTACTG
CACGGTGGCGCGCACAAAACCAATCAAGTATTGGGTCAAGCACTGCTCGCTTTACGTATGGGTAAAAAAGAAATCATCGC
TGAAACAGGTGCCGGTCAACACGGTGTAGCAACGGCTCTTGCCTGCGCATTGCTTGGCTTAAAATGCCGCGTTTACATGG
GTGCTAAAGATATTGAACGTCAAAGCCCGAATGTATTCCGCATGGAATTAATGGGCGCAGAAGTGATCCCCGTTCATTCA
GGTTCAGCAACACTAAAAGATGCCTGTAATGAAGCATTGCGTGATTGGTCTGCAACGTATGAAACTGCCCACTACCTATT
GGGCACTGCTGCGGGTCCTCACCCATTCCCAACCATCGTTCGCGAATTCCAACGCATGATTGGCGAAGAAACTAAAATGC
AAATCCTTGCTCGTGAAGGTCGCCTTCCTGATGCCGTAATTGCTTGTGTCGGTGGCGGCTCAAATGCAATAGGTATGTTT
GCGGATTTCATCAAAGAAGAAAATGTAAGACTGATTGGTGTCGAACCCGCAGGTAAAGGCATTGATACCGACCAACACGG
GGCACCATTAAAACATGGAAAAACCGGTATTTTCTTTGGTATGAAAGCGCCATTAATGCAAGATGAACACGGTCAAATTG
AAGAATCTTATTCTGTTTCTGCGGGTCTTGATTTTCCATCAGTTGGTCCACAACACGCTCACTTGAATTCGATTGGCCGT
GCTGAATACGGCTCAGTAACCGATGATGAAGCACTCGAAGCATTTCAAATATTAGCAAAAGGCGAAGGCATTATTCCGGC
ACTGGAGTCTTCTCATGCGCTTGCTTATGCATTACAAATGGCACATTCAGAGCCAGAAAAAGAACAACTATTAGTGGTAA
ACCTATCTGGTCGTGGTGATAAAGACATTTTCACCGTTCATGACATCCTAAAAGAAAAGGGAGAAATCTAA

Upstream 100 bases:

>100_bases
CTCGATTTCAACTCTGGAGTAGAAACGACTCCAGGGAAAAAAGATCATAAAAAATTAAGCGCCGTATTTAGCGCATTACG
AAATTATTAAGGAAAATATC

Downstream 100 bases:

>100_bases
TGGATCGTTATCAAGCCCTATTTGCTAAATTAGCAGAAAAAAACCAAGGTGCATTTGTTCCTTTTGTAACCATTGGCGAT
CCAAATCCAGAACTTTCTTA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADEDFRAEFMTLLQEYAGRPTALTLCRNLTKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLALRMGKKEIIAETGAGQHGVATALACALLGLKCRVYMGAKDIERQSPNVFRMELMGAEVIPVHS
GSATLKDACNEALRDWSATYETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
ADFIKEENVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVSAGLDFPSVGPQHAHLNSIGR
AEYGSVTDDEALEAFQILAKGEGIIPALESSHALAYALQMAHSEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI

Sequences:

>Translated_396_residues
MAKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADEDFRAEFMTLLQEYAGRPTALTLCRNLTKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLALRMGKKEIIAETGAGQHGVATALACALLGLKCRVYMGAKDIERQSPNVFRMELMGAEVIPVHS
GSATLKDACNEALRDWSATYETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
ADFIKEENVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVSAGLDFPSVGPQHAHLNSIGR
AEYGSVTDDEALEAFQILAKGEGIIPALESSHALAYALQMAHSEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI
>Mature_395_residues
AKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADEDFRAEFMTLLQEYAGRPTALTLCRNLTKGTKTKLYLKREDLLH
GGAHKTNQVLGQALLALRMGKKEIIAETGAGQHGVATALACALLGLKCRVYMGAKDIERQSPNVFRMELMGAEVIPVHSG
SATLKDACNEALRDWSATYETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMFA
DFIKEENVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVSAGLDFPSVGPQHAHLNSIGRA
EYGSVTDDEALEAFQILAKGEGIIPALESSHALAYALQMAHSEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=393, Percent_Identity=81.9338422391858, Blast_Score=673, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=389, Percent_Identity=51.9280205655527, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 42928; Mature: 42796

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADEDFRAEFMTLLQEYAGRPTALTL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
CRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQALLALRMGKKEIIAETGAGQHGVATAL
HHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHH
ACALLGLKCRVYMGAKDIERQSPNVFRMELMGAEVIPVHSGSATLKDACNEALRDWSATY
HHHHHHHHEEEEECCHHHHCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
ETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIKEENVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVS
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAHLNSIGRAEYGSVTDDEALEAFQILAKGEGIIPALESSHALAYALQM
CCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
AHSEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKLDAYFGEYGGQYVPQILVPALDQLEQAFIDAQADEDFRAEFMTLLQEYAGRPTALTL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
CRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQALLALRMGKKEIIAETGAGQHGVATAL
HHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHH
ACALLGLKCRVYMGAKDIERQSPNVFRMELMGAEVIPVHSGSATLKDACNEALRDWSATY
HHHHHHHHEEEEECCHHHHCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
ETAHYLLGTAAGPHPFPTIVREFQRMIGEETKMQILAREGRLPDAVIACVGGGSNAIGMF
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIKEENVRLIGVEPAGKGIDTDQHGAPLKHGKTGIFFGMKAPLMQDEHGQIEESYSVS
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAHLNSIGRAEYGSVTDDEALEAFQILAKGEGIIPALESSHALAYALQM
CCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
AHSEPEKEQLLVVNLSGRGDKDIFTVHDILKEKGEI
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]