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The map label for this gene is trpA

Identifier: 209694698

GI number: 209694698

Start: 1249890

End: 1250696

Strand: Reverse

Name: trpA

Synonym: VSAL_I1149

Alternate gene names: 209694698

Gene position: 1250696-1249890 (Counterclockwise)

Preceding gene: 209694699

Following gene: 209694697

Centisome position: 37.61

GC content: 41.02

Gene sequence:

>807_bases
ATGGATCGTTATCAAGCCCTATTTGCTAAATTAGCAGAAAAAAACCAAGGTGCATTTGTTCCTTTTGTAACCATTGGCGA
TCCAAATCCAGAACTTTCTTATGAAATCATGGATACTTTAGTAAAAGCAGGTGCCGATGCACTTGAACTTGGTATCCCAT
TCTCTGATCCTTTGGCTGATGGACCAACAATTCAAGGTGCAAATATTCGTGCATTAAACAGTAAAACAACGCCTATCGTA
TGTTTTGATTTAATCGCAAAAATCCGAGCTAAATATCCTGAAACCCCAATTGGCCTATTAGTTTACGCCAACCTTGTCTT
CGCAAATGGCATTGATACTTTCTATACTAAATGTCAGCAAGCTGGTGTAGATTCAGTGTTAATTGCAGACGTACCAACCA
ATGAATCAGAAGAGTTTAGAACAAGTGCAATTAAACACAATGTTCACCCTATCTTCATTGCGCCTCCGTCAGCCTCAGAT
GAAACATTAGAAACCGTTGCTAAGCTTGGTGGGGGTTATACTTACCTTCTTTCTCGTGCAGGTGTTACTGGTGCTGAAAC
TAAAGCGGGCATGCCTGTTGCTCAATTATTAGCACGTTTAAACCAATTTGATGCACCTCCTGCGATTCTTGGTTTTGGTA
TATCGGAACCTGCTCAAGTAGAAGAAGCGATTAAAGCCGGTGCAGCAGGCGCAATTTCAGGCTCTGCAACTGTTAAAATC
ATTGAACAACATCAAGACAATCCAGCGGCGTTACTTGATGCACTTACGGCATTTACTTCTGCCATGAAAGCAGCAACACA
AAAATAA

Upstream 100 bases:

>100_bases
AGCCAGAAAAAGAACAACTATTAGTGGTAAACCTATCTGGTCGTGGTGATAAAGACATTTTCACCGTTCATGACATCCTA
AAAGAAAAGGGAGAAATCTA

Downstream 100 bases:

>100_bases
CCAAAACACCTTATTCATTATTTAAAGGAGCTCATTTTGAGCTCCTTTTTTAATTCAATAATCCCTATTAAAAATAACTA
CGTTAATTATCAAAATGAAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MDRYQALFAKLAEKNQGAFVPFVTIGDPNPELSYEIMDTLVKAGADALELGIPFSDPLADGPTIQGANIRALNSKTTPIV
CFDLIAKIRAKYPETPIGLLVYANLVFANGIDTFYTKCQQAGVDSVLIADVPTNESEEFRTSAIKHNVHPIFIAPPSASD
ETLETVAKLGGGYTYLLSRAGVTGAETKAGMPVAQLLARLNQFDAPPAILGFGISEPAQVEEAIKAGAAGAISGSATVKI
IEQHQDNPAALLDALTAFTSAMKAATQK

Sequences:

>Translated_268_residues
MDRYQALFAKLAEKNQGAFVPFVTIGDPNPELSYEIMDTLVKAGADALELGIPFSDPLADGPTIQGANIRALNSKTTPIV
CFDLIAKIRAKYPETPIGLLVYANLVFANGIDTFYTKCQQAGVDSVLIADVPTNESEEFRTSAIKHNVHPIFIAPPSASD
ETLETVAKLGGGYTYLLSRAGVTGAETKAGMPVAQLLARLNQFDAPPAILGFGISEPAQVEEAIKAGAAGAISGSATVKI
IEQHQDNPAALLDALTAFTSAMKAATQK
>Mature_268_residues
MDRYQALFAKLAEKNQGAFVPFVTIGDPNPELSYEIMDTLVKAGADALELGIPFSDPLADGPTIQGANIRALNSKTTPIV
CFDLIAKIRAKYPETPIGLLVYANLVFANGIDTFYTKCQQAGVDSVLIADVPTNESEEFRTSAIKHNVHPIFIAPPSASD
ETLETVAKLGGGYTYLLSRAGVTGAETKAGMPVAQLLARLNQFDAPPAILGFGISEPAQVEEAIKAGAAGAISGSATVKI
IEQHQDNPAALLDALTAFTSAMKAATQK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=62.1722846441948, Blast_Score=330, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6321412, Length=241, Percent_Identity=30.2904564315353, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ALISL (B6EJA2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262626.1
- GeneID:   6986869
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1149
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28221; Mature: 28221

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRYQALFAKLAEKNQGAFVPFVTIGDPNPELSYEIMDTLVKAGADALELGIPFSDPLAD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQGANIRALNSKTTPIVCFDLIAKIRAKYPETPIGLLVYANLVFANGIDTFYTKCQQ
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AGVDSVLIADVPTNESEEFRTSAIKHNVHPIFIAPPSASDETLETVAKLGGGYTYLLSRA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHC
GVTGAETKAGMPVAQLLARLNQFDAPPAILGFGISEPAQVEEAIKAGAAGAISGSATVKI
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEE
IEQHQDNPAALLDALTAFTSAMKAATQK
EECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRYQALFAKLAEKNQGAFVPFVTIGDPNPELSYEIMDTLVKAGADALELGIPFSDPLAD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQGANIRALNSKTTPIVCFDLIAKIRAKYPETPIGLLVYANLVFANGIDTFYTKCQQ
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AGVDSVLIADVPTNESEEFRTSAIKHNVHPIFIAPPSASDETLETVAKLGGGYTYLLSRA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHC
GVTGAETKAGMPVAQLLARLNQFDAPPAILGFGISEPAQVEEAIKAGAAGAISGSATVKI
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEE
IEQHQDNPAALLDALTAFTSAMKAATQK
EECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA