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The map label for this gene is rlmH

Identifier: 209694549

GI number: 209694549

Start: 1089894

End: 1090364

Strand: Reverse

Name: rlmH

Synonym: VSAL_I0986

Alternate gene names: 209694549

Gene position: 1090364-1089894 (Counterclockwise)

Preceding gene: 209694550

Following gene: 209694548

Centisome position: 32.79

GC content: 42.68

Gene sequence:

>471_bases
ATGAAACTTCAATTAATTGCTGTTGGCACTAAAATGCCTAAATGGGTAGAAGAAGGTTATAAAGAATATAGCCGTCGCTT
CCCAAAAGACATGCCTCTTGAACTTATAGAGATCACCGCAGGTAAACGCGGTAAAAATGCAGATATAGCACGAATTCTTC
AAAAAGAAGGAGAAGCAATGCTTGCTGCCGTTCCTAAAGGAAACCGCATTGTCACGTTAGACATCCCAGGTAAACCTTGG
AATACAGAACAACTAGCAGAACAATTAGAAGTCTGGAAATTAGATGCTCGCGATGTATCAATTCTTATTGGTGGTCCTGA
AGGTTTATCTCCCGCTTGTAAAACCGCAGCAGAGCAAAGTTGGTCTTTGTCACCATTAACGCTTCCTCACCCTCTTGTTC
GTGTAGTTATGGCTGAAAGCCTGTACCGAGCATGGAGTATTACGGCCAACCACCCTTACCACAGAGAATAA

Upstream 100 bases:

>100_bases
AGGTGAATGGGTTGTTGTCGATATGGGTGATGTAATGATCCATATTTTACAAGCTGAGCACCGTGAGCTATACCAACTTG
AAAAACTGTGGAGCTAATCC

Downstream 100 bases:

>100_bases
GAGTTGTTTAAGCGCAGACGCACACAGATACGTGACTATCATGCAGAATCGAGATTATTTGTTTCTAGAGCAATAGTCTC
TTTTGTCGGGATATTGGTTC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKLQLIAVGTKMPKWVEEGYKEYSRRFPKDMPLELIEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIVTLDIPGKPW
NTEQLAEQLEVWKLDARDVSILIGGPEGLSPACKTAAEQSWSLSPLTLPHPLVRVVMAESLYRAWSITANHPYHRE

Sequences:

>Translated_156_residues
MKLQLIAVGTKMPKWVEEGYKEYSRRFPKDMPLELIEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIVTLDIPGKPW
NTEQLAEQLEVWKLDARDVSILIGGPEGLSPACKTAAEQSWSLSPLTLPHPLVRVVMAESLYRAWSITANHPYHRE
>Mature_156_residues
MKLQLIAVGTKMPKWVEEGYKEYSRRFPKDMPLELIEITAGKRGKNADIARILQKEGEAMLAAVPKGNRIVTLDIPGKPW
NTEQLAEQLEVWKLDARDVSILIGGPEGLSPACKTAAEQSWSLSPLTLPHPLVRVVMAESLYRAWSITANHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=82.6923076923077, Blast_Score=263, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ALISL (B6EIM8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262477.1
- ProteinModelPortal:   B6EIM8
- GeneID:   6988830
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0986
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17498; Mature: 17498

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQLIAVGTKMPKWVEEGYKEYSRRFPKDMPLELIEITAGKRGKNADIARILQKEGEAM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEE
LAAVPKGNRIVTLDIPGKPWNTEQLAEQLEVWKLDARDVSILIGGPEGLSPACKTAAEQS
EEECCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHCC
WSLSPLTLPHPLVRVVMAESLYRAWSITANHPYHRE
CCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure
MKLQLIAVGTKMPKWVEEGYKEYSRRFPKDMPLELIEITAGKRGKNADIARILQKEGEAM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEE
LAAVPKGNRIVTLDIPGKPWNTEQLAEQLEVWKLDARDVSILIGGPEGLSPACKTAAEQS
EEECCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHCC
WSLSPLTLPHPLVRVVMAESLYRAWSITANHPYHRE
CCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA