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The map label for this gene is mrdA [H]

Identifier: 209694548

GI number: 209694548

Start: 1088013

End: 1089890

Strand: Reverse

Name: mrdA [H]

Synonym: VSAL_I0985

Alternate gene names: 209694548

Gene position: 1089890-1088013 (Counterclockwise)

Preceding gene: 209694549

Following gene: 209694547

Centisome position: 32.78

GC content: 41.05

Gene sequence:

>1878_bases
TTGTTTAAGCGCAGACGCACACAGATACGTGACTATCATGCAGAATCGAGATTATTTGTTTCTAGAGCAATAGTCTCTTT
TGTCGGGATATTGGTTCTCATTAGCCTCTTATTGACTAATTTATACAATATCCAAATCAATGAATTTCAAGATTATAAAA
CACGTTCAAACGATAACCGCATAAAAATCGTACCTATAGCCCCAAATCGAGGCCTTATTTATGATCGTAATCGTATGCCA
CTCGCGGAAAATCGTCCTGTTTTTAATTTAGAAATCACACCAGATAAAGTGAAAGATCTGTCTGCGCTATTTACTGAAAT
ACAGACTATCTTGCCTATTTCGCAAGATAATATTGATGACTTCACAAAAGAGCGTCGTCGTACTCGTCGTTTTAAATCCA
TACCAATTCTGTCTCAATTAACCCAAGAACAAGTGGCCATTTTCTCAGTAAACCAACACAAATTTTCTGGTGTAGAAATA
AAGGCTTATCTAAAACGCTTCTATCCTTATGGAAAAACGTTTACGCATGTACTGGGCTATGTTGCAAAAATTAATGATAG
AGATTTAGCTCGATTAGCTAGAGAAGAAAAAGATGCCAATTATCAAGCTACCCGAGATATAGGTAAGCTAGGTATCGAAC
GCTACTACGAAGATACCCTTCATGGTACGGCGGGTTATCAAGAAGTCGAGGTTAACAGTCGCGGTCGCGTAGTACGTACA
TTAAAATACGTCCCACCAATCCCAGGCAAAGACATTGTTTTAAATCTTGATATCGAAATTCAAAAATTTGTGGCTAAACA
ATTAGACGGCCGTCGTGGTGCTGCAATTGTATTAGAACCGAACACAAGTGCCGTATTAGCGATGGTATCAAGCCCTAGCT
ATGATCCAAATTTATTTGTACATGGCATTTCCAGTAAAAACTATAACGCATTATTGAATAATCCAGATCGCCCATTAGTT
AACCGAGCGACGCTGGGTATCTATCCACCAGCGTCAACGGTAAAACCTTTTATTGCCGTCTCTGCATTAGAAGAGAAGAT
AGTTACTCCGCTCACCACCCGCAATGATCCGGGCTACTGGCGTATTCCTAACTCAAAAACCAGACCATTCCGAGATTGGA
AACGTTGGGGTCATGGAACCGTGAATTTAAAACAAGCGATAGAAGAATCTGTTGATACTTTCTTCTACCAAATATCTTTT
GATATGGGAATTGACCGCTTATCAGCATGGATGATGAAATTTGGTTTTGGTGATTATACCGGCATCGATATCCATGAAGA
AAGCAAAGCAAATATGCCAACTCGAGATTGGAAAGTCGCTCGTTTCCGTACACCTTGGTATCAAGGCGATACGATTCCAG
TCGGTATTGGACAAGGTTATTGGACTGCAACACCGATGCAAATAGCTAAGGCGACTTCTGTGCTCGTGAATCGCGGTAAA
GTTCAAGCGCCTCACTTATTGAAAACAATTATTGATGGTAACGTTGAAACCCCGTTTGAACCAGAACCGTACCCTCCAAT
AGAAGGGATTAGAGACAGTTATTGGCAGCTCGCCATTGATGGCATGATCTTAGTGAATAATGGTGCTAAAGGTACAGCCA
GACGTGTGTTTGCTGGCACTCCTTATGTTAGTGCAGGTAAATCAGGCTCATCTCAAGTCTTTGGTTTAAATGGCCAAGAA
TACAACGCTGATGAGATTGCAGAACGTTTACGTGACCATGCTTTGTTTACTGGCTTTGCTCCAGCAAAAGATCCAAAAGT
CATTATCTCGCTCGTTCTTGAAAATGGCGGCGGCGGTTCTAGCCAAGGCGGCCCAATTGCACGAAGTGTATTTGACCATG
TGTTATTACCAGCAACAGGAGAGGTGAGTAATAAATAA

Upstream 100 bases:

>100_bases
ATTAACGCTTCCTCACCCTCTTGTTCGTGTAGTTATGGCTGAAAGCCTGTACCGAGCATGGAGTATTACGGCCAACCACC
CTTACCACAGAGAATAAGAG

Downstream 100 bases:

>100_bases
TGAGAGTTGATATCGCATCAGGAAAAAATCGTGACTTTTTCCATCGAATTCATATAGATTTGCCCTTACTGCTTGGCATC
TTATTATTAATGGCTTGTGC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MFKRRRTQIRDYHAESRLFVSRAIVSFVGILVLISLLLTNLYNIQINEFQDYKTRSNDNRIKIVPIAPNRGLIYDRNRMP
LAENRPVFNLEITPDKVKDLSALFTEIQTILPISQDNIDDFTKERRRTRRFKSIPILSQLTQEQVAIFSVNQHKFSGVEI
KAYLKRFYPYGKTFTHVLGYVAKINDRDLARLAREEKDANYQATRDIGKLGIERYYEDTLHGTAGYQEVEVNSRGRVVRT
LKYVPPIPGKDIVLNLDIEIQKFVAKQLDGRRGAAIVLEPNTSAVLAMVSSPSYDPNLFVHGISSKNYNALLNNPDRPLV
NRATLGIYPPASTVKPFIAVSALEEKIVTPLTTRNDPGYWRIPNSKTRPFRDWKRWGHGTVNLKQAIEESVDTFFYQISF
DMGIDRLSAWMMKFGFGDYTGIDIHEESKANMPTRDWKVARFRTPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNRGK
VQAPHLLKTIIDGNVETPFEPEPYPPIEGIRDSYWQLAIDGMILVNNGAKGTARRVFAGTPYVSAGKSGSSQVFGLNGQE
YNADEIAERLRDHALFTGFAPAKDPKVIISLVLENGGGGSSQGGPIARSVFDHVLLPATGEVSNK

Sequences:

>Translated_625_residues
MFKRRRTQIRDYHAESRLFVSRAIVSFVGILVLISLLLTNLYNIQINEFQDYKTRSNDNRIKIVPIAPNRGLIYDRNRMP
LAENRPVFNLEITPDKVKDLSALFTEIQTILPISQDNIDDFTKERRRTRRFKSIPILSQLTQEQVAIFSVNQHKFSGVEI
KAYLKRFYPYGKTFTHVLGYVAKINDRDLARLAREEKDANYQATRDIGKLGIERYYEDTLHGTAGYQEVEVNSRGRVVRT
LKYVPPIPGKDIVLNLDIEIQKFVAKQLDGRRGAAIVLEPNTSAVLAMVSSPSYDPNLFVHGISSKNYNALLNNPDRPLV
NRATLGIYPPASTVKPFIAVSALEEKIVTPLTTRNDPGYWRIPNSKTRPFRDWKRWGHGTVNLKQAIEESVDTFFYQISF
DMGIDRLSAWMMKFGFGDYTGIDIHEESKANMPTRDWKVARFRTPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNRGK
VQAPHLLKTIIDGNVETPFEPEPYPPIEGIRDSYWQLAIDGMILVNNGAKGTARRVFAGTPYVSAGKSGSSQVFGLNGQE
YNADEIAERLRDHALFTGFAPAKDPKVIISLVLENGGGGSSQGGPIARSVFDHVLLPATGEVSNK
>Mature_625_residues
MFKRRRTQIRDYHAESRLFVSRAIVSFVGILVLISLLLTNLYNIQINEFQDYKTRSNDNRIKIVPIAPNRGLIYDRNRMP
LAENRPVFNLEITPDKVKDLSALFTEIQTILPISQDNIDDFTKERRRTRRFKSIPILSQLTQEQVAIFSVNQHKFSGVEI
KAYLKRFYPYGKTFTHVLGYVAKINDRDLARLAREEKDANYQATRDIGKLGIERYYEDTLHGTAGYQEVEVNSRGRVVRT
LKYVPPIPGKDIVLNLDIEIQKFVAKQLDGRRGAAIVLEPNTSAVLAMVSSPSYDPNLFVHGISSKNYNALLNNPDRPLV
NRATLGIYPPASTVKPFIAVSALEEKIVTPLTTRNDPGYWRIPNSKTRPFRDWKRWGHGTVNLKQAIEESVDTFFYQISF
DMGIDRLSAWMMKFGFGDYTGIDIHEESKANMPTRDWKVARFRTPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNRGK
VQAPHLLKTIIDGNVETPFEPEPYPPIEGIRDSYWQLAIDGMILVNNGAKGTARRVFAGTPYVSAGKSGSSQVFGLNGQE
YNADEIAERLRDHALFTGFAPAKDPKVIISLVLENGGGGSSQGGPIARSVFDHVLLPATGEVSNK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=614, Percent_Identity=57.0032573289902, Blast_Score=752, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=627, Percent_Identity=22.0095693779904, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70430; Mature: 70430

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRRRTQIRDYHAESRLFVSRAIVSFVGILVLISLLLTNLYNIQINEFQDYKTRSNDNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHCCCCCCE
IKIVPIAPNRGLIYDRNRMPLAENRPVFNLEITPDKVKDLSALFTEIQTILPISQDNIDD
EEEEEECCCCCEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHH
FTKERRRTRRFKSIPILSQLTQEQVAIFSVNQHKFSGVEIKAYLKRFYPYGKTFTHVLGY
HHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
VAKINDRDLARLAREEKDANYQATRDIGKLGIERYYEDTLHGTAGYQEVEVNSRGRVVRT
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEE
LKYVPPIPGKDIVLNLDIEIQKFVAKQLDGRRGAAIVLEPNTSAVLAMVSSPSYDPNLFV
EECCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCCCEEE
HGISSKNYNALLNNPDRPLVNRATLGIYPPASTVKPFIAVSALEEKIVTPLTTRNDPGYW
EEECCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
RIPNSKTRPFRDWKRWGHGTVNLKQAIEESVDTFFYQISFDMGIDRLSAWMMKFGFGDYT
ECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCC
GIDIHEESKANMPTRDWKVARFRTPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNRGK
CCEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCC
VQAPHLLKTIIDGNVETPFEPEPYPPIEGIRDSYWQLAIDGMILVNNGAKGTARRVFAGT
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCEEEEECCEEEEECCCCCCCCEEEECC
PYVSAGKSGSSQVFGLNGQEYNADEIAERLRDHALFTGFAPAKDPKVIISLVLENGGGGS
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEECCCCCC
SQGGPIARSVFDHVLLPATGEVSNK
CCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFKRRRTQIRDYHAESRLFVSRAIVSFVGILVLISLLLTNLYNIQINEFQDYKTRSNDNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHCCCCCCE
IKIVPIAPNRGLIYDRNRMPLAENRPVFNLEITPDKVKDLSALFTEIQTILPISQDNIDD
EEEEEECCCCCEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHH
FTKERRRTRRFKSIPILSQLTQEQVAIFSVNQHKFSGVEIKAYLKRFYPYGKTFTHVLGY
HHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
VAKINDRDLARLAREEKDANYQATRDIGKLGIERYYEDTLHGTAGYQEVEVNSRGRVVRT
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEE
LKYVPPIPGKDIVLNLDIEIQKFVAKQLDGRRGAAIVLEPNTSAVLAMVSSPSYDPNLFV
EECCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCCCCEEE
HGISSKNYNALLNNPDRPLVNRATLGIYPPASTVKPFIAVSALEEKIVTPLTTRNDPGYW
EEECCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
RIPNSKTRPFRDWKRWGHGTVNLKQAIEESVDTFFYQISFDMGIDRLSAWMMKFGFGDYT
ECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCC
GIDIHEESKANMPTRDWKVARFRTPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNRGK
CCEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCC
VQAPHLLKTIIDGNVETPFEPEPYPPIEGIRDSYWQLAIDGMILVNNGAKGTARRVFAGT
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCEEEEECCEEEEECCCCCCCCEEEECC
PYVSAGKSGSSQVFGLNGQEYNADEIAERLRDHALFTGFAPAKDPKVIISLVLENGGGGS
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEECCCCCC
SQGGPIARSVFDHVLLPATGEVSNK
CCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]