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The map label for this gene is pdxB

Identifier: 209694631

GI number: 209694631

Start: 1164455

End: 1165585

Strand: Direct

Name: pdxB

Synonym: VSAL_I1072

Alternate gene names: 209694631

Gene position: 1164455-1165585 (Clockwise)

Preceding gene: 209694630

Following gene: 209694632

Centisome position: 35.02

GC content: 38.9

Gene sequence:

>1131_bases
ATGAAAATATTGATTGATGAGAATATGCCTTATGCCGCAGAACTCTTTAGTGAACTCGGCGAAGTAATAGCTAAATCAGG
AAGAACCTTGACGGCTGATGATTTAATTGACGTTGACGCGTTGATGATTCGTTCAGTCACTAAAGTGAATTCAGAATTAT
TGAGTAAAGCGAATAAACTGTCTTTTGTTGGTACTGCTACTGCTGGTATGGATCACGTTAACCAAGCTTTATTGAAAAAT
AAAGGTGTCTTTTTTACCGCGGCTCCAGGCTGTAATAAAGTAGGCGTTGCTGAATACGTATTAAGTTGTGTGATGGTATT
AGCTCAGCAACATGGTTTTTCTATTTTTGATAAAACGTTCGGTATTATTGGTGCGGGCCAAGTGGGTTCATATTTAGCTC
AATGTCTTGATGCGTTAAGTATTCCTTATCTATTGAATGATCCAATTAAAGAGCAAGAAGGGGACACTCGTTCTTTTGTT
TCTTTAGATGAATTATTAGAAAAGTCAGATGTTATTAGTCTCCATACCCCTATTACACGTGATGGTAATTTCCCTACACA
TCATTTGATTGGTCAAGAGCGTTTATTAACATTGCGCCATGACCAAATATTGATCAATGCCGCTCGTGGTCCCGTGGTTG
ATAATGATGCGCTAAAAAATCGCCTATTAATCCAAGATGGTTTTAAAGCCGTATTGGATGTTTTTGAGTTTGAACCAGAG
GTTGATATAGCGCTCTTGCCATTGCTTTCTTTTGCAACGCCGCACATTGCAGGTTATGGCTTGGAAGGTAAAGCTAGAGG
AACGACCATGATATTTAATTCGTTCTGTGAGTTCTTACAACGGGAAGAGCGTGCGGATCCTCAAAATCTATTGCCTGTTG
CTCCAATCCCTAATGTGACACTAAATCAGCAGTGGGATCATTCAACACTGCACAATTTGATTCAGTTAGTCTATGATGTG
CGAAAAGATGATGCGGTATTTAGAAAAGAAATCTCAACAATCGGTGCGTTTGATAATATGCGTAAAGATTATTGGGATAG
AAGAGAATACAGTGCAATCACTATCACGGGTAATAAAGACTGTGGTCTTACCCCGTTAAAACAACTTGGATTTACAATCG
AGGAAGTCTAA

Upstream 100 bases:

>100_bases
TATGACTGTTTTTTATTGCTAGGATTAATACTTAGGTAGATAGGTGTAATCGAGCGGTGATACACTGAACCGATGAATCT
AAAGGATAGATATTAAAAAT

Downstream 100 bases:

>100_bases
TGACTCAAGAATTTGATGTTGCCATTCTAGGCGCAACTGGTGCCGTTGGCGAAGCAATCATAAATGTATTACAAGAGCGC
AAGTTTCCTGTACGTAATTT

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKILIDENMPYAAELFSELGEVIAKSGRTLTADDLIDVDALMIRSVTKVNSELLSKANKLSFVGTATAGMDHVNQALLKN
KGVFFTAAPGCNKVGVAEYVLSCVMVLAQQHGFSIFDKTFGIIGAGQVGSYLAQCLDALSIPYLLNDPIKEQEGDTRSFV
SLDELLEKSDVISLHTPITRDGNFPTHHLIGQERLLTLRHDQILINAARGPVVDNDALKNRLLIQDGFKAVLDVFEFEPE
VDIALLPLLSFATPHIAGYGLEGKARGTTMIFNSFCEFLQREERADPQNLLPVAPIPNVTLNQQWDHSTLHNLIQLVYDV
RKDDAVFRKEISTIGAFDNMRKDYWDRREYSAITITGNKDCGLTPLKQLGFTIEEV

Sequences:

>Translated_376_residues
MKILIDENMPYAAELFSELGEVIAKSGRTLTADDLIDVDALMIRSVTKVNSELLSKANKLSFVGTATAGMDHVNQALLKN
KGVFFTAAPGCNKVGVAEYVLSCVMVLAQQHGFSIFDKTFGIIGAGQVGSYLAQCLDALSIPYLLNDPIKEQEGDTRSFV
SLDELLEKSDVISLHTPITRDGNFPTHHLIGQERLLTLRHDQILINAARGPVVDNDALKNRLLIQDGFKAVLDVFEFEPE
VDIALLPLLSFATPHIAGYGLEGKARGTTMIFNSFCEFLQREERADPQNLLPVAPIPNVTLNQQWDHSTLHNLIQLVYDV
RKDDAVFRKEISTIGAFDNMRKDYWDRREYSAITITGNKDCGLTPLKQLGFTIEEV
>Mature_376_residues
MKILIDENMPYAAELFSELGEVIAKSGRTLTADDLIDVDALMIRSVTKVNSELLSKANKLSFVGTATAGMDHVNQALLKN
KGVFFTAAPGCNKVGVAEYVLSCVMVLAQQHGFSIFDKTFGIIGAGQVGSYLAQCLDALSIPYLLNDPIKEQEGDTRSFV
SLDELLEKSDVISLHTPITRDGNFPTHHLIGQERLLTLRHDQILINAARGPVVDNDALKNRLLIQDGFKAVLDVFEFEPE
VDIALLPLLSFATPHIAGYGLEGKARGTTMIFNSFCEFLQREERADPQNLLPVAPIPNVTLNQQWDHSTLHNLIQLVYDV
RKDDAVFRKEISTIGAFDNMRKDYWDRREYSAITITGNKDCGLTPLKQLGFTIEEV

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=308, Percent_Identity=27.5974025974026, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1788660, Length=371, Percent_Identity=48.2479784366577, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI87082289, Length=266, Percent_Identity=30.0751879699248, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789279, Length=257, Percent_Identity=29.1828793774319, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787645, Length=210, Percent_Identity=24.7619047619048, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=266, Percent_Identity=28.1954887218045, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=384, Percent_Identity=26.0416666666667, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=385, Percent_Identity=26.7532467532467, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=161, Percent_Identity=29.1925465838509, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921140, Length=292, Percent_Identity=25.6849315068493, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_ALISL (B6EIW3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262559.1
- GeneID:   6988464
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1072
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- ProtClustDB:   CLSK874680
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 41682; Mature: 41682

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 209-209 ACT_SITE 238-238 ACT_SITE 255-255 BINDING 45-45 BINDING 67-67 BINDING 147-147 BINDING 176-176 BINDING 233-233 BINDING 258-258 BINDING 259-259

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIDENMPYAAELFSELGEVIAKSGRTLTADDLIDVDALMIRSVTKVNSELLSKANKL
CEEEEECCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SFVGTATAGMDHVNQALLKNKGVFFTAAPGCNKVGVAEYVLSCVMVLAQQHGFSIFDKTF
EEEEECHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
GIIGAGQVGSYLAQCLDALSIPYLLNDPIKEQEGDTRSFVSLDELLEKSDVISLHTPITR
CCEECCHHHHHHHHHHHHHCCCHHHCCCCHHCCCCHHHHHHHHHHHHHCCEEEEECCCCC
DGNFPTHHLIGQERLLTLRHDQILINAARGPVVDNDALKNRLLIQDGFKAVLDVFEFEPE
CCCCCHHHHCCHHHHEEEECCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCC
VDIALLPLLSFATPHIAGYGLEGKARGTTMIFNSFCEFLQREERADPQNLLPVAPIPNVT
CCHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
LNQQWDHSTLHNLIQLVYDVRKDDAVFRKEISTIGAFDNMRKDYWDRREYSAITITGNKD
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
CGLTPLKQLGFTIEEV
CCCCHHHHHCCCHHCC
>Mature Secondary Structure
MKILIDENMPYAAELFSELGEVIAKSGRTLTADDLIDVDALMIRSVTKVNSELLSKANKL
CEEEEECCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SFVGTATAGMDHVNQALLKNKGVFFTAAPGCNKVGVAEYVLSCVMVLAQQHGFSIFDKTF
EEEEECHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
GIIGAGQVGSYLAQCLDALSIPYLLNDPIKEQEGDTRSFVSLDELLEKSDVISLHTPITR
CCEECCHHHHHHHHHHHHHCCCHHHCCCCHHCCCCHHHHHHHHHHHHHCCEEEEECCCCC
DGNFPTHHLIGQERLLTLRHDQILINAARGPVVDNDALKNRLLIQDGFKAVLDVFEFEPE
CCCCCHHHHCCHHHHEEEECCEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCC
VDIALLPLLSFATPHIAGYGLEGKARGTTMIFNSFCEFLQREERADPQNLLPVAPIPNVT
CCHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCC
LNQQWDHSTLHNLIQLVYDVRKDDAVFRKEISTIGAFDNMRKDYWDRREYSAITITGNKD
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
CGLTPLKQLGFTIEEV
CCCCHHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA